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Using the 96-well Shuttle® System : can I apply 96 different programs to a 96-well Nucleocuvette® Plate?

Yes, you can. Each well is pulsed individually. The plate setup is controlled via the 96-well Shuttle® Software on the laptop for the first generation 4D-Nucleofector® System
Missing: mycoalert

I thawed and plated my ampoule of Clonetics™ Cells yesterday and most of the cells are floating. What happened?

Clonetics™ Cells will not be confluent on day one. After 24 hours, you will see few attached cells per field under the microscope. Aspirate off the media and the floating cells and replace with fresh, pre-warmed media. Those attached cells will begin...
Missing: mycoalert

What nitrocellulose do you recommend for coating plates when culturing Lonza's rat cardiac myocytes?

Internally we use catalog number 162-0113 from Bio-Rad. 
Missing: mycoalert

Is it necessary to remove antibiotics for some time from the cell culture medium before taking sample to test medium for presence of mycoplasma using the MycoAlert® Mycoplasma Detection Kit?

The MycoAlert® Mycoplasma Detection Assay was designed to “fit” in with normal tissue culturing practices. It was developed in fully supplemented culture media containing antibiotics and DOES NOT require a period of culture without antibiotics...
Missing: plate

Using the 4D-Nucleofector® 96-well Unit: can I apply 96 different programs to a 96-well Nucleocuvette™ Plate?

Yes, you can. Each well is pulsed individually. The plate setup is controlled via the touchscreen of the 4D-Nucleofector® Core Unit.
Missing: mycoalert

Do mesenchymal stem cells cultured in TheraPEAK™ Mesenchymal Stem Cell Growth Medium (TheraPeak™ MSCGM™) require an extracellular matrix/plate coating?

While, cells may be able to be cultured in TheraPEAK™ Mesenchymal Stem Cell Growth Medium (TheraPeak™ MSCGM™) without an extracellular matrix/plate coating, for peak performance, a 5ug/mL fibronectin coating should be used. Alternative plate...
Missing: mycoalert

384-well Nucleofector® System: Can I apply 384 different programs to a 384-well Nucleocuvette® Plate?

Yes, you can. Each well is pulsed individually.
Missing: mycoalert

How much sample do you need for accurate results with MycoAlert® Assay? Do you need cells or just supernatant?

The standard protocol calls for taking 2 ml of culture media, spinning down any cells and removing 100 µl of supernatant as sample.Smaller initial aliquots of media may be taken to start with if necessary.
Missing: plate

I thawed and plated my Clonetics™ embryonic rat or mouse neuronal cells and there are a lot of dead cells. What happened?

Cell death will usually be observed during the first few days of growth, resulting in cell debris in the culture – this is normal. Cell cultivation should be continued and surviving cells should start to develop. By day 4, neurite networks will be...
Missing: mycoalert

384-well HT Nucleofector® System: Can I apply 384 different programs to a 384-well Nucleocuvette® Plate?

Yes, each well can be pulsed individually.
Missing: mycoalert
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