Embryonic fibroblast, mouse (MEF) immort

Primary fibroblasts immortalized by frequent passaging or SV40 transformation.

Cell Type:
Fibroblast
Tissue Origin:
embryo
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Recommended Media

Dulbecco's Modified Eagle Medium (DMEM) was developed in 1969 and is a modification of Basal Medium Eagle (BME) that differs from BME and MEM by the following characteristics:

  • Vitamins 4X greater than MEM. Vitamins and amino acids greater than BME
  • Types and quantities of amino acids greater than MEM and BME
  • Iron (ferric nitrate)

It is used in a wide range of mammalian cell culture applications.  The high glucose version is well suited to high density suspension culture. The low glucose formula is used for adherent dependent cells.

Storage = 2ºC to 8ºC

Liquid:
12-614 = 4.5 g/L glucose, without L-glutamine
12-604 = 4.5 g/L glucose, with L-glutamine
BE12-604/U1 = 4.5 g/L glucose, with Ultraglutamine I
12-914 = 4.5 g/L glucose, without L-glutamine (hybridoma screened)
12-917 = 4.5 g/L glucose, without L-glutamine or phenol red
12-709 = 4.5 g/L glucose and 25 mM HEPES, without L-glutamine
12-733= 4.5 g/L glucose, without L-glutamine or sodium pyruvate
12-741 = 4.5 g/L glucose, with L-glutamine, without sodium pyruvate

12-707 = 1.0 g/L glucose, without L-glutamine
12-708 = 1.0 g/L glucose and 25 mM HEPES, without L-glutamine

Powder:
15-614 = 4.5 g/L glucose, without L-glutamine or sodium pyruvate
15-604 = 4.5 g/L glucose, with L-glutamine or sodium pyruvate

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
MEF 1 A-023 2e6 19-29% 60-80% Plasmid (general) 1.5 µg 100 µl I/II/2b
MEF 2 A-023 2e6 35-51% 60-80% Plasmid (general) 7 µg 100 µl I/II/2b
P2 DS-137 2e5 40-60% 60-80% Plasmid (general) 0.4 µg 20 µl Shuttle
P2 EN-150 1e5 60-64% 58-80% Plasmid (general) 0.4 µg 20 µl Shuttle
P3 EH-100 1e5 44-50% 72-104% Plasmid (general) 0.4 µg 20 µl Shuttle

Citations (72)

Categories:
Transfection 
Authors:
Holmes O1, Paturi S, Wolfe MS, Selkoe DJ. 
In:
J Neurochem (2014) 131 (1): 94-100 
Categories:
 
Authors:
Valentine CD, Zhang H, Phuan PW, Nguyen J, Verkman AS, Haggie PM. 
In:
Cell Microbiol (2014) 16(1): 1-14 
Categories:
Transfection 
Authors:
Kaji K, Norrby K, Paca A, Mileikovsky M, Mohseni P, Woltjen K 
In:
Nature (2009) epub: online 
Categories:
Transfection 
Authors:
Vincenz C, Kerppola TK 
In:
Proc Natl Acad Sci USA (2008) 105(43): 16572-7 
Categories:
Transfection 
Authors:
Hayakawa F, Abe A, Kitabayashi I, Pandolfi PP, Naoe T 
In:
J Biol Chem (2008) epub: online 
Categories:
Transfection 
Authors:
Lange SS, Mitchell DL, Vasquez KM 
In:
Proc Natl Acad Sci USA (2008) 105(30): 10320-5 
Categories:
Transfection 
Authors:
Dong M, Bridges JP, Apsley K, Xu Y, Weaver TE 
In:
Mol Biol Cell (2008) 19(6): 2620-30 
Categories:
Transfection 
Authors:
Liu P, Li K, Garofalo RP, Brasier AR 
In:
J Biol Chem (2008) epub: online 
Categories:
Transfection 
Authors:
Conze DB, Wu CJ, Thomas JA, Landstrom A, Ashwell JD 
In:
Mol Cell Biol (2008) 28(10): 3538-47 
Categories:
Transfection 
Authors:
Nakamura T, Hayashi T, Nasu-Nishimura Y, Sakaue F, Morishita Y, Okabe T, Ohwada S, Matsuura K, Akiyama T 
In:
Genes Dev (2008) 22(9): 1244-56 
Categories:
Transfection 
Authors:
Windheim M, Stafford M, Peggie M, Cohen P 
In:
Mol Cell Biol (2008) 28(5): 1783-91 
Categories:
Transfection 
Authors:
Toyo-Oka K, Mori D, Yano Y, Shiota M, Iwao H, Goto H, Inagaki M, Hiraiwa N, Muramatsu M, Wynshaw-Boris A, Yoshiki A, Hirotsune S 
In:
J Cell Biol (2008) 180(6): 1133-47 
Categories:
Transfection 
Authors:
Pfeifer I, Elsby R, Fernandez M, Faria PA, Nussenzveig DR, Lossos IS, Fontoura BM, Martin WD, Barber GN 
In:
Proc Natl Acad Sci USA (2008) 105(11): 4173-8 
Categories:
Transfection 
Authors:
Wu YT, Zhang S, Kim YS, Tan HL, Whiteman M, Ong CN, Liu ZG, Ichijo H, Shen HM 
In:
Cell Death Differ (2008) 15(2): 386-97 
Categories:
Transfection 
Authors:
Nyfeler B, Reiterer V, Wendeler MW, Stefan E, Zhang B, Michnick SW, Hauri HP 
In:
J Cell Biol (2008) 180(4): 705-12 
Categories:
Transfection 
Authors:
Reef S, Shifman O, Oren M, Kimchi A 
In:
Oncogene (2007) 26(46): 6677-83 
Categories:
Transfection 
Authors:
Vitte J, Fassier C, Tiziano FD, Dalard C, Soave S, Roblot N, Brahe C, Saugier-Veber P, Bonnefont JP, Melki J 
In:
Am J Pathol (2007) 171(4): 1269-80 
Categories:
Transfection 
Authors:
von Holzen U, Pataer A, Raju U, Bocangel D, Vorburger SA, Liu Y, Lu X, Roth JA, Aggarwal BB, Barber GN, Keyomarsi K, Hunt KK, Swisher SG 
In:
Clin Cancer Res (2007) 13(20): 6032-9 
Categories:
Transfection 
Authors:
Gills JJ, Castillo SS, Zhang C, Petukhov PA, Memmott RM, Hollingshead M, Warfel N, Han J, Kozikowski AP, Dennis PA 
In:
J Biol Chem (2007) 282(37): 27020-9 
Categories:
Transfection 
Authors:
Huttunen HJ, Guénette SY, Peach C, Greco C, Xia W, Kim DY, Barren C, Tanzi RE, Kovacs DM 
In:
J Biol Chem (2007) 282(38): 28285-95 
Categories:
Transfection 
Authors:
Xiong L, Kou F, Yang Y, Wu J 
In:
J Cell Biol (2007) 178(6): 995-1007 
Categories:
Transfection 
Authors:
Lue H, Thiele M, Franz J, Dahl E, Speckgens S, Leng L, Fingerle-Rowson G, Bucala R, Luscher B, Bernhagen J 
In:
Oncogene (2007) 26(35): 5046-59 
Categories:
Transfection 
Authors:
Donigian JR, de Lange T 
In:
J Biol Chem (2007) 282(31): 22662-7 
Categories:
Transfection 
Authors:
Jounai N, Takeshita F, Kobiyama K, Sawano A, Miyawaki A, Xin KQ, Ishii KJ, Kawai T, Akira S, Suzuki K, Okuda K 
In:
Proc Natl Acad Sci USA (2007) 104(35): 14050-5 
Categories:
Transfection 
Authors:
Moubarak RS, Yuste VJ, Artus C, Bouharrour A, Greer PA, Menissier-de Murcia J, Susin SA 
In:
Mol Cell Biol (2007) 27(13): 4844-62 
Categories:
Transfection 
Authors:
Windheim M, Lang C, Peggie M, Cummings L, Cohen P 
In:
Biochem J (2007) 404(2): 179-90 
Categories:
Transfection 
Authors:
Mizuta T, Shimizu S, Matsuoka Y, Nakagawa T, Tsujimoto Y 
In:
J Biol Chem (2007) 282(22): 16623-16630 
Categories:
Transfection 
Authors:
Zhang S, Lin Y, Kim YS, Hande MP, Liu ZG, Shen HM 
In:
Cell Death Differ (2007) 14(5): 1001-10 
Categories:
Transfection 
Authors:
Noels H, van Loo G, Hagens S, Broeckx V, Beyaert R, Marynen P, Baens M 
In:
J Biol Chem (2007) 282(14): 10180-9 
Categories:
Transfection 
Authors:
Wixler V, Hirner S, Muller JM, Gullotti L, Will C, Kirfel J, Gunther T, Schneider H, Bosserhoff A, Schorle H, Park J, Schule R, Buettner R 
In:
J Cell Biol (2007) 177(1): 163-72 
Categories:
Transfection 
Authors:
Orth JH, Aktories K, Kubatzky KF 
In:
J Biol Chem (2007) 282(5): 3050-7 
Categories:
Transfection 
Authors:
Sunyach C, Alfa Cisse M, Alves da Costa C, Vincent B, Checler F 
In:
J Biol Chem (2007) 282(3): 1956-1963 
Categories:
Transfection 
Authors:
Wang X, Trotman LC, Koppie T, Alimonti A, Chen Z, Gao Z, Wang J, Erdjument-Bromage H, Tempst P, Cordon-Cardo C, Pandolfi PP, Jiang X 
In:
Cell (2007) 128(1): 129-39 
Categories:
Transfection 
Authors:
Wang M, Wu W, Wu W, Rosidi B, Zhang L, Wang H, Iliakis G 
In:
Nucleic Acids Res (2006) 34(21): 6170-82 
Categories:
Transfection 
Authors:
Stumpf M, Waskow C, Krotschel M, van Essen D, Rodriguez P, Zhang X, Guyot B, Roeder RG, Borggrefe T 
In:
Proc Natl Acad Sci USA (2006) 103(49): 18504-9 
Categories:
Transfection 
Authors:
Leitner NR, Strobl B, Bokor M, Painz R, Kolbe T, Rulicke T, Muller M, Karaghiosoff M 
In:
BMC Biotechnol (2006) 6: 48 
Categories:
Transfection 
Authors:
Poulikakos PI, Xiao GH, Gallagher R, Jablonski S, Jhanwar SC, Testa JR 
In:
Oncogene (2006) 25(44): 5960-8 
Categories:
Transfection 
Authors:
Zhou J, Liu CY, Back SH, Clark RL, Peisach D, Xu Z, Kaufman RJ 
In:
Proc Natl Acad Sci USA (2006) 103(39): 14343-8 
Categories:
Transfection 
Authors:
Zaidi NF, Kuplast KG, Washicosky KJ, Kajiwara Y, Buxbaum JD, Wasco W 
In:
J Neurochem (2006) 98(4): 1290-301 
Categories:
Transfection 
Authors:
Gaus K, Le Lay S, Balasubramanian N, Schwartz MA 
In:
J Cell Biol (2006) 174(5): 725-34 
Categories:
Transfection 
Authors:
Back SH, Lee K, Vink E, Kaufman RJ 
In:
J Biol Chem (2006) 281(27): 18691-706 
Categories:
Transfection 
Authors:
Saha SK, Pietras EM, He JQ, Kang JR, Liu SY, Oganesyan G, Shahangian A, Zarnegar B, Shiba TL, Wang Y, Cheng G 
In:
EMBO J (2006) 25(14): 3257-63 
Categories:
Transfection 
Authors:
Phillips SE, Ile KE, Boukhelifa M, Huijbregts RP, Bankaitis VA 
In:
Mol Biol Cell (2006) 17(6): 2498-2512 
Categories:
Transfection 
Authors:
Boyer L, Turchi L, Desnues B, Doye A, Ponzio G, Mege JL, Yamashita M, Zhang YE, Bertoglio J, Flatau G, Boquet P, Lemichez E 
In:
Mol Biol Cell (2006) 17(6): 2489-2497 
Categories:
Transfection 
Authors:
Nyborg AC, Ladd TB, Zwizinski CW, Lah JJ, Golde TE 
In:
Mol Neurodegener (2006) 1: 3 
Categories:
Transfection 
Authors:
Duensing A, Liu Y, Tseng M, Malumbres M, Barbacid M, Duensing S 
In:
Oncogene (2006) 25(20): 2943-9 
Categories:
Transfection 
Authors:
Greenberg RS, Bernstein AM, Benezra M, Gelman IH, Taliana L, Masur SK 
In:
FASEB J (2006) 20(7): 1006-8 
Categories:
Transfection 
Authors:
Chen X, Zhang J, Lee J, Lin PS, Ford JM, Zheng N, Zhou P 
In:
Mol Cell (2006) 22(4): 489-99 
Categories:
Transfection 
Authors:
Janiak A, Zemskov EA and Belkin AM 
In:
Mol Biol Cell (2006) 17(4): 1606-19 
Categories:
Transfection 
Authors:
Svard J, Heby-Henricson K, Persson-Lek M, Rozell B, Lauth M, Bergstrom A, Ericson J, Toftgard R, Teglund S 
In:
Dev Cell (2006) 10(2): 187-97 
Categories:
Transfection 
Authors:
Khanday FA, Yamamori T, Mattagajasingh I, Zhang Z, Bugayenko A, Naqvi A, Santhanam L, Nabi N, Kasuno K, Day BW and Irani K 
In:
Mol Biol Cell (2006) 17(1): 122-129 
Categories:
Transfection 
Authors:
Mercer K, Giblett S, Green S, Lloyd D, Darocha Dias S, Plumb M, Marais R and Pritchard C 
In:
Cancer Res (2005) 65(24): 11493-11500 
Categories:
Transfection 
Authors:
Orth JH, Lang S, Taniguchi M and Aktories K 
In:
J Biol Chem (2005) 280(44): 36701-36707 
Categories:
Transfection 
Authors:
Takahashi Y, Karbowski M, Yamaguchi H, Kazi A, Wu J, Sebti SM, Youle RJ and Wang HG 
In:
Mol Cell Biol (2005) 25(21): 9369-9382 
Categories:
Transfection 
Authors:
Zawistowski JS, Stalheim L, Uhlik MT, Abell AN, Ancrile BB, Johnson GL and Marchuk DA 
In:
Hum Mol Genet (2005) 14(17): 2521-2531 
Categories:
Transfection 
Authors:
Mercer K, Giblett S, Oakden A, Brown J, Marais R and Pritchard C 
In:
Oncogene (2005) 24(33): 5207-5217 
Categories:
Transfection 
Authors:
Wang H, Rosidi B, Perrault R, Wang M, Zhang L, Windhofer F and Iliakis G 
In:
Cancer Res (2005) 65(10): 4020-4030 
Categories:
Transfection 
Authors:
Kisseleva M, Feng Y, Ward M, Song C, Anderson RA and Longmore GD 
In:
Mol Cell Biol (2005) 25(10): 3956-3966 
Categories:
Transfection 
Authors:
Milasta S, Evans NA, Ormiston L, Wilson S, Lefkowitz RJ, Milligan G 
In:
Biochem J (2005) 387(Pt 3): 573-84 
Categories:
Transfection 
Authors:
Pediani JD, Colston JF, Caldwell D, Milligan G, Daly CJ, McGrath JC 
In:
Mol Pharmacol (2005) 67(4): 992-1004 
Categories:
Transfection 
Authors:
Yang W, Wang Q, Howell KL, Lee JT, Cho DS, Murray JM and Nishikura K 
In:
J Biol Chem (2005) 280(5): 3946-3953 
Categories:
Transfection 
Authors:
Pratt SJ, Epple H, Ward M, Feng Y, Braga VM and Longmore GD 
In:
J Cell Biol (2005) 168(5): 813-824 
Categories:
Transfection 
Authors:
Avkin S, Goldsmith M, Velasco-Miguel S, Geacintov N, Friedberg EC and Livneh Z 
In:
J Biol Chem (2004) 279(51): 53298-53305 
Categories:
Transfection 
Authors:
Sugioka R, Shimizu S and Tsujimoto Y 
In:
J Biol Chem (2004) 279(50): 52726-52734 
Categories:
Transfection 
Authors:
Shimizu S, Kanaseki T, Mizushima N, Mizuta T, Arakawa-Kobayashi S, Thompson CB, Tsujimoto Y 
In:
Nat Cell Biol (2004) 6(12): 1221-1228 
Categories:
Transfection 
Authors:
Roth W, Sustmann C, Kieslinger M, Gilmozzi A, Irmer D, Kremmer E, Turck C and Grosschedl R 
In:
J Immunol (2004) 173(10): 6189-6199 
Categories:
Transfection 
Authors:
Zhang L, Shimizu S, Sakamaki K, Yonehara S and Tsujimoto Y 
In:
J Biol Chem (2004) 279(32): 33865-33874 
Categories:
Transfection 
Authors:
Woods AJ, White DP, Caswell PT and Norman JC 
In:
EMBO J (2004) 23(13): 2531-2543 
Categories:
Transfection 
Authors:
Pritchard CA, Hayes L, Wojnowski L, Zimmer A, Marais RM and Norman JC 
In:
Mol Cell Biol (2004) 24(13): 5937-5952 
Categories:
Transfection 
Authors:
Lo CM, Buxton DB, Chua GCH, Dembo M, Adelstein RS and Wang YL 
In:
Mol Biol Cell (2004) 15(3): 982-989 
Categories:
Transfection 
Authors:
Verrecchia F, Wagner EF and Mauviel A 
In:
EMBO Rep (2002) 3(11): 1069-1074 
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