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What cells make up the mononuclear cell population?

Mononuclear cells are any blood cells with a rounded nucleus including lymphocytes (T-cells, B-cells, and Natural Killer [NK] Cells), monocytes, and dendritic cells.
Missing: stable lines

Where are Lonza’s RPTEC cells CC-2553 isolated from? Is Lonza certain that these cells are truly RPTEC?

Lonza’s Renal Proximal Tubule Epithelial Cells (RPTEC) are isolated from the cortex of the kidney. A characteristic of kidney cortex epithelial isolation results in a population consisting of approximately 50%- 70% RPTEC. Lonza uses a proprietary...
Missing: stable lines

Can I use the pmaxGFP™ control plasmid with insect cells such as S2 cells or SF9 cells?

The pmaxGFP™ Vector provided in our Nucleofector® Kits is not expressed in insect cells. We strongly recommend an insect expression vector encoding a fluorescent protein or lacZ reporter as a positive control for your experiments [e.g. Novagen™’s...
Missing: stable lines

Your instructions for culturing of Clonetics™ Cells do not mention centrifuging the cells upon thawing. Won't the DMSO kill the cells?

We have seen on multiple occasions that the effects of centrifugation can be significantly more damaging than the effects of residual DMSO on primary cells. As such, unless specifically noted in the recommended culture protocol, instead...
Missing: stable lines

What is the percentage of CD34+ cells in the bone marrow mononuclear cell population?

CD34+ cells make up about 2% of the population in bone marrow mononuclear cells.
Missing: stable lines

With regards to mesenchymal stem cells, what are "Dexter cultures"?

Dexter culture refers to the method of isolating stem cells proposed by Dexter et al in their 1977 paper: Dexter TM, Allen TD, Lajtha LG. Conditions controlling the proliferation of haemopoietic stem cells in vitro. JCell Physiol 1977; 91: 335–344...
Missing: stable lines

Where are Lonza’s PrSMC cells isolated from?

Lonza’s Prostate Smooth Muscle Cells (PrSMC) are isolated from the stroma and the vasculature of the prostate. Unfortunately, there is no way to distinguish between smooth muscle cells from the stroma and smooth muscle cells from the vasculature...
Missing: stable lines

Where are Lonza’s PrEC cells isolated from?

Lonza obtains our Lonza’s Prostate Epithelial Cells (PrEC) from the glandular epithelium (parenchymal portion) of the prostate which spans the entire prostate and not from one specific region.
Missing: stable lines

Are there any contaminating glial cells in your Clonetics™ rat or mouse embryonic neuronal cells?

There will be a small amount of glial cells in the culture, these are not considered a contaminating cell type. The glial cells are necessary to help the neurons form their axons and dendrites, synchronize the activity of the axons, and remove...
Missing: stable lines

Is there a special recommendation on Human T cell enrichment before Nucleofection®?

For Nucleofectio® it is preferable to use Human T cell populations enriched by magnetic separation. For example, MACS™ Microbeads (Miltenyi) or by a rosetting method. For magnetic separation we recommend negative selection or detaching beads...
Missing: stable lines
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