cells

Primary fibroblasts immortalized by frequent passaging or SV40 transformation.

Cell Type:

Fibroblast

Tissue Origin:

embryo

Species:

mouse

Research Area:

Cancer Research/Cell Biology

Cell Characteristics:

Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
MEF 1	A-023	2e6	19-29%	60-80%	Plasmid (general)	1.5 µg	100 µl	I/II/2b
MEF 2	A-023	2e6	35-51%	60-80%	Plasmid (general)	7 µg	100 µl	I/II/2b
P2	DS-137	2e5	40-60%	60-80%	Plasmid (general)	0.4 µg	20 µl	96-well
P2	EN-150	1e5	60-64%	58-80%	Plasmid (general)	0.4 µg	20 µl	96-well
P3	EH-100	1e5	44-50%	72-104%	Plasmid (general)	0.4 µg	20 µl	96-well

Citations (72)

Functional analysis and purification of a Pen-2 fusion protein for ?-secretase structural studies.

Categories:

Transfection

Authors:

Holmes O1, Paturi S, Wolfe MS, Selkoe DJ.

In:

J Neurochem (2014) 131 (1): 94-100

Small molecule screen yields inhibitors of pseudomonas homoserine lactone-induced host responses.

Categories:

Authors:

Valentine CD, Zhang H, Phuan PW, Nguyen J, Verkman AS, Haggie PM.

In:

Cell Microbiol (2014) 16(1): 1-14

Virus-free induction of pluripotency and subsequent excision of reprogramming factors

Categories:

Transfection

Authors:

Kaji K, Norrby K, Paca A, Mileikovsky M, Mohseni P, Woltjen K

In:

Nature (2009) epub: online

Different polycomb group CBX family proteins associate with distinct regions of chromatin using nonhomologous protein sequences

Categories:

Transfection

Authors:

Vincenz C, Kerppola TK

In:

Proc Natl Acad Sci USA (2008) 105(43): 16572-7

Acetylation of PML is involved in histone deacetylase Inhibitor-mediated apoptosis

Categories:

Transfection

Authors:

Hayakawa F, Abe A, Kitabayashi I, Pandolfi PP, Naoe T

In:

J Biol Chem (2008) epub: online

High mobility group protein B1 enhances DNA repair and chromatin modification after DNA damage

Categories:

Transfection

Authors:

Lange SS, Mitchell DL, Vasquez KM

In:

Proc Natl Acad Sci USA (2008) 105(30): 10320-5

ERdj4 and ERdj5 are Required for ERAD of Misfolded Surfactant Protein C

Categories:

Transfection

Authors:

Dong M, Bridges JP, Apsley K, Xu Y, Weaver TE

In:

Mol Biol Cell (2008) 19(6): 2620-30

RSV-induces RelA release from cytoplasmic 100 kDa NF-kappa B2 complexes via a novel retinoic acid inducible gene-I-NF-kappa B inducing kinase (NIK) signaling pathway

Categories:

Transfection

Authors:

Liu P, Li K, Garofalo RP, Brasier AR

In:

J Biol Chem (2008) epub: online

Lys (K)63-linked polyubiquitination of IRAK-1 is required for IL-1 receptor- and Toll-like receptor-mediated NF-kappaB activation

Categories:

Transfection

Authors:

Conze DB, Wu CJ, Thomas JA, Landstrom A, Ashwell JD

In:

Mol Cell Biol (2008) 28(10): 3538-47

PX-RICS mediates ER-to-Golgi transport of the N-cadherin/beta-catenin complex

Categories:

Transfection

Authors:

Nakamura T, Hayashi T, Nasu-Nishimura Y, Sakaue F, Morishita Y, Okabe T, Ohwada S, Matsuura K, Akiyama T

In:

Genes Dev (2008) 22(9): 1244-56

Interleukin-1 (IL-1) induces the Lys63-linked polyubiquitination of IL-1 receptor-associated kinase 1 to facilitate NEMO binding and the activation of IkappaBalpha kinase

Categories:

Transfection

Authors:

Windheim M, Stafford M, Peggie M, Cohen P

In:

Mol Cell Biol (2008) 28(5): 1783-91

Protein phosphatase 4 catalytic subunit regulates Cdk1 activity and microtubule organization via NDEL1 dephosphorylation

Categories:

Transfection

Authors:

Toyo-Oka K, Mori D, Yano Y, Shiota M, Iwao H, Goto H, Inagaki M, Hiraiwa N, Muramatsu M, Wynshaw-Boris A, Yoshiki A, Hirotsune S

In:

J Cell Biol (2008) 180(6): 1133-47

NFAR-1 and -2 modulate translation and are required for efficient host defense

Categories:

Transfection

Authors:

Pfeifer I, Elsby R, Fernandez M, Faria PA, Nussenzveig DR, Lossos IS, Fontoura BM, Martin WD, Barber GN

In:

Proc Natl Acad Sci USA (2008) 105(11): 4173-8

Signaling pathways from membrane lipid rafts to JNK1 activation in reactive nitrogen species-induced non-apoptotic cell death

Categories:

Transfection

Authors:

Wu YT, Zhang S, Kim YS, Tan HL, Whiteman M, Ong CN, Liu ZG, Ichijo H, Shen HM

In:

Cell Death Differ (2008) 15(2): 386-97

Identification of ERGIC-53 as an intracellular transport receptor of alpha1-antitrypsin

Categories:

Transfection

Authors:

Nyfeler B, Reiterer V, Wendeler MW, Stefan E, Zhang B, Michnick SW, Hauri HP

In:

J Cell Biol (2008) 180(4): 705-12

The autophagic inducer smARF interacts with and is stabilized by the mitochondrial p32 protein

Categories:

Transfection

Authors:

Reef S, Shifman O, Oren M, Kimchi A

In:

Oncogene (2007) 26(46): 6677-83

Refined Characterization of the Expression and Stability of the SMN Gene Products

Categories:

Transfection

Authors:

Vitte J, Fassier C, Tiziano FD, Dalard C, Soave S, Roblot N, Brahe C, Saugier-Veber P, Bonnefont JP, Melki J

In:

Am J Pathol (2007) 171(4): 1269-80

The Double-Stranded RNA-Activated Protein Kinase Mediates Radiation Resistance in Mouse Embryo Fibroblasts through Nuclear Factor B and Akt Activation

Categories:

Transfection

Authors:

von Holzen U, Pataer A, Raju U, Bocangel D, Vorburger SA, Liu Y, Lu X, Roth JA, Aggarwal BB, Barber GN, Keyomarsi K, Hunt KK, Swisher SG

In:

Clin Cancer Res (2007) 13(20): 6032-9

Phosphatidylinositol ether lipid analogues that inhibit AKT also independently activate the stress kinase, P38alpha , through MKK3/6-independent and -dependent mechanisms

Categories:

Transfection

Authors:

Gills JJ, Castillo SS, Zhang C, Petukhov PA, Memmott RM, Hollingshead M, Warfel N, Han J, Kozikowski AP, Dennis PA

In:

J Biol Chem (2007) 282(37): 27020-9

Htra2 regulates APP metabolism through endoplasmic reticulum-associated degradation

Categories:

Transfection

Authors:

Huttunen HJ, Guénette SY, Peach C, Greco C, Xia W, Kim DY, Barren C, Tanzi RE, Kovacs DM

In:

J Biol Chem (2007) 282(38): 28285-95

A novel role for IGF-1R in p53-mediated apoptosis through translational modulation of the p53-Mdm2 feedback loop

Categories:

Transfection

Authors:

Xiong L, Kou F, Yang Y, Wu J

In:

J Cell Biol (2007) 178(6): 995-1007

Macrophage migration inhibitory factor (MIF) promotes cell survival by activation of the Akt pathway and role for CSN5/JAB1 in the control of autocrine MIF activity

Categories:

Transfection

Authors:

Lue H, Thiele M, Franz J, Dahl E, Speckgens S, Leng L, Fingerle-Rowson G, Bucala R, Luscher B, Bernhagen J

In:

Oncogene (2007) 26(35): 5046-59

The role of the poly(ADP-ribose) polymerase tankyrase1 in telomere length control by the TRF1 component of the shelterin complex

Categories:

Transfection

Authors:

Donigian JR, de Lange T

In:

J Biol Chem (2007) 282(31): 22662-7

The Atg5 Atg12 conjugate associates with innate antiviral immune responses

Categories:

Transfection

Authors:

Jounai N, Takeshita F, Kobiyama K, Sawano A, Miyawaki A, Xin KQ, Ishii KJ, Kawai T, Akira S, Suzuki K, Okuda K

In:

Proc Natl Acad Sci USA (2007) 104(35): 14050-5

Sequential Activation of Poly(ADP-Ribose) Polymerase 1, Calpains, and Bax Is Essential in Apoptosis-Inducing Factor-Mediated Programmed Necrosis

Categories:

Transfection

Authors:

Moubarak RS, Yuste VJ, Artus C, Bouharrour A, Greer PA, Menissier-de Murcia J, Susin SA

In:

Mol Cell Biol (2007) 27(13): 4844-62

Molecular mechanisms involved in the regulation of cytokine production by muramyl dipeptide

Categories:

Transfection

Authors:

Windheim M, Lang C, Peggie M, Cummings L, Cohen P

In:

Biochem J (2007) 404(2): 179-90

A BAX/BAK-independent mechanism of cytochrome C release

Categories:

Transfection

Authors:

Mizuta T, Shimizu S, Matsuoka Y, Nakagawa T, Tsujimoto Y

In:

J Biol Chem (2007) 282(22): 16623-16630

c-Jun N-terminal kinase mediates hydrogen peroxide-induced cell death via sustained poly(ADP-ribose) polymerase-1 activation

Categories:

Transfection

Authors:

Zhang S, Lin Y, Kim YS, Hande MP, Liu ZG, Shen HM

In:

Cell Death Differ (2007) 14(5): 1001-10

A novel TRAF6 binding site in MALT1 defines distinct mechanisms of NF-kappa B activation by API2-MALT1 fusions

Categories:

Transfection

Authors:

Noels H, van Loo G, Hagens S, Broeckx V, Beyaert R, Marynen P, Baens M

In:

J Biol Chem (2007) 282(14): 10180-9

Deficiency in the LIM-only protein Fhl2 impairs skin wound healing

Categories:

Transfection

Authors:

Wixler V, Hirner S, Muller JM, Gullotti L, Will C, Kirfel J, Gunther T, Schneider H, Bosserhoff A, Schorle H, Park J, Schule R, Buettner R

In:

J Cell Biol (2007) 177(1): 163-72

Modulation of host cell gene expression through activation of stat transcription factors by pasteurella multocida toxin

Categories:

Transfection

Authors:

Orth JH, Aktories K, Kubatzky KF

In:

J Biol Chem (2007) 282(5): 3050-7

The C-terminal products of cellular prion protein processing, C1 and C2, exert distinct influence on p53-dependent staurosporine-induced caspase-3 activation

Categories:

Transfection

Authors:

Sunyach C, Alfa Cisse M, Alves da Costa C, Vincent B, Checler F

In:

J Biol Chem (2007) 282(3): 1956-1963

NEDD4-1 Is a Proto-Oncogenic Ubiquitin Ligase for PTEN

Categories:

Transfection

Authors:

Wang X, Trotman LC, Koppie T, Alimonti A, Chen Z, Gao Z, Wang J, Erdjument-Bromage H, Tempst P, Cordon-Cardo C, Pandolfi PP, Jiang X

In:

Cell (2007) 128(1): 129-39

PARP-1 and Ku compete for repair of DNA double strand breaks by distinct NHEJ pathways

Categories:

Transfection

Authors:

Wang M, Wu W, Wu W, Rosidi B, Zhang L, Wang H, Iliakis G

In:

Nucleic Acids Res (2006) 34(21): 6170-82

The mediator complex functions as a coactivator for GATA-1 in erythropoiesis via subunit Med1/TRAP220

Categories:

Transfection

Authors:

Stumpf M, Waskow C, Krotschel M, van Essen D, Rodriguez P, Zhang X, Guyot B, Roeder RG, Borggrefe T

In:

Proc Natl Acad Sci USA (2006) 103(49): 18504-9

A time- and dose-dependent STAT1 expression system

Categories:

Transfection

Authors:

Leitner NR, Strobl B, Bokor M, Painz R, Kolbe T, Rulicke T, Muller M, Karaghiosoff M

In:

BMC Biotechnol (2006) 6: 48

Re-expression of the tumor suppressor NF2/merlin inhibits invasiveness in mesothelioma cells and negatively regulates FAK

Categories:

Transfection

Authors:

Poulikakos PI, Xiao GH, Gallagher R, Jablonski S, Jhanwar SC, Testa JR

In:

Oncogene (2006) 25(44): 5960-8

The crystal structure of human IRE1 luminal domain reveals a conserved dimerization interface required for activation of the unfolded protein response

Categories:

Transfection

Authors:

Zhou J, Liu CY, Back SH, Clark RL, Peisach D, Xu Z, Kaufman RJ

In:

Proc Natl Acad Sci USA (2006) 103(39): 14343-8

Calsenilin interacts with transcriptional co-repressor C-terminal binding protein(s)

Categories:

Transfection

Authors:

Zaidi NF, Kuplast KG, Washicosky KJ, Kajiwara Y, Buxbaum JD, Wasco W

In:

J Neurochem (2006) 98(4): 1290-301

Integrin-mediated adhesion regulates membrane order

Categories:

Transfection

Authors:

Gaus K, Le Lay S, Balasubramanian N, Schwartz MA

In:

J Cell Biol (2006) 174(5): 725-34

Cytoplasmic IRE1alpha -mediated XBP1 mRNA splicing in the absence of nuclear processing and ER stress

Categories:

Transfection

Authors:

Back SH, Lee K, Vink E, Kaufman RJ

In:

J Biol Chem (2006) 281(27): 18691-706

Regulation of antiviral responses by a direct and specific interaction between TRAF3 and Cardif

Categories:

Transfection

Authors:

Saha SK, Pietras EM, He JQ, Kang JR, Liu SY, Oganesyan G, Shahangian A, Zarnegar B, Shiba TL, Wang Y, Cheng G

In:

EMBO J (2006) 25(14): 3257-63

Specific and Nonspecific Membrane-binding Determinants Cooperate in Targeting Phosphatidylinositol Transfer Protein -Isoform to the Mammalian trans-Golgi Network

Categories:

Transfection

Authors:

Phillips SE, Ile KE, Boukhelifa M, Huijbregts RP, Bankaitis VA

In:

Mol Biol Cell (2006) 17(6): 2498-2512

CNF1-induced Ubiquitylation and Proteasome Destruction of Activated RhoA Is Impaired in Smurf1-/- Cells

Categories:

Transfection

Authors:

Boyer L, Turchi L, Desnues B, Doye A, Ponzio G, Mege JL, Yamashita M, Zhang YE, Bertoglio J, Flatau G, Boquet P, Lemichez E

In:

Mol Biol Cell (2006) 17(6): 2489-2497

Sortilin, SorCS1b, and SorLA Vps10p sorting receptors, are novel gamma-secretase substrates

Categories:

Transfection

Authors:

Nyborg AC, Ladd TB, Zwizinski CW, Lah JJ, Golde TE

In:

Mol Neurodegener (2006) 1: 3

Cyclin-dependent kinase 2 is dispensable for normal centrosome duplication but required for oncogene-induced centrosome overduplication

Categories:

Transfection

Authors:

Duensing A, Liu Y, Tseng M, Malumbres M, Barbacid M, Duensing S

In:

Oncogene (2006) 25(20): 2943-9

FAK-dependent regulation of myofibroblast differentiation

Categories:

Transfection

Authors:

Greenberg RS, Bernstein AM, Benezra M, Gelman IH, Taliana L, Masur SK

In:

FASEB J (2006) 20(7): 1006-8

A Kinase-Independent Function of c-Abl in Promoting Proteolytic Destruction of Damaged DNA Binding Proteins

Categories:

Transfection

Authors:

Chen X, Zhang J, Lee J, Lin PS, Ford JM, Zheng N, Zhou P

In:

Mol Cell (2006) 22(4): 489-99

Cell Surface Transglutaminase Promotes RhoA Activation via Integrin Clustering and Suppression of the Src-p190RhoGAP Signaling Pathway

Categories:

Transfection

Authors:

Janiak A, Zemskov EA and Belkin AM

In:

Mol Biol Cell (2006) 17(4): 1606-19

Genetic elimination of Suppressor of fused reveals an essential repressor function in the mammalian Hedgehog signaling pathway

Categories:

Transfection

Authors:

Svard J, Heby-Henricson K, Persson-Lek M, Rozell B, Lauth M, Bergstrom A, Ericson J, Toftgard R, Teglund S

In:

Dev Cell (2006) 10(2): 187-97

Rac1 Leads to Phosphorylation-dependent Increase in Stability of the p66shc Adaptor Protein: Role in Rac1-induced Oxidative Stress

Categories:

Transfection

Authors:

Khanday FA, Yamamori T, Mattagajasingh I, Zhang Z, Bugayenko A, Naqvi A, Santhanam L, Nabi N, Kasuno K, Day BW and Irani K

In:

Mol Biol Cell (2006) 17(1): 122-129

Expression of endogenous oncogenic V600EB-raf induces proliferation and developmental defects in mice and transformation of primary fibroblasts

Categories:

Transfection

Authors:

Mercer K, Giblett S, Green S, Lloyd D, Darocha Dias S, Plumb M, Marais R and Pritchard C

In:

Cancer Res (2005) 65(24): 11493-11500

Pasteurella multocida Toxin-induced Activation of RhoA Is Mediated via Two Families of G Proteins, Gq and G12/13

Categories:

Transfection

Authors:

Orth JH, Lang S, Taniguchi M and Aktories K

In:

J Biol Chem (2005) 280(44): 36701-36707

Loss of bif-1 suppresses bax/bak conformational change and mitochondrial apoptosis

Categories:

Transfection

Authors:

Takahashi Y, Karbowski M, Yamaguchi H, Kazi A, Wu J, Sebti SM, Youle RJ and Wang HG

In:

Mol Cell Biol (2005) 25(21): 9369-9382

CCM1 and CCM2 protein interactions in cell signaling: implications for cerebral cavernous malformations pathogenesis

Categories:

Transfection

Authors:

Zawistowski JS, Stalheim L, Uhlik MT, Abell AN, Ancrile BB, Johnson GL and Marchuk DA

In:

Hum Mol Genet (2005) 14(17): 2521-2531

A-Raf and Raf-1 work together to influence transient ERK phosphorylation and Gl/S cell cycle progression

Categories:

Transfection

Authors:

Mercer K, Giblett S, Oakden A, Brown J, Marais R and Pritchard C

In:

Oncogene (2005) 24(33): 5207-5217

DNA ligase III as a candidate component of backup pathways of nonhomologous end joining

Categories:

Transfection

Authors:

Wang H, Rosidi B, Perrault R, Wang M, Zhang L, Windhofer F and Iliakis G

In:

Cancer Res (2005) 65(10): 4020-4030

The LIM protein Ajuba regulates phosphatidylinositol 4,5-bisphosphate levels in migrating cells through an interaction with and activation of PIPKI

Categories:

Transfection

Authors:

Kisseleva M, Feng Y, Ward M, Song C, Anderson RA and Longmore GD

In:

Mol Cell Biol (2005) 25(10): 3956-3966

The sustainability of interactions between the orexin-1 receptor and beta-arrestin-2 is defined by a single C-terminal cluster of hydroxy amino acids and modulates the kinetics of ERK MAPK regulation

Categories:

Transfection

Authors:

Milasta S, Evans NA, Ormiston L, Wilson S, Lefkowitz RJ, Milligan G

In:

Biochem J (2005) 387(Pt 3): 573-84

beta-arrestin Dependent Spontaneous 1a-Adrenoceptor Endocytosis causes Intracellular Transportation of -blockers via Recycling Compartments

Categories:

Transfection

Authors:

Pediani JD, Colston JF, Caldwell D, Milligan G, Daly CJ, McGrath JC

In:

Mol Pharmacol (2005) 67(4): 992-1004

ADAR1 RNA deaminase limits siRNA efficacy in mammalian cells

Categories:

Transfection

Authors:

Yang W, Wang Q, Howell KL, Lee JT, Cho DS, Murray JM and Nishikura K

In:

J Biol Chem (2005) 280(5): 3946-3953

The LIM protein Ajuba influences p130Cas localization and Rac1 activity during cell migration

Categories:

Transfection

Authors:

Pratt SJ, Epple H, Ward M, Feng Y, Braga VM and Longmore GD

In:

J Cell Biol (2005) 168(5): 813-824

Quantitative analysis of translesion DNA synthesis across a benzo[a]pyrene-guanine adduct in mammalian cells: The role of DNA polymerase k

Categories:

Transfection

Authors:

Avkin S, Goldsmith M, Velasco-Miguel S, Geacintov N, Friedberg EC and Livneh Z

In:

J Biol Chem (2004) 279(51): 53298-53305

Fzo1, a protein involved in mitochondrial fusion, inhibits apoptosis

Categories:

Transfection

Authors:

Sugioka R, Shimizu S and Tsujimoto Y

In:

J Biol Chem (2004) 279(50): 52726-52734

Role of Bcl-2 family proteins in a non-apoptotic programmed cell death dependent on autophagy genes

Categories:

Transfection

Authors:

Shimizu S, Kanaseki T, Mizushima N, Mizuta T, Arakawa-Kobayashi S, Thompson CB, Tsujimoto Y

In:

Nat Cell Biol (2004) 6(12): 1221-1228

PIASy-deficient mice display modest defects in IFN and Wnt signaling

Categories:

Transfection

Authors:

Roth W, Sustmann C, Kieslinger M, Gilmozzi A, Irmer D, Kremmer E, Turck C and Grosschedl R

In:

J Immunol (2004) 173(10): 6189-6199

A caspase-8-independent signaling pathway activated by Fas ligation leads to exposure of the Bak N terminus

Categories:

Transfection

Authors:

Zhang L, Shimizu S, Sakamaki K, Yonehara S and Tsujimoto Y

In:

J Biol Chem (2004) 279(32): 33865-33874

PKD1/PKCµ promotes alphavbeta3 integrin recycling and delivery to nascent focal adhesions

Categories:

Transfection

Authors:

Woods AJ, White DP, Caswell PT and Norman JC

In:

EMBO J (2004) 23(13): 2531-2543

B-Raf acts via the ROCKII/LIMK/cofilin pathway to maintain actin stress fibers in fibroblasts

Categories:

Transfection

Authors:

Pritchard CA, Hayes L, Wojnowski L, Zimmer A, Marais RM and Norman JC

In:

Mol Cell Biol (2004) 24(13): 5937-5952

Nonmuscle Myosin IIB is involved in the guidance of fibroblast migration

Categories:

Transfection

Authors:

Lo CM, Buxton DB, Chua GCH, Dembo M, Adelstein RS and Wang YL

In:

Mol Biol Cell (2004) 15(3): 982-989

A central role for the Jun-N-terminal kinase pathway in mediating the antagonistic activity of pro-inflammatory cytokines against transforming growth factor-beta-driven Smad3/4 specific gene expression

Categories:

Transfection

Authors:

Verrecchia F, Tacheau C, Wagner EF and Mauviel A

In:

J Biol Chem (2003) 278: 1585-1593

Distinct involvement of the Jun-N-terminal kinase and NF-kappaB pathways in the expression of the human COL1A2 gene by TNF-alpha

Categories:

Transfection

Authors:

Verrecchia F, Wagner EF and Mauviel A

In:

EMBO Rep (2002) 3(11): 1069-1074

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Embryonic fibroblast, mouse (MEF) immort

Transfection Information

Citations (72)