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Do you have recommendations for the type of samples used for the MycoAlert® Mycoplasma Detection Kit and the MycoAlert® PLUS Mycoplasma Detection Kit? And do you have recommendations related to sample storage?

The MycoAlert® Mycoplasma Detection Kit and the MycoAlert® PLUS Mycoplasma Assay require the use of cell free supernatant only! Cells MUST be removed at 200xg for 5 mins.For optimal assay performance, supernatant should be tested...

Do antibiotics effect the MycoAlert® and MycoAlert® PLUS Mycoplasma Detection Kits?

Gentamycin and all commonly used antiboitics do not effect MycoAlert® and MycoAlert® PLUS Mycoplasma Detection Assay. We have tested amphotericin, ampicillin, cephalothin, kanamycin, lincomycin, in addition to gentamycin, penicillin and streptomycin...

What is the effect of pH on the Luciferase-based BioAssays (ViaLight Plus, MycoAlert®, ToxiLight®) Lonza is offering?

A low pH can certainly affect the luciferase system used in all of these products. The luciferase will not work if the pH is below 6.5 and performance will rapidly decay below pH 7.2. The reagents in these kits will buffer back to some degree but...

How can cells be cured of mycoplasma contamination?

Where contamination has occurred, and the sample absolutely cannot be discarded, the MycoZap™ Mycoplasma Elimination Reagent has been optimized to eliminate mycoplasma with minimal toxic effects on the cells.The MycoZap™ Reagent eliminates mycoplasma...

How much sample do you need for accurate results with MycoAlert® and MycoAlert® PLUS Mycoplasma Detection Kits? Do you need cells or just supernatant?

The standard protocol calls for taking 2 ml of culture media, spinning down any cells and removing 100 µl of supernatant. Smaller initial aliquots of media may be taken to start with if necessary.

What are differences between the endothelial cell types isolated from different locations in the body?

The group of Chi et al analayzed various endothelial cells. Tissue-specific expression patterns in different tissue microvascular endothelial cells suggest they are distinct differentiated cell types that play roles in the local physiology of...

What is the Cocoon® Technology? 

The Cocoon® Platform utilizes a single use, disposable cassette to functionally close and automate cell manufacturing. Primarily developed to automated all steps in CAR-T cell manufacturing, the Cocoon® Platform is being used for numerous...


Do you still support the Quasi Vivo® Culture System product line? 

For strategic reasons, Lonza has made the business decision to end our distribution agreement with Kirkstall Inc. for the Quasi Vivo® Culture System product line. As a result, all Quasi Vivo® System products are discontinued as of 29 September, 2019....

What is the definition of a chemically-defined medium?  

Chemically defined media have a known composition and contain only components of know structure and weight (no extracts or plant hydrolysates, no BSA, HSA) they does not contain any components of animal origin (ADCF). Chemically defined media...
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