Search Results

Automated production of gene-modified chimeric antigen receptor T cells using the Cocoon Platform

Autologous cell-based therapeutics have gained increasing attention in recent years because of their efficacy at treating diseases with limited therapeutic options. Chimeric antigen receptor (CAR) T-cell therapy has demonstrated clinical success in...

Authors: Trainor N, Purpura KA, Middleton K, Fargo K, Hails L, Vicentini-Hogan M, McRobie C, Daniels R,...

SST Peer Reviewed

Modulating mutational outcomes and improving precise gene editing at CRISPR-Cas9-induced breaks by chemical inhibition of end-joining pathways

Gene editing through repair of CRISPR-Cas9-induced chromosomal breaks offers a means to correct a wide range of genetic defects. Directing repair to produce desirable outcomes by modulating DNA repair pathways holds considerable promise to increase...

Authors: Schimmel J, Muñoz-Subirana N, Kool H, van Schendel R, van der Vlies S, Kamp JA, de Vrij FMS, Kushner...

SST Peer Reviewed

Molecular and Physiological Effects of Browning Agents on White Adipocytes from Bone Marrow Mesenchymal Stromal Cells

Two different types of adipose depots can be observed in mammals: white adipose tissue (WAT) and brown adipose tissue (BAT). The primary role of WAT is to deposit surplus energy in the form of triglycerides, along with many metabolic and hormonal...

Authors: Girolamo Di Maio, Nicola Alessio, Gianfranco Peluso, Silverio Perrotta, Marcellino Monda, Giovanni...

Automation, Monitoring, and Standardization of Cell Product Manufacturing

Although regenerative medicine products are at the forefront of scientific research, technological innovation, and clinical translation, their reproducibility and large-scale production are compromised by automation, monitoring, and standardization...

Authors: Meletios-Nikolaos Doulgkeroglou 1 2, Alessia Di Nubila 1 2, Bastian Niessing 3, Niels...

SST Peer Reviewed

Protocol to create phenotypic primary human hepatocyte cultures using the RASTRUM 3D cell model platform

To improve human hepatotoxicity prediction, in vitro liver cell models replicating hepatocyte function, drug metabolism, and toxicity are required. Here, we present a protocol for creating 3D primary human hepatocyte (PHH) cell models using the...

Authors: Christine Yee , Yik Lung Chan , Robert Utama , Marie Besnier , Martin Engel , Lisa Belfiore

A reference human induced pluripotent stem cell line for large-scale collaborative studies

Human induced pluripotent stem cell (iPSC) lines are a powerful tool for studying development and disease, but the considerable phenotypic variation between lines makes it challenging to replicate key findings and integrate data across research...

Authors: Pantazis CB, Yang A, Lara E, McDonough JA, Blauwendraat C, Peng L, Oguro H, Kanaujiya J, Zou J,...

SST Peer Reviewed

A High-Throughput Microphysiological Liver Chip System to Model Drug-Induced Liver Injury Using Human Liver Organoids

Background and AimsDrug-induced liver injury (DILI) is a major failure mode in pharmaceutical development. This study aims to address the limitations of existing preclinical models by assessing a high-throughput, microfluidic liver-on-a-chip system,...

Authors: Sophia R. Meyer , Charles J. Zhang , Max A. Garcia, Megan C. Procario , Sanghee Yoo , Amber...

The NK cell checkpoint NKG2A maintains expansion capacity of human NK cells

Human natural killer (NK) cells are cytotoxic effector cells that are increasingly harnessed in cancer immunotherapy. NKG2A/CD94 is an inhibitory receptor on NK cells that has established regulatory functions in the direct interaction with target...

Authors: Kaulfuss M, Mietz J, Fabri A, Vom Berg J, Münz C, Chijioke O

SST Peer Reviewed

Human iPSC-derived liver co-culture spheroids to model liver fibrosis

The lack of adequate humanin vitromodels that recapitulate the cellular composition and response of the human liver to injury hampers the development of anti-fibrotic drugs. The goal of this study was to develop a human spheroid culture model to...

Authors: Laura Cools , Mina Kazemzadeh Dastjerd , Ayla Smout , Vincent Merens , Yuwei Yang , Hendrik...

Protocol for the design, conduct, and evaluation of prime editing in human pluripotent stem cells

Prime editing introduces single-nucleotide polymorphism changes, small dele tions, or insertions at a specific genome site without double-stranded DNA breaks or the need for the donor template. Here, we present a protocol to design, conduct, and...

Authors: Wu Y, Sidharta M, Zhong A, Persily B, Li M, Zhou T

SST Peer Reviewed

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Category

Research Area

Platform

Topic

Species

Characteristic

Format

Origin

Automated production of gene-modified chimeric antigen receptor T cells using the Cocoon Platform

Modulating mutational outcomes and improving precise gene editing at CRISPR-Cas9-induced breaks by chemical inhibition of end-joining pathways

Molecular and Physiological Effects of Browning Agents on White Adipocytes from Bone Marrow Mesenchymal Stromal Cells

Automation, Monitoring, and Standardization of Cell Product Manufacturing

Protocol to create phenotypic primary human hepatocyte cultures using the RASTRUM 3D cell model platform

A reference human induced pluripotent stem cell line for large-scale collaborative studies

A High-Throughput Microphysiological Liver Chip System to Model Drug-Induced Liver Injury Using Human Liver Organoids

The NK cell checkpoint NKG2A maintains expansion capacity of human NK cells

Human iPSC-derived liver co-culture spheroids to model liver fibrosis

Protocol for the design, conduct, and evaluation of prime editing in human pluripotent stem cells