Macrophage, human

Monocyte-derived. Macrophages are immune cells found all over the body. They act as scavengers that engulf dead cells, foreign substances, and other debris.

Cell Type:
Macrophage
Tissue Origin:
blood
Species:
human
Research Area:
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P3 96-DP-148 1e5 40-44% 52-68% Plasmid (general) 0.4 µg 20 µl Shuttle
P3

DP-148

1e5 40-44% 52-68% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
Macrophage, human Y-010 5e5 44-74% 79-97% Plasmid (general) 2 µg 100 µl I/II/2b
Macrophage, human Y-010 5e5 44-66% 81-93% Plasmid (general) 2 µg 100 µl I/II/2b

Citations (38)

Categories:
Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Freund EC, Lock JY, Oh J, Maculins T, Delamarre L, Bohlen CJ, Haley B, Murthy A. 
In:
J Exp Med (2020) 217(7): e20191692 
Categories:
Primary Cells and Media, 3D Cell Culture , Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chandorkar P, Posch W, Zaderer V, Blatzer M, Steger M, Ammann CG, Binder U, Hermann M, Hörtnagl P, Lass-Flörl C, Wilflingseder D 
In:
Scientific Reports (2017) 7(1): 10.1038/s41598-017-11271-4 
Categories:
Serum-free and Speciality Media 
Authors:
Ellen J. Lee, Brieanna R. Brown, Emily E. Vance, Paige E. Snow, Phyllis B. Silver, David Heinrichs, Xin Lin, Yoichiro Iwakura, Christine A. Wells, Rachel R. Caspi and Holly L. Rosenzweig 
In:
J Immunol (2016) 196: 7 
Categories:
Transfection 
Authors:
Tallam A, Perumal TM, Antony PM, Jäger C, Fritz JV, Vallar L, Balling R, Del Sol A, Michelucci A. 
In:
PLoS ONE (2016) 11(2): 1-28 
Categories:
Primary Cells and Media 
Authors:
Jones A, Kainz D, Khan F, Lee C, Carrithers MD 
In:
J Biol Chem (2014) 289(51): 35326-40 
Categories:
Primary Cells and Media 
Authors:
Gamez, JD, Voloshyna I, Littlefield MJ, Castro-Magana M, Reiss AB 
In:
Other (2014) S11:005: ePub 
Categories:
 
Authors:
Valentine CD, Zhang H, Phuan PW, Nguyen J, Verkman AS, Haggie PM. 
In:
Cell Microbiol (2014) 16(1): 1-14 
Categories:
Primary Cells and Media 
Authors:
Dashty M, Akbarkhanzadeh V, Zeebregts CJ, Spek CA, Sijbrands EJ, Peppelenbosch MP, Rezaee F. 
In:
Scientific Reports (2012) 2(787): ePub 
Categories:
Primary Cells and Media 
Authors:
Wahl-Jensen V, Kurz S, Feldmann F, Buehler LK, Kindrachuk J, DeFilippis V, da Silva Correia J, Früh K, Kuhn JH, Burton DR, Feldmann H. 
In:
Other (2011) 5(10): ePub 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Bioassay  
Authors:
Ueland T, Lekva T, Otterdal K, Dahl TB, Olarescu NC, Jørgensen AP, Fougner KJ, Brixen K, Aukrust P, Bollerslev J 
In:
Eur J Endocrinol (2011) 165(3): 393-400 
Categories:
Primary Cells and Media, Transfection 
Authors:
Borthwick F, Allen AM, Taylor JM, Graham A 
In:
Other (2010) 119(7): 265-72 
Categories:
Transfection 
Authors:
Schnoor M, Buers I, Sietmann A, Brodde MF, Hofnagel O, Robenek H, Lorkowski S. 
In:
J Immunol Methods (2009) 344(2): 109-15 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Knapek KJ 
In:
Other (2008) 0:0: Thesis 
Categories:
Transfection 
Authors:
Tachado SD, Li X, Swan K, Patel N, Koziel H 
In:
J Biol Chem (2008) 283(48): 33191-8 
Categories:
Transfection 
Authors:
Poole E, Atkins E, Nakayama T, Yoshie O, Groves I, Alcami A, Sinclair J 
In:
J Virol (2008) 82(9): 4250-6 
Categories:
Transfection 
Authors:
Ruiz-Mateos E, Pelchen-Matthews A, Deneka M, Marsh M 
In:
J Virol (2008) 82(10): 4751-61 
Categories:
Transfection 
Authors:
Vinzing M, Eitel J, Lippmann J, Hocke AC, Zahlten J, Slevogt H, N'guessan PD, Günther S, Schmeck B, Hippenstiel S, Flieger A, Suttorp N, Opitz B 
In:
J Immunol (2008) 180(10): 6808-15 
Categories:
Transfection 
Authors:
Cheung R, Ravyn V, Wang L, Ptasznik A, Collman RG 
In:
J Immunol (2008) 180(10): 6675-84 
Categories:
Transfection 
Authors:
Chen ST, Lin YL, Huang MT, Wu MF, Cheng SC, Lei HY, Lee CK, Chiou TW, Wong CH, Hsieh SL 
In:
Nature (2008) 453(7195): 672-6 
Categories:
Transfection 
Authors:
El Kasmi KC, Smith AM, Williams L, Neale G, Panopolous A, Watowich SS, Häcker H, Foxwell BM, Murray PJ 
In:
J Immunol (2007) 179(11): 7215-7219 
Categories:
Transfection 
Authors:
Hedl M, Li J, Cho JH, Abraham C 
In:
Proc Natl Acad Sci USA (2007) 104(49): 19440-5 
Categories:
Transfection 
Authors:
Tsuboi S, Meerloo J 
In:
J Biol Chem (2007) 282(47): 34194-203 
Categories:
Transfection 
Authors:
Jacquot G, le Rouzic E, David A, Mazzolini J, Bouchet J, Bouaziz S, Niedergang F, Pancino G, Benichou S 
In:
Retrovirology (2007) 4(1): 84 
Categories:
Transfection 
Authors:
Dhillon NK, Peng F, Ransohoff RM, Buch S 
In:
J Immunol (2007) 179(5): 2722-30 
Categories:
Transfection 
Authors:
Reimer T, Schweizer M, Jungi TW 
In:
J Immunol (2007) 179(2): 1166-77 
Categories:
Transfection 
Authors:
Pompei L, Jang S, Zamlynny B, Ravikumar S, McBride A, Hickman SP, Salgame P 
In:
J Immunol (2007) 178(8): 5192-9 
Categories:
Transfection 
Authors:
Deneka M, Pelchen-Matthews A, Byland R, Ruiz-Mateos E, Marsh M 
In:
J Cell Biol (2007) 177(2): 329-41 
Categories:
Transfection 
Authors:
Park-Min KH, Serbina NV, Yang W, Ma X, Krystal G, Neel BG, Nutt SL, Hu X, Ivashkiv LB 
In:
Immunity (2007) 26(1): 67-78 
Categories:
Transfection 
Authors:
Onfelt B, Nedvetzki S, Benninger RK, Purbhoo MA, Sowinski S, Hume AN, Seabra MC, Neil MA, French PM, Davis DM 
In:
J Immunol (2006) 177(12): 8476-83 
Categories:
Transfection 
Authors:
Jouvenet N, Neil SJ, Bess C, Johnson MC, Virgen CA, Simon SM, Bieniasz PD 
In:
PLoS Biol (2006) 4(12): e435 
Categories:
Transfection 
Authors:
Kopp P, Lammers R, Aepfelbacher M, Woehlke G, Rudel T, Machuy N, Steffen W, Linder S 
In:
Mol Biol Cell (2006) 17(6): 2811-2823 
Categories:
Transfection 
Authors:
Hu X, Paik PK, Chen J, Yarilina A, Kockeritz L, Lu TT, Woodgett JR, Ivashkiv LB 
In:
Immunity (2006) 24(5): 563-74 
Categories:
Transfection 
Authors:
Kzhyshkowska J, Mamidi S, Gratchev A, Kremmer E, Schmuttermaier C, Krusell L, Haus G, Utikal J, Schledzewski K, Scholtze J and Goerdt S 
In:
Blood (2006) 107(8): 3221-8 
Categories:
Transfection 
Authors:
Du Z, Kelly E, Mecklenbrauker I, Agle L, Herrero C, Paik P, Ivashkiv LB 
In:
J Immunol (2006) 176(8): 4785-92 
Categories:
Transfection 
Authors:
Muthumani K, Choo AY, Zong WX, Madesh M, Hwang DS, Premkumar A, Thieu KP, Emmanuel J, Kumar S, Thompson CB, Weiner DB 
In:
Nat Cell Biol (2006) 8(2): 170-9 
Categories:
Transfection 
Authors:
Hernandez LD, Pypaert M, Flavell RA and Galan JE 
In:
J Cell Biol (2003) 163(5): 1123-1131 
Categories:
Transfection 
Authors:
Kondo K, Sashhara J, Shimada K, Takemoto M, Amo K, Miyagawa H and Yamanishi K 
In:
J Virol (2003) 77(3): 2258-2264 
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