Apoptosis of the dermal papilla cells of hair follicle associated with the expression of gene HSPCO16 in vitro

Yang WB, Hao F, Song ZQ, Yang XC, Ni B
Source: Exp Dermatol
Publication Date: (2005)
Issue: 14(3): 209-214
Research Area:
Dermatology/Tissue Engineering
Cells used in publication:
Species: mouse
Tissue Origin: embryo
Nucleofectorâ„¢ I/II/2b
Used nucleofection to overexpress HSPC016 in NIH 3T3 and primary human dermal papilla cells in order to examine effect on apoptosis
The epithelial-mesenchymal interactions have an important role in the folliculomorphogenesis and mature hair follicle cycling. The dermal papilla, a dense aggregate of specialized dermis-derived stromal cells located at the bottom of the hair follicle, is a major component of hair, which signals the follicular epithelial cells to prolong the hair growth process. However, to date, little is known about the significance of the specific gene(s) expression in the dermal papilla cells with regard to their aggregative behaviour and hair cycling. In our previous study, the differentially gene-expressed cDNAs library had been determined by means of suppression subtractive hybridization technique between the aggregated human dermal papilla cells and control cells. Among those cDNAs library, the haematopoietic stem/progenitor cell (HSPC)-related gene HSPC016 was found. In this study, the gene HSPC016 was confirmed to express in the human dermal papilla cells by means of in situ hybridization and reverse transcriptase-polymerase chain reaction. In order to rudimentarily elucidate its biological function, a recombinant eucaryotic expressing plasmid pcDNA3.1(+)/HSPC016 was constructed and was transfected into the human dermal papilla cells and 3T3 fibroblast cells by means of Nucleofector(TM) technique (Amaxa, Cologne, Germany). Terminal deoxynucleotidyl transferase-mediated d-UTP nick end Labelling (TUNEL) assay showed that the expression of gene HSPCO16 resulted in the apoptosis of these cells, which suggested that the apoptosis of dermal papilla cells might be associated with the expression of gene HSPC016 in vitro.