Data Type

Category

+ Show All

Research Area

+ Show All
320 results sorted by

Do mesenchymal stem cells cultured in TheraPEAK® MSCGM™ Mesenchymal Stem Cell Growth Medium BulletKit® require an extracellular matrix/plate coating?

While, cells may be able to be cultured in TheraPEAK® Mesenchymal Stem Cell Growth Medium (TheraPeak® MSCGM™) without an extracellular matrix/plate coating, for peak performance, a 5ug/mL fibronectin coating should be used. Alternative plate...
Missing: stable lines

What kind of protocol you recommend for stimulation of human T cells?

For getting the results we present in our datasheet, we recommend the follwing strategy: Cells should be stimulated and cultivated for 3 days before Nucleofection, preferentially on plates coated with 1 µg/ml anti-CD3 and 2 µg/ml anti-CD28 antibody...
Missing: stable lines

Can heparin be excluded when culturing endothelial cells? What is the purpose of heparin in the endothelial cell culture media?

...be noted that the Bovine Brain Extract vial in both the EGM™ Media and the EGM™-MV Media contains a small amount of heparin. Please note that Lonza cannot guarantee cell performance if any of the recommended supplements are excluded....
Missing: stable lines

Are ciliated cells present in the SAEC’s?

There are usually ciliated cells present in the SAEC isolations, which shows that the tissue is good and healthy and that everything went well in the isolation process.  However, the cilia do not persist during submers culture...
Missing: stable lines

Can mycoplasma grow outside of a cell?

This is an area that is up for debate. There is evidence that mycoplasma can survive in the absence of cells, and some may proliferate, but as a rule they are much happier if cells are present.
Missing: stable lines

Can mycoplasma grow in the absence of cells?

There is evidence to suggest that mycoplasma can survive in the absence of cells, some may even proliferate, but as a rule they are much more viable if cells are present. 
Missing: stable lines

Are there any recommendations to avoid excessive mortality during T cell Nucleofection®?

Try to keep the DNA amount for Nucleofection® quite low (e.g. 1 µg plasmid DNA per 100 µl reaction) since higher DNA amounts might cause increasing toxicity and mortality. It is also important to use highly purified DNA, e.g. by using Endofree™...
Missing: stable lines

How are Lonza's mononuclear cells isolated?

Lonza's mononuclear cells are isolated from bone marrow, cord blood, or peripheral blood via density gradient centrifugation
Missing: stable lines

Where are Lonza's Bronchial Smooth Muscle Cells isolated from?

Lonza’s Bronchial Smooth Muscle Cells (both normal and diseased) are isolated from the bronchial tubes. The region used is around the bifurcation of the bronchial tubes to the lungs and small sections of the bronchia and trachea just off...
Missing: stable lines

I thawed and plated my Clonetics® embryonic rat or mouse neuronal cells and there are a lot of dead cells. What happened?

Cell death will usually be observed during the first few days of growth, resulting in cell debris in the culture – this is normal. Cell cultivation should be continued and surviving cells should start to develop. By day 4, neurite networks...
Missing: stable lines
PAGE 6

Privacy | Legal | About Lonza

© Lonza. All rights reserved.