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Are there any recommendations to avoid excessive mortality during T cell Nucleofection®?

Try to keep the DNA amount for Nucleofection® quite low (e.g. 1 µg plasmid DNA per 100 µl reaction) since higher DNA amounts might cause increasing toxicity and mortality. It is also important to use highly purified DNA, e.g. by using Endofree™...
Missing: stable lines

What is the tissue source of Lonza’s bronchial and tracheal epithelial cells?

Lonza’s Bronchial/Tracheal Epithelial Cells (both with and without Retinoic Acid; both normal and diseased) are isolated from the epithelial cells that line the airway of the bifurcation of the lungs and small sections of the bronchia and trachea...
Missing: stable

Can MycoZap™ Prophlyactic be used in cell cultures expressing a Tet repressor?

One of the MycoZap™ Prophylactic components interacts with the Tet repressor, leading to unwanted expression of genes expressed under the control of a Tet response element in lines. Therefore, MycoZap™ Prophylactic is not suited for cell cultures...
Missing: stable

Can heparin be excluded when culturing endothelial cells? What is the purpose of heparin in the endothelial cell culture media?

...be noted that the Bovine Brain Extract vial in both the EGM™ Media and the EGM™-MV Media contains a small amount of heparin. Please note that Lonza cannot guarantee cell performance if any of the recommended supplements are excluded....
Missing: stable lines

Are ciliated cells present in the SAEC’s?

There are usually ciliated cells present in the SAEC isolations, which shows that the tissue is good and healthy and that everything went well in the isolation process.  However, the cilia do not persist during submers culture...
Missing: stable lines

Can mycoplasma grow outside of a cell?

This is an area that is up for debate. There is evidence that mycoplasma can survive in the absence of cells, and some may proliferate, but as a rule they are much happier if cells are present.
Missing: stable lines

Can mycoplasma grow in the absence of cells?

There is evidence to suggest that mycoplasma can survive in the absence of cells, some may even proliferate, but as a rule they are much more viable if cells are present. 
Missing: stable lines

How are Lonza's mononuclear cells isolated?

Lonza's mononuclear cells are isolated from bone marrow, cord blood, or peripheral blood via density gradient centrifugation
Missing: stable lines

Where are Lonza's Bronchial Smooth Muscle Cells isolated from?

Lonza’s Bronchial Smooth Muscle Cells (both normal and diseased) are isolated from the bronchial tubes. The region used is around the bifurcation of the bronchial tubes to the lungs and small sections of the bronchia and trachea just off...
Missing: stable lines

I thawed and plated my Clonetics® embryonic rat or mouse neuronal cells and there are a lot of dead cells. What happened?

Cell death will usually be observed during the first few days of growth, resulting in cell debris in the culture – this is normal. Cell cultivation should be continued and surviving cells should start to develop. By day 4, neurite networks...
Missing: stable lines
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