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Do you have any information regarding differentiation of mouse ES cells after Nucleofection® with large plasmids?

It is unlikely that plasmid size would affect in vitro differentiation capability. Gene targeting constructs are often as large as 20 kb and if treated well the ES cells are perfectly capable of generating germ line chimaeras afterwards (indicating...
Missing: stable

Is the viability of cells from diseased or diabetic donors less than cells from normal donors?

We guarantee > 70 % viability for cells from both diseased/diabetic donors and normal donors.
Missing: stable lines

Is the age of the mice important for my mouse T cell Nucleofection®?

Yes. We recommend using mice between 6-12 weeks. Using mouse T cells isolated from younger or older animals for Nucleofection® may result in much lower transfection efficiencies and/or viabilities.
Missing: stable lines

Where are Lonza’s Pulmonary Artery Smooth Muscle Cells (PASMC) cells isolated from?

...tissue source to properly isolate a sufficient number of cells. Lonza production estimates that 95% of the time, the pulmonary artery that is used for our PASMCs comes directly off the heart. It is rarely, if ever that we get the branched sections located...
Missing: stable lines

What is the typical cell distribution for peripheral blood mononuclear cells (PBMC) catalog number CC-2702? 

We don't characterize each lot of these cells. Therefore, we don't make any guarantee regarding cell type distribution.Typically, you would expect to see approximately 60-80% T Cells, 10-20% monocytes, and the remaining cells would...
Missing: stable lines

What is the difference between Lonza’s ALI screened airway epithelial cells and the non-screened cells?

All of Lonza’s airway epithelial cells receive standard quality control testing in monolayer culture. After cells have passed Lonza’s initial monolayer quality control testing, a few lots receive additional quality control testing to monitor...
Missing: stable lines

I thawed and plated my ampoule of Clonetics™ Cells yesterday and most of the cells are floating. What happened?

Clonetics™ Cells will not be confluent on day one. After 24 hours, you will see few attached cells per field under the microscope. Aspirate off the media and the floating cells and replace with fresh, pre-warmed media. Those attached cells...
Missing: stable lines

What kind of protocol you recommend for stimulation of human T cells?

For getting the results we present in our datasheet, we recommend the follwing strategy: Cells should be stimulated and cultivated for 3 days before Nucleofection, preferentially on plates coated with 1 µg/ml anti-CD3 and 2 µg/ml anti-CD28 antibody...
Missing: stable lines

Do mesenchymal stem cells cultured in TheraPEAK™ Mesenchymal Stem Cell Growth Medium (TheraPeak™ MSCGM™) require an extracellular matrix/plate coating?

While, cells may be able to be cultured in TheraPEAK™ Mesenchymal Stem Cell Growth Medium (TheraPeak™ MSCGM™) without an extracellular matrix/plate coating, for peak performance, a 5ug/mL fibronectin coating should be used. Alternative plate...
Missing: stable lines

Are there any recommendations to avoid excessive mortality during T cell Nucleofection®?

Try to keep the DNA amount for Nucleofection® quite low (e.g. 1 µg plasmid DNA per 100 µl reaction) since higher DNA amounts might cause increasing toxicity and mortality. It is also important to use highly purified DNA, e.g. by using Endofree™...
Missing: stable lines
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