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Do you have any information regarding differentiation of mouse ES cells after Nucleofection® with large plasmids?

It is unlikely that plasmid size would affect in vitro differentiation capability. Gene targeting constructs are often as large as 20 kb and if treated well the ES cells are perfectly capable of generating germ line chimaeras afterwards (indicating...
Missing: stable

Is there a special recommendation on Human T cell enrichment before Nucleofection®?

For Nucleofection® it is preferable to use Human T cell populations enriched by magnetic separation. For example, MACS™ Microbeads (Miltenyi) or by a rosetting method. For magnetic separation we recommend negative selection or detaching beads after...
Missing: stable lines

Is the viability of cells from diseased or diabetic donors less than cells from normal donors?

We guarantee > 70 % viability for cells from both diseased/diabetic donors and normal donors.
Missing: stable lines

Where are Lonza’s Pulmonary Artery Smooth Muscle Cells (PASMC) cells isolated from?

...tissue source to properly isolate a sufficient number of cells. Lonza production estimates that 95% of the time, the pulmonary artery that is used for our PASMCs comes directly off the heart. It is rarely, if ever that we get the branched sections located...
Missing: stable lines

Is the age of the mice important for my mouse T cell Nucleofection®?

Yes. We recommend using mice between 6-12 weeks. Using mouse T cells isolated from younger or older animals for Nucleofection® may result in much lower transfection efficiencies and/or viabilities.
Missing: stable lines

What is the typical cell distribution for peripheral blood mononuclear cells (PBMC) catalog number CC-2702? 

We don't characterize each lot of these cells. Therefore, we don't make any guarantee regarding cell type distribution.Typically, you would expect to see approximately 60-80% T Cells, 10-20% monocytes, and the remaining cells would...
Missing: stable lines

What is the difference between Lonza’s ALI screened airway epithelial cells and the non-screened cells?

All of Lonza’s airway epithelial cells receive standard quality control testing in monolayer culture. After cells have passed Lonza’s initial monolayer quality control testing, a few lots receive additional quality control testing to monitor...
Missing: stable lines

I thawed and plated my ampoule of Clonetics™ Cells yesterday and most of the cells are floating. What happened?

Clonetics™ Cells will not be confluent on day one. After 24 hours, you will see few attached cells per field under the microscope. Aspirate off the media and the floating cells and replace with fresh, pre-warmed media. Those attached cells...
Missing: stable lines

Where are Lonza’s Bronchial/Tracheal Epithelial cells isolated from?

Lonza’s Bronchial/Tracheal Epithelial Cells (both with and without Retinoic Acid; both normal and diseased) are isolated from the epithelial cells that line the airway of the bifurcation of the lungs and small sections of the bronchia and trachea...
Missing: stable

Can MycoZap™ Prophlyactic be used in cell cultures expressing a Tet repressor?

One of the MycoZap™ Prophylactic components interacts with the Tet repressor, leading to unwanted expression of genes expressed under the control of a Tet response element in lines. Therefore, MycoZap™ Prophylactic is not suited for cell cultures...
Missing: stable
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