Search Results

What cell numbers can I transfect when using the 4D-Nucleofector® System?

The 4D-Nucleofector® System offers great flexibility in terms of cell numbers.Using the 4D-Nucleofector® X Unit it is possible to perform Nucleofection® in 100 µl and 20 µl Format.The 100 µl Nucleocuvette® Format is suited for high cell...

The basic protocol for adherent Nucleofection« of neurons using the 4D-Nucleofector® Y Unit recommends to plate 150,000 freshly isolated or 300,000 cryopreserved neurons. What can I do if do not have enough cells to perform several samples?

Once you have found your optimal conditions you can always try to reduce the recommended cell number. To find out if lower cell numbers have been successfully used already, please check our cell data base. If you would like to reduce the cell number...

How many samples can I transfect in parallel when using the 4D-Nucleofector® X Unit?

The 4D-Nucleofector® X Unit offers flexible throughput from 1 to 16 samples. When working with 100 µl single Nucleocuvettes® you can process two vessels in parallel. However subsequent handling of more than 2 vessels is eneased via the option to...

What microtiter plates would you recommend for running a MycoAlert® and MycoAlert® Plus Assay on a plate luminometer?

We would recommend either Porvair Sciences 204003 or Corning Costar 3912 plates to use with the MycoAlert® and MycoAlert® Plus Assay. Both of these are solid white 96-well plates and give low background and optimal results when used with the...

How many different primary cell kits are available for the 4D-Nucleofector® System?

In contrast to the Nucleofector® I, II and 2b System which comes with individual kits for each primary cell type, the 4D-Nucleofector® System only requires 5 primary cell kits covering a broad range of primary cells. There are cell-type...

I transfected neurons using the 4D-Nucleofector® Y-Unit. After Nucleofection®, there were precipitates in my culture. What can I do?

Most likely there was too much Nucleofector® Solution left before medium addition. Please try to aspirate solution after Nucleofection® as complete as possible without disturbing cell adherence, but add medium quickly to avoid cells getting...

What is the advantage of using conductive polymer as electrode material for the 4D-Nucleofector® X-Unit and 96-well Unit?

With conductive polymer electrodes the release of metal ions into the cell suspension is avoided. Conductive polymer electrode Nucloecuvette® Vessels are used in the 4D-Nucleofector® X-Unit and 96-well Unit?

What buffer conditions give best resolution for agarose electrophoresis?

For small DNA fragments (<1,000 bp) when recovery is not necessary, we recommend the use of 1X TBE Buffer. Gels made with TBE Buffer give sharper bands than gels made with TAE Buffer. TBE results in better resolution for closely spaced DNA bands....

Can I use the settings recommended for the Nucleofector® I/II or 2b System with my 4D-Nucleofector® System?

No, the 4D-Nucleofector® is optimized for conductive polymer electrodes, while the Nucleofector® I/II or 2b System is working with electrodes made of aluminium.We recommend doing a quick cell line optimization using our Cell Line...

How should I cast my agarose gels to get the best resolution?

We usually cast agarose gels 3 mm to 4 mm thick. The gel volume needed can easily be estimated by measuring the surface area of the casting chamber, then multiplying by the gel thickness. Thinner gels can be cast on GelBond™ Support Film, and/or cast...

Data Type

Category

Research Area