NIH/3T3

Swiss NIH embryonic fibroblast

Cell Type:
Fibroblast
Tissue Origin:
embryo
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent

Recommended Media

PC-1™ is a low-protein, serum-free medium intended for the culture of primary cells and anchorage-dependent cell lines. PC-1 is formulated in a specially modified DMEM/F12 base and contains a complete HEPES buffering system with known amounts of insulin, transferrin, fatty acids, and proprietary proteins assembled under strict quality control procedures. PC-1™ is intended for a variety of research and industrial applications and is formulated using defined components for optimal cell growth, while maintaining the lowest possible protein content.
PC-1™ does not contain L-glutamine.

PC-1™ Liquid Base Medium is to be stored at 2°C-8°C.
The Supplement should be stored at -20°C.
When these two components are combined, the resulting PC-1™ Complete Medium is stable for 45 days at 2°C-8°C.

Once thawed, the appropriate volume of one vial of PC-1™ Supplement must be combined with the companion volume of PC-1™ Liquid Base Medium. Partial reconstitution or repeated freezing and thawing of the PC-1™ Supplement is not advised.

UltraMEM Reduced Serum Medium is a chemically-defined medium designed to support growth and maintenance of several anchorage-dependent cell types under reduced serum concentrations. When supplemented with 2-4% serum, UltraMEM Medium growth performance is comparable and, in some instances, superior to that of standard media supplemented with 10% fetal bovine serum.

Growth performance in UltraMEM can be further increased by addition of insulin, transferrin, selenium, and ethanolamine [ITS or ITES] to the basal medium.

Storage = 2ºC to 8ºC 

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
R A-024 1e6 68% ±17 89% Plasmid (general) 1 µg 100 µl I/II/2b
SG EN-158 2e5 90% ±2 51% ±5 Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SG EN-158 1e6 88.1% ±2 53% ±3 Plasmid (general) 2 µg 100 µl 4D X-Unit
SG 96-EN-158 2e5 95% ±2 93% ±4 Plasmid (general) 0.4 µg 20 µl Shuttle
R U-030 1e6 84% ±4 87% Plasmid (general) 1 µg 100 µl I/II/2b

Citations (38)

Categories:
3D Cell Culture , Transfection 
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SLAS Discov (2019) 1: 12 
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Transfection 
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In:
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Categories:
Transfection 
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In:
Genome Res (2010) 186(2): 451-9 
Categories:
Transfection 
Authors:
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In:
Nat Cell Biol (2008) 10(5): 556-66 
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Transfection 
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Categories:
Transfection 
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Categories:
Transfection 
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Categories:
Transfection 
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Categories:
Transfection 
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Categories:
Transfection 
Authors:
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Categories:
Transfection 
Authors:
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Transfection 
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Categories:
Transfection 
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Categories:
Transfection 
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Cancer Cell (2006) 10(5): 425-35 
Categories:
Transfection 
Authors:
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In:
EMBO J (2006) 25(20): 4952-62 
Categories:
Transfection 
Authors:
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In:
J Biol Chem (2006) 281(29): 20055-67 
Categories:
Transfection 
Authors:
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Blood (2006) 107(10): 4130-8 
Categories:
Transfection 
Authors:
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Biophys J (2006) 90(10): 3774-82 
Categories:
Transfection 
Authors:
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In:
J Biol Chem (2006) 281(9): 5718-25 
Categories:
Transfection 
Authors:
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In:
Oncogene (2006) 25(6): 940-53 
Categories:
Transfection 
Authors:
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Transfection 
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Transfection 
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Categories:
Transfection 
Authors:
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Categories:
Transfection 
Authors:
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Mol Cell Biol (2005) 25(7): 2632-2643 
Categories:
Transfection 
Authors:
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Oncogene (2005) 24(16): 2739-2744 
Categories:
Transfection 
Authors:
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In:
Exp Dermatol (2005) 14(3): 209-214 
Categories:
Transfection 
Authors:
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Oncogene (2005) 24(7): 1272-1276 
Categories:
Transfection 
Authors:
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In:
J Cell Sci (2005) 118(Pt 1): 101-112 
Categories:
Transfection 
Authors:
Venugopal J, Hanashiro K, Yang ZZ and Nagamine Y 
In:
Proc Natl Acad Sci USA (2004) 101(49): 17120-17125 
Categories:
Transfection 
Authors:
Beningo KA, Dembo M and Wang YL 
In:
Proc Natl Acad Sci USA (2004) 101(52): 18024-18029 
Categories:
Transfection 
Authors:
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Arch Dermatol Res (2004) 296(2): 59-66 
Categories:
Transfection 
Authors:
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Categories:
Transfection 
Authors:
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In:
Mol Cell Biol (2004) 24(4): 1505-1515 
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