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What plate format is the protocol for culturing Lonza's Osteoclast Precursor (OCP) cells written for?

The protocol for culturing Lonza's Osteoclast Precursor (OCP) cells written for plating cells into a 96-well plate.
Missing: mycoalert

Can I also use the Lucetta® 2 Luminometer for other assays besides the MycoAlert® Mycoplasma Detection Assay?

Yes. In addition to the tailor-made MycoAlert® Mode it also has a "Single Read" mode which is suited for unprocessed luminescence readings, e.g. ATP-based cell proliferation or cytotoxicity assays, or Luciferase reporter gene assays.
Missing: plate

What passage are your B-ALI™ (Bronchial Air Liquid Interface) cells when plating on transwells?

The cryopreserved epithelial cells, that are screened and guaranteed for differentiation in B-ALI™ (Bronchial Air Liquid Interface) are in P1, the cells plated in transwells are P2. We do not recommend expansion of the cells before...
Missing: mycoalert

What is the effect of pH on the Luciferase-based BioAssays (ViaLight™ Plus, MycoAlert®, ToxiLight®) Lonza is offering?

A low pH can certainly affect the luciferase system used in all of these products. The luciferase will not work if the pH is below 6.5 and performance will rapidly decay below pH 7.2. The reagents in these kits will buffer back to some degree but...
Missing: plate

Which pipette tips are compatible with the 96-well Shuttle® Nucleocuvette® Plates?

We recommend using special tips, e.g. epT.I.P.S™ (Eppendorf, Hamburg, Germany) or TallTips™ (Matrix Technologies, Hudson, NH, USA). You can contact our Scientific Support Team for a list of compatible tips, including tips for pipetting robots.
Missing: mycoalert

When performing the MycoAlert® Mycoplasma Detection Assay, why is it necessary to spin out the cells?

It is highly recommended that the samples are spun down prior to testing. If cells are present in the assay they will contribute to the initial reading producing a higher background. This in turn can decrease the difference between the background and...
Missing: plate

Do I need to shake the plate after adding the Cell Lysis Reagent during the ViaLight™ Assay?

No, this is not recommended.Shaking the plate during this step can - cause frothing which will deflect the light signal away from the detection unit, - reducing the number of RLUs observed - and producing an artificially low result.
Missing: mycoalert

PyroTec Pro: Can you run different samples (different treatment, dilution etc.) on one plate?

The system can accommodate a variety of samples on one plate with different dilutions applied to each sample. The system will apply whatever dilutions are assigned to each sample on the WinKQCL™ Software-generated endotoxin template. Each...
Missing: mycoalert

Do you have any recommended methods for detaching human monocytes from the plate for assaying or before Nucleofection®?

We have had good results by incubating the cells in ice cold PBS for 10 minutes and then rinsing the plates. Alternatively, the cells can be detached without a medium change by gently pipetting the cell suspension up and down. Our experience has...
Missing: mycoalert

Can I apply more than one program (transfection condition) per plate with the 96-well Shuttle® System?

With the 96-well Shuttle® system, it is possible to apply up to 96 different programs (transfection conditions) per 96-well Nucleocuvette® Plate. Therefore, optimization of Nucleofection® Conditions for a particular cell line can...
Missing: mycoalert
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