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Are there any contaminating glial cells in your Clonetics™ rat or mouse embryonic neuronal cells?

There will be a small amount of glial cells in the culture, these are not considered a contaminating cell type. The glial cells are necessary to help the neurons form their axons and dendrites, synchronize the activity of the axons, and remove...
Missing: stable lines

Is the age of the mice important for my mouse T cell Nucleofection®?

Yes. We recommend using mice between 6-12 weeks. Using mouse T cells isolated from younger or older animals for Nucleofection® may result in much lower transfection efficiencies and/or viabilities.
Missing: stable lines

Is the viability of cells from diseased or diabetic donors less than cells from normal donors?

We guarantee > 70 % viability for cells from both diseased/diabetic donors and normal donors.
Missing: stable lines

Where are Lonza’s Pulmonary Artery Smooth Muscle Cells (PASMC) cells isolated from?

...tissue source to properly isolate a sufficient number of cells. Lonza production estimates that 95% of the time, the pulmonary artery that is used for our PASMCs comes directly off the heart. It is rarely, if ever that we get the branched sections located...
Missing: stable lines

What kind of protocol you recommend for stimulation of human T cells?

For getting the results we present in our datasheet, we recommend the follwing strategy: Cells should be stimulated and cultivated for 3 days before Nucleofection, preferentially on plates coated with 1 µg/ml anti-CD3 and 2 µg/ml anti-CD28 antibody...
Missing: stable lines

What is the typical cell distribution for peripheral blood mononuclear cells (PBMC) catalog number CC-2702? 

We don't characterize each lot of these cells. Therefore, we don't make any guarantee regarding cell type distribution.Typically, you would expect to see approximately 60-80% T Cells, 10-20% monocytes, and the remaining cells would...
Missing: stable lines

What is the difference between Lonza’s ALI screened airway epithelial cells and the non-screened cells?

All of Lonza’s airway epithelial cells receive standard quality control testing in monolayer culture. After cells have passed Lonza’s initial monolayer quality control testing, a few lots receive additional quality control testing to monitor...
Missing: stable lines

I thawed and plated my ampoule of Clonetics™ Cells yesterday and most of the cells are floating. What happened?

Clonetics™ Cells will not be confluent on day one. After 24 hours, you will see few attached cells per field under the microscope. Aspirate off the media and the floating cells and replace with fresh, pre-warmed media. Those attached cells...
Missing: stable lines

Do mesenchymal stem cells cultured in TheraPEAK™ Mesenchymal Stem Cell Growth Medium (TheraPeak™ MSCGM™) require an extracellular matrix/plate coating?

While, cells may be able to be cultured in TheraPEAK™ Mesenchymal Stem Cell Growth Medium (TheraPeak™ MSCGM™) without an extracellular matrix/plate coating, for peak performance, a 5ug/mL fibronectin coating should be used. Alternative plate...
Missing: stable lines

Where are Lonza’s Bronchial/Tracheal Epithelial cells isolated from?

Lonza’s Bronchial/Tracheal Epithelial Cells (both with and without Retinoic Acid; both normal and diseased) are isolated from the epithelial cells that line the airway of the bifurcation of the lungs and small sections of the bronchia and trachea...
Missing: stable
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