Heparin is added because it creates a better environment for FGF binding. Removing heparin for one passage should not have a noticeable effect, however for successive passaging, effects on proliferation may become more significant. It should be noted that the Bovine Brain Extract vial in both the EGM™ Media and the EGM™-MV Media contains a small amount of heparin. Please note that Lonza cannot guarantee cell performance if any of the recommended supplements are excluded.