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(67)
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(39)
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306 results sorted by
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newest first
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Where are Lonza’s Bronchial/Tracheal Epithelial
cell
s isolated from?
Lonza’s Bronchial/Tracheal Epithelial
Cells
(both with and without Retinoic Acid; both normal and diseased) are isolated from the epithelial
cells
that
line
the airway of the bifurcation of the lungs and small sections of the bronchia and trachea...
Missing:
stable
Are ciliated
cell
s present in the SAEC’s?
There are usually ciliated
cells
present in the SAEC isolations, which shows that the tissue is good and healthy and that everything went well in the isolation process. However, the cilia do not persist during submers culture...
Missing:
stable
lines
Can MycoZap™ Prophlyactic be used in
cell
cultures expressing a Tet repressor?
One of the MycoZap™ Prophylactic components interacts with the Tet repressor, leading to unwanted expression of genes expressed under the control of a Tet response element in
lines
. Therefore, MycoZap™ Prophylactic is not suited for
cell
cultures...
Missing:
stable
Can mycoplasma grow outside of a
cell
?
This is an area that is up for debate. There is evidence that mycoplasma can survive in the absence of
cells
, and some may proliferate, but as a rule they are much happier if
cells
are present.
Missing:
stable
lines
Can mycoplasma grow in the absence of
cell
s?
There is evidence to suggest that mycoplasma can survive in the absence of
cells
, some may even proliferate, but as a rule they are much more viable if
cells
are present.
Missing:
stable
lines
Can heparin be excluded when culturing endothelial
cell
s? What is the purpose of heparin in the endothelial
cell
culture media?
...be noted that the Bovine Brain Extract vial in both the EGM™ Media and the EGM™-MV Media contains a small amount of heparin. Please note that Lonza cannot guarantee
cell
performance if any of the recommended supplements are excluded....
Missing:
stable
lines
How are Lonza's mononuclear
cell
s isolated?
Lonza's mononuclear
cells
are isolated from bone marrow, cord blood, or peripheral blood via density gradient centrifugation
Missing:
stable
lines
Where are Lonza's Bronchial Smooth Muscle
Cell
s isolated from?
Lonza’s Bronchial Smooth Muscle
Cells
(both normal and diseased) are isolated from the bronchial tubes. The region used is around the bifurcation of the bronchial tubes to the lungs and small sections of the bronchia and trachea just off...
Missing:
stable
lines
Can calcium influx issues affect viability or differentiation in Stem
Cell
s after Nucleofection®?
When
cells
are transfected by Nucleofection®, transient pores are generated in the
cell
membrane. Generally, these pores disappear within 15 minutes of transfection, but if
cells
are plated immediately after Nucleofection® in medias...
Missing:
stable
lines
Is it possible to use frozen primary CD34+ hematopoietic stem
cell
s for Nucleofection®?
Yes. For cryopreserved PBMCs or enriched CD34 populations, we recommend culturing the thawed
cells
1-2 hours in culture medium before Nucleofection®. Any further enrichment procedure after thawing is not recommended. Viability of frozen nucleofected...
Missing:
stable
lines
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