There are usually ciliated cells present in the SAEC isolations, which shows that the tissue is good and healthy and that everything went well in the isolation process.  However, the cilia do not persist during submers culture and are usually gone by the time the cells are cryopreserved in house.  You can stimulate ciliogenesis of these cells by raising the culture to an air-liquid interface with differentiation media.

For re-differentiation of SAEC at Air-Liquid-Interface Culture we are offering the S-ALI Small Airway Air-Liquid Interface Medium BulletKit