cells

chronic lymphatic leukemia

Cell Type:

B cell

Tissue Origin:

blood

Species:

human

Research Area:

Cancer Research/Cell Biology

Immunotherapy / Hematology

Cell Characteristics:

Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Protocol	Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
	R	U-016	5e6	20-60%	90%	Plasmid (general)	5 µg	100 µl	I/II/2b
	V	U-007	3e6	30%	75%	Plasmid (general)	2.5 µg	100 µl	I/II/2b

Citations (11)

Gene knockdown studies revealed CCDC50 as a candidate gene in mantle cell lymphoma and chronic lymphocytic leukemia

Categories:

Transfection

Authors:

Farfsing A, Engel F, Seiffert M, Hartmann E, Ott G, Rosenwald A, Stilgenbauer S, Döhner H, Boutros M, Lichter P, Pscherer A.

In:

Leukemia (2009) 23(11): 2018-26

Interactions between Bortezomib and Romidepsin and Belinostat in Chronic Lymphocytic Leukemia Cells

Categories:

Transfection

Authors:

Dai Y, Chen S, Kramer LB, Funk VL, Dent P, Grant S

In:

Clin Cancer Res (2008) 14(2): 549-58

Homeostatic chemokines increase survival of B-chronic lymphocytic leukemia cells through inactivation of transcription factor FOXO3a

Categories:

Transfection

Authors:

Ticchioni M, Essafi M, Jeandel PY, Davi F, Cassuto JP, Deckert M, Bernard A

In:

Oncogene (2007) 26(50): 7081-91

Efficient nucleofection of primary human B cells and B-CLL cells induces apoptosis, which depends on the microenvironment and on the structure of transfected nucleic acids

Categories:

Transfection

Authors:

Seiffert M, Stilgenbauer S, Döhner H, Lichter P

In:

Leukemia (2007) 21(9): 1977-83

ZAP-70 enhances B-cell receptor signaling in spite of absent or inefficient tyrosine kinase activation in chronic lymphocytic leukemia and lymphoma B-cells

Categories:

Transfection

Authors:

Gobessi S, Laurenti L, Longo PG, Sica S, Leone G, Efremov DG

In:

Blood (2007) 109(5): 2032-9

BAFF And APRIL Support Chronic Lymphocytic Leukemia B Cell Survival Through Activation Of The Canonical NF-B Pathway

Categories:

Transfection

Authors:

Endo T, Nishio M, Enzler T, Cottam HB, Fukuda T, James DF, Karin M, Kipps TJ

In:

Blood (2007) 109(2): 703-10

The Akt signaling pathway determines the different proliferative capacity of chronic lymphocytic leukemia B-cells from patients with progressive and stable disease

Categories:

Transfection

Authors:

Longo PG, Laurenti L, Gobessi S, Petlickovski A, Pelosi M, Chiusolo P, Sica S, Leone G, Efremov DG

In:

Leukemia (2007) 21(1): 110-120

Role of BAFF and APRIL in human B-cell chronic lymphocytic leukaemia

Categories:

Transfection

Authors:

Haiat S, Billard C, Quiney C, Ajchenbaum-Cymbalista F, Kolb JP

In:

Immunology (2006) 118(3): 281-92

Chronic lymphocytic leukemia cells induce changes in gene expression of CD4 and CD8 T cells

Categories:

Transfection

Authors:

Gorgun G, Holderried TA, Zahrieh D, Neuberg D and Gribben JG

In:

J Clin Invest (2005) 115(7): 1797-1805

Type 4 cAMP phosphodiesterase (PDE4) inhibitors augment glucocorticoid-mediated apoptosis in B cell chronic lymphocytic leukemia (B-CLL) in the absence of exogenous adenylyl cyclase stimulation

Categories:

Transfection

Authors:

Tiwari S, Dong H, Kim EJ, Weintraub L, Epstein PM and Lerner A

In:

Biochem Pharmacol (2005) 69(3): 473-483

New non-viral method for gene transfer into primary cells

Categories:

Transfection

Authors:

Gresch O, Engel FB, Nesic D, Tran TT, England HM, Hickman ES, Korner I, Gan L, Chen S, Castro-Obregon S, Hammermann R, Wolf J, Muller-Hartmann H, Nix M, Siebenkotten G, Kraus G and Lun K

In:

Methods (2004) 33(2): 151-163

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