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Will Lonza’s Normal Human Articular Chondrocytes (NHAC-kn) express collagen Type X? Are Lonza’s Normal Human Articular Chondrocytes (NHAC-kn) hypertrophic following re-differentiation?

Lonza guarantees that the Normal Human Articular Chondrocytes (NHAC-kn) will express collagen Type II and sulfated proteoglycans (can be stained with Alcian Blue) upon re-differentiation. Lonza’s recommended differentiation protocol does induce...

Are ciliated cells present in the SAEC’s?

There are usually ciliated cells present in the SAEC isolations, which shows that the tissue is good and healthy and that everything went well in the isolation process. However, the cilia do not persist during submers culture and are...

What are Silensomes™ HLM?

Silensomes™ HLM are pooled human liver microsomes (HLM) chemically and irreversibly inactivated for one specific cytochrome P450 (CYP450) activity (1A2, 2A6, 2B6, 2C8, 2C9, 2C19, 2D6, 2E1, 3A4) using a mechanism based inhibitor (MBI). Silensomes™ HLM...

What can Silensomes™ HLM be used for?

Silensomes™ HLM can be used in cytochrome P450 screening and phenotyping assays, to identify enzymes responsible for the metabolism of drug candidates. Silensomes™ HLM can be used to predict directly the in-vitro fm for a particular CYP450 enzyme (fm...

Where are Lonza's Bronchial Smooth Muscle Cells isolated from?

Lonza’s Bronchial Smooth Muscle Cells (both normal and diseased) are isolated from the bronchial tubes. The region used is around the bifurcation of the bronchial tubes to the lungs and small sections of the bronchia and trachea just off of the...

What is CYP450 phenotyping?

In vitro identification and measurement of the contribution of the major cytochrome P450 enzymes (CYP450s) involved in the metabolism of a new drug candidate is called “CYP450 phenotyping”. Information from these assays is used to predict the impact...

Is the classical Lucetta® Luminometer compatible with MycoAlert® PLUS Assay?

Yes. You can use the MycoAlert® mode of the classical Lucetta® Luminometer AAL-1001 for running MycoAlert® PLUS Assay. However, as the MycoAlert® mode was programmed based on standard MycoAlert® Assay and has not be adapted...

Where are Lonza's normal Adipose Derived Stem Cells isolated from?

Lonza's normal (non-diabetic) Adipose Derived Stem Cells (ADSCs) are isolated from lipoaspirate obtained from liposuction surgeries. As liposuction surgeries typically pool lipoaspirate from multiple locations, a single isolation source is not able...

Do you offer on-site maintencance services for the 4D-Nucleofector® Core, X, Y, 96well or LV Unit?

No, currently we can only offer in-house maintenance services, but we are working on a process for on-site services.

Could you extract cDNA from the lysed cells after detection using the ViaLight™ Plus BioAssay?

The lysis of the cells with the Cell Lysis Reagent included in the ViaLight™ Plus Kit should not affect the DNA. Whilst we haven't conducted this experiment ourselves we cannot see any real reason why it should not be possible to...

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