Q:

Are ciliated cells present in the SAEC’s?

A:

There are usually ciliated cells present in the SAEC isolations, which shows that the tissue is good and healthy and that everything went well in the isolation process.  However, the ciliated cells do not persist and are usually gone by the time the cells are cryopreserved in house.  You can stimulate ciliogenesis of these cells (by raising the culture to an air-liquid interface with different media).  The cells are induced to undergo differentiation to the ciliated cells.