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When would be the optimal time to perform experiments post osteogenic differentiation for human mesenchymal stem cells?

Once the cells have differentiated and started laying down bone matrix, we recommend assaying the cells immediately. If cells begin to delaminate (peel of the culture plate in layers), this would indicate that the cells are embedded in the calcium...
Missing: mycoalert

How long can Lonzas rat cardiac myocytes survive in culture?

...are a non-proliferating cell type and beating in syncytium will occur within the first 48 hours after plating. Our general suggestion is to begin experimentation immediately after beating in syncytium is observed....
Missing: mycoalert

Can I use the ToxiLight™ Non-Destructive Cytotoxicity BioAssay Kit to assess the effect of transfection procedures on the health of my cells?

Yes. Just by taking a sample of the culture medium (usually 20µl) into a fresh luminometer plate and assaying it using the ToxiLight™ Non-Destructive Cytotoxicity BioAssay Kit you can see if increased levels of Adenylate...
Missing: mycoalert

Which pipet tips can be used with the 4D-Nucleofector® 96-well Unit?

Please make sure that the tips you are using can reach the bottom of the 96-well Nucleocuvette® plate to make sure that the complete sample can be aspirated after nucleofection. Compatible tips are e.g. epT.I.P.S™ (Eppendorf, Hamburg, Germany...
Missing: mycoalert

Which pipet tips can be used with the 384-well HT Nucleofector System?

Please make sure that the tips you are using can reach the bottom of the 384-well Nucleocuvette® plate to make sure that the complete sample can be aspirated after nucleofection. Compatible tips are e.g. epT.I.P.S™ (Eppendorf, Hamburg, Germany...
Missing: mycoalert

What is the difference between each of the types of HUVECs that Lonza offers?

...plating efficiency, markers, etc) in addition to the additional screening tests.Do you intend to use the cells in a high-throughput assay? Lonza offers HUVECs which have been pre-expanded and cryopreserved in high volumes making them appropriate for high...
Missing: mycoalert

Do I need to use a gelatin, fibronectin, collagen, or Matrigel™ coating when culturing my Clonetics™ Cells?

Most Clonetics™ Cells do not require an extracellular matrix when grown on tissue culture treated plastic – flasks, dishes, well plates (with the exception of hepatocytes, and the rat/mouse neuronal and dorsal root ganglion neurons, rat cardiac...
Missing: mycoalert

What is the recommended culturing protocol for Lonza's bone marrow stromal cells (2M-302)?

Lonza's bone marrow stromal cells should be thawed according to Lonza's "Procedure for thawing Poietics™ Cells". Upon thaw, cells should be plated at 3,000-4,000 cells per cm² in MyeloCult™ H5100 from StemCell Technologies, Inc. An adherent...
Missing: mycoalert

What components make up the PyroTec™ PRO robotic solution?

...readers and a Pyrowave Reader. includes Qinstruments HeatPlate incubators, positioning plates, tip rack holders, and racks for reagents, samples, dilution tubes and reagent troughs. PyroTec™ PRO is integrated with WinKQCL™ for data analysis...
Missing: mycoalert

Is there any problem using the Vialight™ Plus Kit due to the presence of immobilized antibodies on the bottom of the microplate?

There should be no problem with the use of the ViaLight™ Plus Kit with immobilized antibodies. You can if you wish confirm this with your own experiment by conducting a control experiment as follows:Coat triplicate wells of a 96 well plate with each...
Missing: mycoalert
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