cells

(also called MCF-7) human caucasian mammary gland adenocarcinoma

Cell Type:

Mammary Epith.

Tissue Origin:

breast

Species:

human

Research Area:

Cancer Research/Cell Biology

Cell Characteristics:

Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
SE	EN-130	4e5	78.2% ±1	52-66%	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
SE	EN-130	2e6	78.4% ±5	52-66%	Plasmid (general)	2 µg	100 µl	4D X-Unit
V	P-020	2e6	71% ±4	60%	Plasmid (general)	2 µg	100 µl	I/II/2b
SE	96-EN-130	4e5	72% ±3	59% ±7	Plasmid (general)	0.4 µg	20 µl	96-well

Citations (31)

Migration of breast cancer cell lines in response to pulmonary laminin 332.

Categories:

Primary Cells and Media

Authors:

Carpenter PM, Sivadas P, Hua SS, Xiao C, Gutierrez AB, Ngo T, Gershon PD

In:

Cancer Medicine (2017) 6(1): 220-234

6-Shogaol Inhibits Breast Cancer Cells and Stem Cell-Like Spheroids by Modulation of Notch Signaling Pathway and Induction of Autophagic Cell Death

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Ray A, Vasudevan S, Sengupta S

In:

PLoS ONE (2015) 10(9): e0137614

Koenimbin, a natural dietary compound of Murraya koenigii (L) Spreng: inhibition of MCF7 breast cancer cells and targeting of derived MCF7 breast cancer stem cells (CD44(+)/CD24(-/low)): an in vitro study

Categories:

Primary Cells and Media

Authors:

Ahmadipour F, Noordin MI, Mohan S, Arya A, Paydar M, Looi CY, Keong YS, Siyamak EN, Fani S, Firoozi M, Yong CL1, Sukari MA, Kamalidehghan B

In:

Other (2015) 9: 1193-208

Targeting MKK3 as a novel anticancer strategy: molecular mechanisms and therapeutical implications

Categories:

Primary Cells and Media

Authors:

Baldari S, Ubertini V, Garufi A, D'Orazi G, Bossi G

In:

Cell Death Dis. (2015) 6: e1621

Cooperation between BRCA1 and vitamin D is critical for histone acetylation of the p21waf1 promoter and for growth inhibition of breast cancer cells and cancer stem-like cells.

Categories:

Authors:

Pickholtz I, Saadyan S, Keshet GI, Wang VS, Cohen R, Bouwman P, Jonkers J, Byers SW6, Papa MZ, Yarden RI.

In:

OncoTarget (2014) 2(23): 11827-46

High-efficiency genome editing via 2A-coupled co-expression of fluorescent proteins and zinc finger nucleases or CRISPR/Cas9 nickase pairs.

Categories:

Transfection

Authors:

Duda K, Lonowski LA, Kofoed-Nielsen M, Ibarra A, Delay CM, Kang Q, Yang Z, Pruett-Miller SM, Bennett EP3, Wandall HH, Davis GD, Hansen SH, Frödin M.

In:

Nucleic Acids Res (2014) 42: 1-16

STAT5b, c-Src, and EGFR Signaling Play Integral Roles in Estrogen-Stimulated Proliferation of ER-Positive Breast Cancer Cells

Categories:

Transfection

Authors:

Fox EM, Bernaciak TM, Wen J, Weaver AM, Shupnik MA, Silva CM

In:

Mol Endocrinol (2008) 22(8): 1781-96

miR-206 Expression is down-regulated in estrogen receptor alpha-positive human breast cancer

Categories:

Transfection

Authors:

Kondo N, Toyama T, Sugiura H, Fujii Y, Yamashita H

In:

Cancer Res (2008) 68(13): 5004-8

Slit-2 induces a tumor suppressive effect by regulating beta -catenin in breast cancer cells

Categories:

Transfection

Authors:

Prasad A, Paruchuri V, Preet A, Latif F, Ganju RK

In:

J Biol Chem (2008) epub: online

Inflammatory signaling and aryl hydrocarbon receptor mediate synergistic induction of interleukin 6 in MCF-7 cells

Categories:

Transfection

Authors:

Hollingshead BD, Beischlag TV, Dinatale BC, Ramadoss P, Perdew GH

In:

Cancer Res (2008) 68(10): 3609-17

Selective inhibition of histone deacetylase 2 silences progesterone receptor-mediated signaling

Categories:

Transfection

Authors:

Bicaku E, Marchion DC, Schmitt ML, Münster PN

In:

Cancer Res (2008) 68(5): 1513-9

Apoptotic cell-derived sphingosine-1-phosphate promotes HuR-dependent cyclooxygenase-2 mRNA stabilization and protein expression

Categories:

Transfection

Authors:

Johann AM, Weigert A, Eberhardt W, Kuhn AM, Barra V, von Knethen A, Pfeilschifter JM, Brüne B

In:

J Immunol (2008) 180(2): 1239-48

Nerve growth factor receptor TrkA signaling in breast cancer cells involves KU70 to prevent apoptosis

Categories:

Transfection

Authors:

Com E, Lagadec C, Page A, El Yazidi-Belkoura I, Slomianny C, Spencer A, Hammache D, Rudkin BB, Hondermarck H

In:

Mol Cell Proteomics (2007) 6(11): 1842-1854

Tumor cell apoptosis polarizes macrophages role of sphingosine-1-phosphate

Categories:

Transfection

Authors:

Weigert A, Tzieply N, von Knethen A, Johann AM, Schmidt H, Geisslinger G, Brüne B

In:

Mol Biol Cell (2007) 18(10): 3810-9

PX-RICS, a novel splicing variant of RICS, is a main isoform expressed during neural development

Categories:

Transfection

Authors:

Hayashi T, Okabe T, Nasu-Nishimura Y, Sakaue F, Ohwada S, Matsuura K, Akiyama T, Nakamura T

In:

Genes Cells (2007) 12(8): 929-39

Modulation of estrogen receptor alpha protein level and survival function by DBC-1

Categories:

Transfection

Authors:

Trauernicht AM, Kim SJ, Kim NH, Boyer TG

In:

Mol Endocrinol (2007) 21(7): 1526-36

Breast cancer antiestrogen resistance-3 expression regulates breast cancer cell migration through promotion of p130Cas membrane localization and membrane ruffling

Categories:

Transfection

Authors:

Schrecengost RS, Riggins RB, Thomas KS, Guerrero MS, Bouton AH

In:

Cancer Res (2007) 67(13): 6174-82

ADAM-17 expression in breast cancer correlates with variables of tumor progression

Categories:

Transfection

Authors:

McGowan PM, Ryan BM, Hill AD, McDermott E, O'higgins N, Duffy MJ

In:

Clin Cancer Res (2007) epub: online

Regulation of RKIP binding to the N-region of the Raf-1 kinase

Categories:

Transfection

Authors:

Park S, Rath O, Beach S, Xiang X, Kelly SM, Luo Z, Kolch W, Yeung KC

In:

FEBS Lett (2006) 580(27): 6405-12

ADAM28 Is Overexpressed in Human Breast Carcinomas: Implications for Carcinoma Cell Proliferation through Cleavage of Insulin-like Growth Factor Binding Protein-3

Categories:

Transfection

Authors:

Mitsui Y, Mochizuki S, Kodama T, Shimoda M, Ohtsuka T, Shiomi T, Chijiiwa M, Ikeda T, Kitajima M, Okada Y

In:

Cancer Res (2006) 66(20): 9913-20

The histidine triad protein Hint1 triggers apoptosis independent of its enzymatic activity

Categories:

Transfection

Authors:

Weiske J, Huber O

In:

J Biol Chem (2006) 281(37): 27356-66

Regulation of the Raf-MEK-ERK pathway by protein phosphatase 5

Categories:

Transfection

Authors:

von Kriegsheim A, Pitt A, Grindlay GJ, Kolch W, Dhillon AS

In:

Nat Cell Biol (2006) 8(9): 1011-6

Polypyrimidine Tract Binding Protein Regulates IRES-Mediated Gene Expression during Apoptosis

Categories:

Transfection

Authors:

Bushell M, Stoneley M, Kong YW, Hamilton TL, Spriggs KA, Dobbyn HC, Qin X, Sarnow P, Willis AE

In:

Mol Cell (2006) 23(3): 401-12

Requirement of clathrin heavy chain for p53-mediated transcription

Categories:

Transfection

Authors:

Enari M, Ohmori K, Kitabayashi I, Taya Y

In:

Genes Dev (2006) 20(9): 1087-99

Association between Pak1 expression and subcellular localization and tamoxifen resistance in breast cancer patients

Categories:

Transfection

Authors:

Holm C, Rayala S, Jirstrom K, Stal O, Kumar R, Landberg G

In:

J Natl Cancer Inst (2006) 98(10): 671-80

Tumor invasion in the absence of epithelial-mesenchymal transition: podoplanin-mediated remodeling of the actin cytoskeleton

Categories:

Transfection

Authors:

Wicki A, Lehembre F, Wick N, Hantusch B, Kerjaschki D, Christofori G

In:

Cancer Cell (2006) 9(4): 261-72

Interferon-inducible protein IFIXalpha1 functions as a negative regulator of HDM2

Categories:

Transfection

Authors:

Ding Y, Lee JF, Lu H, Lee MH, Yan DH

In:

Mol Cell Biol (2006) 26(5): 1979-96

Reduction of Raf-1 kinase inhibitor protein expression correlates with breast cancer metastasis

Categories:

Transfection

Authors:

Hagan S, Al-Mulla F, Mallon E, Oien K, Ferrier R, Gusterson B, Garcia JJ and Kolch W

In:

Clin Cancer Res (2005) 11(20): 7392-7397

IQGAP1 Is a Scaffold for Mitogen-Activated Protein Kinase Signaling

Categories:

Transfection

Authors:

Roy M, Li Z and Sacks DB

In:

Mol Cell Biol (2005) 25(18): 7940-7952

The BRG1- and hBRM-Associated Factor BAF57 Induces Apoptosis by Stimulating Expression of the Cylindromatosis Tumor Suppressor Gene

Categories:

Transfection

Authors:

Wang L, Baiocchi RA, Pal S, Mosialos G, Caligiuri M and Sif S

In:

Mol Cell Biol (2005) 25(18): 7953-7965

Identification of RAI3 as a therapeutic target for breast cancer

Categories:

Transfection

Authors:

Nagahata T, Sato T, Tomura A, Onda M, Nishikawa K and Emi M

In:

Endocr Relat Cancer (2005) 12(1): 65-73

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