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What is the difference between the EGM and EGM-2 growth media? What is the difference between the EGM-MV and EGM-2MV growth media?

The EBM™ and EBM™-2 basal media are essentially identical. The difference between the EGM™ and EGM™-2 growth media (or the EGM™-MV and EGM™-2MV growth media) are in the supplements.EGM™ and EGM™-MV growth media will contain the undefined reagent...

Where are Lonzas Small Airway Epithelial cells isolated from?

Lonza’s Small Airway Epithelial cells (both normal and diseased) are isolated from the distal portion of the lung in the 1 mm bronchiole area. These cells are isolated from airway tubules 1mm in diameter and smaller. Anything with a diameter greater...

What is the difference between the EGM and EGM-MV Growth Media? What is the difference between the EGM-2 and EGM-2MV Growth Media?

Lonza endothelial cell media ending with –MV (such as the EGM™-MV and EGM™-2MV Growth Media) are typically designated for use with microvascular endothelial cells (with some exceptions).Lonza endothelial cell media not ending with –MV (such as the...

What are the advantages of the Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene Recombinant Factor (rFC), endotoxin detection assays over the rabbit test?

Lonza LAL and PyroGene™ rFC Endotoxin detection assays are faster, have greater sensitivity, show less variability,, are less expensive, and are an alternative to animal model testing. These assays allow higher throughput and can also be automated on...

What is the difference between Lonzas ALI screened airway epithelial cells and the non-screened cells?

All of Lonza’s airway epithelial cells receive standard quality control testing in monolayer culture. After cells have passed Lonza’s initial monolayer quality control testing, a few lots receive additional quality control testing to monitor...

Which isotype of VEGF is used in the EGM®-2 and EGM®-2MV Growth Media?

The isotype of VEGF is used in the EGM®-2 and EGM®-2MV Growth Media is isotype 165.

What is the concentration of sugar in Lonzas endothelial cell media?

While the complete formulation of all of Lonza’s specialty media are proprietary, the EBM™, EBM™-2, and EBM™ phenol red free basal media will all contain D-glucose (dextrose) [CAS number 50-99-7] at exactly 1.0 g/L (5.55 mM). There are no other...

How should I properly heat my Lonza QCL-1000 Endpoint LAL Bacterial Endotoxin Test (BET) Assay?

Incubation is another area where assay performance can be affected. We recommend the use of a dry bath and heat block combination that is capable of maintaining 37 ±1 °C. The temperature should be monitored with a calibrated thermometer and checked...

Where is Lonza's fresh bone marrow collected from?

The bone marrow is collected from the iliac crest.

How should I handle the reconstituted Lonza LAL PYROGENT Gel Clot Bacterial Endotoxin Testing (BET) Reagent?

Rehydrated LAL reagent may be stored for as long as 24 hours, but it must be kept at 2-8 °C. Do not pre-incubate lysate in the LAL test tubes before adding samples to them. This is not necessary and may cause sensitivity loss in the LAL reagent. If...
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