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What is the difference between each of the types of HUVECs that Lonza offers?

When choosing between the various types of HUVECs that Lonza offers, the choice can be made by answering the following questions:What media do you intend to culture the cells in?Do you require pooled or single donor cells?Do you require additional...

How do I handle my Lonza Control Standard Endotoxin (CSE) for Bacterial Endotoxin Testing (BET)?

Lyophilized E. coli endotoxin should be stored at 2-8°C. Endotoxin solutions are generally very stable upon storage except at more dilute concentrations. Rehydrated E. coli endotoxin should be stored refrigerated only (at 2-8°C and not frozen)....

What type of heparin is used with fresh bone marrow?

Lonza uses sodium heparin as the anticoagulant at a final concentration of 100 units/mL.

When performing the Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ recombinant Factor C (rFC), endotoxin detection kit how do I know my pipettes are working adequately?

Check your pipetting accuracy. This is important in every phase of LAL testing. Make sure you are adding the correct amounts of material. Good quality graduated pipettes are already calibrated by the manufacturer, but it is up to the user to use them...

Is Lonza's fresh bone marrow filtered?

The fresh bone marrow is filtered only if visible clots are present. If the technical assistance notices clots in the marrow, they pour the marrow over a filter which removes the clot from the aspirate. No other manipulation of the product takes...

What is a Clonetics™ or Poietics™ Basal Medium?

The Clonetics™ and Poietics™ Basal Media are primarily composed of trace metals, amino acids, vitamins, salts, and water. The basal medium contains no growth factors or antibiotics and is not sufficient to support cell attachment and growth. To the...

When performing the Lonza Bacterial Endotoxin Test (BET), LAL gel clot endotoxin detection kit how do I know if I have a positive result for gel clot?

A positive result is a firm gel which maintains its integrity through inversion to 180°. Resist the temptation to look at any of the test tubes before the hour is up. When reading your test results after the one-hour incubation gently withdraw each...

What is in your ReagentPack™ Subculturing Reagents Kit (CC-5034)?

The ReagentPack™ Subculturing Reagents Kit is composed of three components: - 100 ml of HEPES Buffered Saline Solution, - 100 ml of Trypsin/EDTA- 100 ml of Trypsin Neutralizing Solution (TNS).

Do I need to heat inactivate the FBS contained in the SingleQuots™ Kit?

No, we do not recommend heat inactivating the FBS provided in the SingleQuots™ Kit. Heat inactivating the serum can alter cell performance and denature essential components in the serum.

How should I prepare and handle my product samples for Bacterial Endotoxin Testing (BET) when using Lonza endotoxin detection assays? How do I adjust the pH?

As with handling of the lysate test reagents, the key word is cleanliness. Use aseptic and apyrogenic technique. Check the product for inhibition. Some products, especially members of the small volume parenteral family, may contain substances at...

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