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515 results sorted by
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The protocol for culturing Lonza's Osteoclast Precursor (OCP) cells written for plating cells into a 96-well plate.
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To achieve optimal performance and convenience a microplate luminometer should be used. If reagent injectors are available on the instrument, these should be used since they enable full automation of the assay. If reagent injectors are...
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The LGM-3™ (catalog no. CC-3211) media contains gentamicin sulfate at a final concentration of 50 mg/L. LGM-3™ also does contain phenol red. Lonza does not currently offer a phenol red or antibiotic free version or alternative as a...
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The LGM-3™ Medium contains human recombinant insulin, human albumin, and human transferrin.
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Certain compounds will quench the light emission in bioluminescent reactions. Typically these are strong red dyes (however, the ViaLight ™ Plus Assay has been designed to function in all phenol red containing media commonly used in...
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Whole, unprocessed bone marrow typically contains 4 million to 10 million mononuclear cells per milliliter (mL).
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Lonza's CD34+ cells should be cultured according to Lonza's "Technical information and Instructions for Culturing Human CD34+ Cells"Upon thaw, we typically suggest that CD34+ cells be established in culture at a density of 20,000 to 200,000...
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No, cryopreserved cells should be stored in liquid or vapor phase nitrogen. Storing the cells at higher temperatures can irreversibly damage the cells.
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A population doubling is the total number of population doublings of a cell type since its initiation in vitro. The number of population doubling is calculated using the following equations:Cell count at day of passage /Cell count 24 hours after...
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The doubling time is the number of hours it takes for a cell population to double in number. Doubling time is calculated using the following equation:Number of hours from 24 hours after seeding until passage/Population Doublings = Doubling Time...
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