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FAQ
(94)
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(21)
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(21)
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(20)
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(9)
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94 results sorted by
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What is the effect of pH on the Luciferase-based BioAssays (ViaLight™ Plus,
MycoAlert
®, ToxiLight®) Lonza is offering?
A low pH can certainly affect the luciferase system used in all of these products. The luciferase will not work if the pH is below 6.5 and performance will rapidly decay below pH 7.2. The reagents in these kits will buffer back to some degree but...
Missing:
plate
Do the Clonetics™ Rat and Mouse Astrocytes require an extracellular matrix for plating?
No, they simply need to be seeded onto tissue culture treated plastic. For best performance, we recommend to use positively charged plasticware
Missing:
mycoalert
Can
plate
dilutions schemes be transferred to WinKQCL™ Software if contained in a LIMS?
Yes, this information can be pulled from a LIMS if available. For example, an export created in CSV can be exported back into LIMS.
Missing:
mycoalert
What
plate
format is the protocol for culturing Lonza's Osteoclast Precursor (OCP) cells written for?
The protocol for culturing Lonza's Osteoclast Precursor (OCP) cells written for
plating
cells into a 96-well
plate
.
Missing:
mycoalert
What passage are your B-ALI™ (Bronchial Air Liquid Interface) cells when plating on transwells?
The cryopreserved epithelial cells, that are screened and guaranteed for differentiation in B-ALI™ (Bronchial Air Liquid Interface) are in P1, the cells
plated
in transwells are P2. We do not recommend expansion of the cells before...
Missing:
mycoalert
Which pipette tips are compatible with the 96-well Shuttle® Nucleocuvette®
Plate
s?
We recommend using special tips, e.g. epT.I.P.S™ (Eppendorf, Hamburg, Germany) or TallTips™ (Matrix Technologies, Hudson, NH, USA). You can contact our Scientific Support Team for a list of compatible tips, including tips for pipetting robots.
Missing:
mycoalert
Do I need to shake the
plate
after adding the Cell Lysis Reagent during the ViaLight™ Assay?
No, this is not recommended.Shaking the
plate
during this step can - cause frothing which will deflect the light signal away from the detection unit, - reducing the number of RLUs observed - and producing an artificially low result.
Missing:
mycoalert
PyroTec Pro: Can you run different samples (different treatment, dilution etc.) on one
plate
?
The system can accommodate a variety of samples on one
plate
with different dilutions applied to each sample. The system will apply whatever dilutions are assigned to each sample on the WinKQCL™ Software-generated endotoxin template. Each...
Missing:
mycoalert
Do you have any recommended methods for detaching human monocytes from the
plate
for assaying or before Nucleofection®?
We have had good results by incubating the cells in ice cold PBS for 10 minutes and then rinsing the
plates
. Alternatively, the cells can be detached without a medium change by gently pipetting the cell suspension up and down. Our experience has...
Missing:
mycoalert
Can I apply more than one program (transfection condition) per
plate
with the 96-well Shuttle® System?
With the 96-well Shuttle® system, it is possible to apply up to 96 different programs (transfection conditions) per 96-well Nucleocuvette®
Plate
. Therefore, optimization of Nucleofection® Conditions for a particular cell line can...
Missing:
mycoalert
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