Neuron, cortical, mouse

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Neurons isolated from the cerebral cortex.

Cell Type:
Neurons (Brain)
Tissue Origin:
brain
Species:
mouse
Research Area:
Neurobiology
Cell Characteristics:
Adherent

Recommended Media

Primary Neuron Growth Medium

Primary Neuron Growth Medium Kit (PNGM™) was specifically developed for culturing embryonic primary rat and mouse neurons. This serum-free medium allows for long-term maintenance and growth of neuronal cells, and maintains pure populations of neuronal cells without the need of an astrocyte feeder layer.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Neuron, mouse O-005 92% ±2 Plasmid (general) 100 µl I/II/2b
AD1 ER-137 2e5 30-50% 50-80% Plasmid (general) 17.5 µg 350 µl 4D Y-Unit

Citations (30)

Cell Assembly Signatures Defined by Short-Term Synaptic Plasticity in Cortical Networks.
Categories:
Primary Cells and Media 
Authors:
Carrillo-Reid L, Lopez-Huerta VG, Garcia-Munoz M, Theiss S, Arbuthnott GW 
In:
Other (2015) 25(7): ePub 
The Cannabinoid Receptor CB1 Interacts with the WAVE1 Complex and Plays a Role in Actin Dynamics and Structural Plasticity in Neurons.
Categories:
Transfection 
Authors:
Christian Njoo, Nitin Agarwal, Beat Lutz, Rohini Kuner 
In:
PLoS Biol (2015) 13(10): e1002286 
Homozygous SLC6A17 mutations cause autosomal-recessive intellectual disability with progressive tremor, speech impairment, and behavioral problems.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Iqbal Z1, Willemsen MH1, Papon MA2, Musante L3, Benevento M4, Hu H3, Venselaar H5, Wissink-Lindhout WM1, Vulto-van Silfhout AT1, Vissers LE1, de Brouwer AP6, Marouillat S2, Wienker TF3, Ropers HH3, Kahrizi K7, Nadif Kasri N6, Najmabadi H7, Laumonnier F8, Kleefstra T1, van Bokhoven H9. 
In:
Am J Hum Genet (2015) 96(3): 386-396 
Extrasynaptic glutamate NMDA receptors: Key players in striatal function.
Categories:
Primary Cells and Media 
Authors:
Garcia-Munoz M, Lopez-Huerta VG, Carrillo-Reid L, Arbuthnott GW 
In:
Other (2015) 89: 54-63 
Amiodarone biokinetics, the formation of its major oxidative metabolite and neurotoxicity after acute and repeated exposure of brain cell cultures.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Pomponio G, Zurich MG, Schultz L, Weiss DG, Romanelli L, Gramowski-Voss A, Di Consiglio E, Testai E. 
In:
Toxicol In Vitro (2015) (15)00013-2: 1 
The enhancer landscape during early neocortical development reveals patterns of dense regulation and co-option
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Wenger AM, Clarke SL, Notwell JH, Chung T, Tuteja G, Guturu H, Schaar BT, Bejerano G. 
In:
PLoS Genet (2013) 9(8): 1-14 
Dynamic interaction between P-bodies and transport ribonucleoprotein particles in dendrites of mature hippocampal neurons
Categories:
Transfection 
Authors:
Zeitelhofer M, Karra D, Macchi P, Tolino M, Thomas S, Schwarz M, Kiebler M, Dahm R 
In:
J Neurosci (2008) 28(30): 7555-62 
Protein 600 is a microtubule/endoplasmic reticulum-associated protein in CNS neurons
Categories:
Transfection 
Authors:
Shim SY, Wang J, Asada N, Neumayer G, Tran HC, Ishiguro K, Sanada K, Nakatani Y, Nguyen MD 
In:
J Neurosci (2008) 28(14): 3604-14 
Endophilin I expression is increased in the brains of Alzheimer's disease patients
Categories:
Transfection 
Authors:
Ren Y, Xu HW, Davey F, Taylor M, Aiton J, Coote P, Fang F, Yao J, Chen JX, Yan SD, Gunn-Moore FJ 
In:
J Biol Chem (2008) 283(9): 5685-91 
NCAM is ubiquitylated, endocytosed and recycled in neurons
Categories:
Transfection 
Authors:
Diestel S, Schaefer D, Cremer H, Schmitz B 
In:
J Cell Sci (2007) 120(Pt 22): 4035-49 
Sez-6 proteins affect dendritic arborization patterns and excitability of cortical pyramidal neurons
Categories:
Transfection 
Authors:
Gunnersen JM, Kim MH, Fuller SJ, De Silva M, Britto JM, Hammond VE, Davies PJ, Petrou S, Faber ES, Sah P, Tan SS 
In:
Neuron (2007) 56(4): 621-39 
Refined Characterization of the Expression and Stability of the SMN Gene Products
Categories:
Transfection 
Authors:
Vitte J, Fassier C, Tiziano FD, Dalard C, Soave S, Roblot N, Brahe C, Saugier-Veber P, Bonnefont JP, Melki J 
In:
Am J Pathol (2007) 171(4): 1269-80 
An essential switch in subunit composition of a chromatin remodeling complex during neural development
Categories:
Transfection 
Authors:
Lessard J, Wu JI, Ranish JA, Wan M, Winslow MM, Staahl BT, Wu H, Aebersold R, Graef IA, Crabtree GR 
In:
Neuron (2007) 55(2): 201-15 
Spinophilin facilitates dephosphorylation of doublecortin by PP1 to mediate microtubule bundling at the axonal wrist
Categories:
Transfection 
Authors:
Bielas SL, Serneo FF, Chechlacz M, Deerinck TJ, Perkins GA, Allen PB, Ellisman MH, Gleeson JG 
In:
Cell (2007) 129(3): 579-91 
BAD-LAMP defines a subset of early endocytic organelles in subpopulations of cortical projection neurons
Categories:
Transfection 
Authors:
David A, Tiveron MC, Defays A, Beclin C, Camosseto V, Gatti E, Cremer H, Pierre P 
In:
J Cell Sci (2007) 120(Pt 2): 353-65 
p130(CAS) is required for netrin signaling and commissural axon guidance
Categories:
Transfection 
Authors:
Liu G, Li W, Gao X, Li X, Jurgensen C, Park HT, Shin NY, Yu J, He ML, Hanks SK, Wu JY, Guan KL, Rao Y 
In:
J Neurosci (2007) 27(4): 957-68 
A mutation of spastin is responsible for swellings and impairment of transport in a region of axon characterized by changes in microtubule composition
Categories:
Transfection 
Authors:
Tarrade A, Fassier C, Courageot S, Charvin D, Vitte J, Peris L, Thorel A, Mouisel E, Fonknechten N, Roblot N, Seilhean D, Dierich A, Hauw JJ, Melki J 
In:
Hum Mol Genet (2006) 15(24): 3544-58 
Cdk5 phosphorylates and stabilizes p27(kip1) contributing to actin organization and cortical neuronal migration
Categories:
Transfection 
Authors:
Kawauchi T, Chihama K, Nabeshima Y and Hoshino M 
In:
Nat Cell Biol (2006) 8(1): 17-26 
Multiple Eph receptors and B-class ephrins regulate midline crossing of corpus callosum fibers in the developing mouse forebrain
Categories:
Transfection 
Authors:
Mendes SW, Henkemeyer M and Liebl DJ 
In:
J Neurosci (2006) 26(3): 882-892 
A schizophrenia-associated mutation of DISC1 perturbs cerebral cortex development
Categories:
Transfection 
Authors:
Kamiya A, Kubo K, Tomoda T, Takaki M, Youn R, Ozeki Y, Sawamura N, Park U, Kudo C, Okawa M, Ross CA, Hatten ME, Nakajima K and Sawa A 
In:
Nat Cell Biol (2005) 7(12): 1067-1078 
Leucine-rich repeat kinase 2 (LRRK2) interacts with parkin, and mutant LRRK2 induces neuronal degeneration
Categories:
Transfection 
Authors:
Smith WW, Pei Z, Jiang H, Moore DJ, Liang Y, West AB, Dawson VL, Dawson TM, Ross CA 
In:
Proc Natl Acad Sci USA (2005) 102(51): 18676-18681 
Both the phosphoinositide and receptor binding activities of Dab1 are required for Reelin-stimulated Dab1 tyrosine phosphorylation
Categories:
Transfection 
Authors:
Xu M, Arnaud L, Cooper JA 
In:
Brain Res Mol Brain Res (2005) 139(2): 300-5 
Vesicle-mediated transport and release of CCL21 in endangered neurons: a possible explanation for microglia activation remote from a primary lesion
Categories:
Transfection 
Authors:
de Jong EK, Dijkstra IM, Hensens M, Brouwer N, van Amerongen M, Liem RS, Boddeke HW and Biber K 
In:
J Neurosci (2005) 25(33): 7548-7557 
Tissue inhibitor of metalloproteinase-2 promotes neuronal differentiation by acting as an anti-mitogenic signal
Categories:
Transfection 
Authors:
Perez-Martinez L and Jaworski DM 
In:
J Neurosci (2005) 25(20): 4917-4929 
Involvement of a Rac activator,P-Rex1, in neurotrophin-derived signaling and neuronal migration
Categories:
Transfection 
Authors:
Yoshizawa M, Kawauchi T, Sone M, Nishimura YV, Terao M, Chihama K, Nabeshima Y and Hoshino M 
In:
J Neurosci (2005) 25(17): 4406-4419 
A structure-based analysis of huntingtin mutant polyglutamine aggregation and toxicity: evidence for a compact beta-sheet structure
Categories:
Transfection 
Authors:
Poirier MA, Jiang H and Ross CA 
In:
Hum Mol Genet (2005) 14(6): 765-774 
Regulation of miRNA expression during neural cell specification
Categories:
Transfection 
Authors:
Smirnova L, Grafe A, Seiler A, Schumacher S, Nitsch R and Wulczyn FG 
In:
Eur J Neurosci (2005) 21(6): 1469-1477 
alpha3beta1 integrin modulates neuronal migration and placement during early stages of cerebral cortical development
Categories:
Transfection 
Authors:
Schmid RS, Shelton S, Stanco A, Yokota Y, Kreidberg JA and Anton ES 
In:
Development (2004) 131(24): 6023-6031 
Ndel1 Operates in a Common Pathway with LIS1 and Cytoplasmic Dynein to Regulate Cortical Neuronal Positioning
Categories:
Transfection 
Authors:
Shu T, Ayala R, Nguyen MD, Xie Z, Gleeson JG and Tsai LH 
In:
Neuron (2004) 44(2): 263-277 
A NUDEL-dependent mechanism of neurofilament assembly regulates the integrity of CNS neurons
Categories:
Transfection 
Authors:
Nguyen MD, Shu T, Sanada K, Lariviere RC, Tseng HC, Park SK, Julien JP and Tsai LH 
In:
Nat Cell Biol (2004) 6(7): 595-608 
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