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A
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Lonza’s Smooth Muscle Cells are synthetic in their proliferating state. As the cells begin to differentiate, they become contractile.
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Hydrocortisone is used for at least two purposes in Lonza’s primary cell culture media. Hydrocortisone (in combination with low serum concentrations) will inhibit the takeover of the culture by fibroblasts which may be present in small numbers from...
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Yes, insulin can be left out of the SmGM™-2 media for as long as 1-3 passages.Insulin is beneficial to the cells for long term growth (ie. 4 - 5 passages), however smooth muscle cells do survive quite well short term without insulin.Insulin is not a...
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Heparin is added because it creates a better environment for FGF binding. Removing heparin for one passage should not have a noticeable effect, however for successive passaging, effects on proliferation may become more significant. It should be noted...
W
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Lonza’s Normal Human Osteoblasts (NHOst) are isolated by explant, and the tissue is from the spongy section of the bone head of the ribs, femur, or tibia.
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MSCs are prone to differentiate down an adipogenic lineage and some may accumulate lipids in the growth media or the differentiation kits other than the adipogenic differentiation media. MSCs are especially prone to spontaneous adipogenic...
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A
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A
s
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Optimal assay working temperature for all components is 18°C-22°C (for MycoAlert® Assay) and 20-22°C (for MycoAlert® PLUS Assay). In case room temperature significantly deviates from this optimal temperature range, it is recommended to use...
W
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L
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(
N
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A
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t
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For maximum re-differentiation and therefore maximum collagen production, Lonza’s Human Knee Articular Chondrocytes should be studied within the first passage after re-differentiation. Serial expansion in monolayer culture after re-differentiation...
W
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h
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m
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l
s
t
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m
c
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l
l
s
?
Lonza recommends the addition of TGF-B3 (PT-4124) to the Chondrogenic Differentiation Media (PT-3003) to promote chondrogenic differentiation of MSCs. Please see the MSC differenation protocol for complete details on differentiating MSCs down a...
H
o
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w
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d
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L
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a
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N
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m
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H
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m
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n
O
s
t
e
o
b
l
a
s
t
s
(
N
H
O
s
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Lonza’s Normal Human Osteoblasts (NHOst) generally appear fibroblastic. They are cuboidal in lower densities and become spindle shaped as they become confluent.
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