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Where are Lonza’s HSMM cells isolated from?

Lonza obtains our Human Skeletal Muscle Myoblasts( HSMM), both diseased and normal, from quadriceps or psoas muscle tissue.

Do you have a transfection protocol for cells from clouded leopard or fishing cat?

Yes, we have a excellent reference for this question. The clouded leopard (Neofelis nebulosa) is currently in vulnerable status according to the International Union for Conservation of Nature red list (2017). The Zoological Park Organziation of...

Where are Lonza’s Ventricular Normal Human Cardiac Fibroblast cells isolated from?

Lonza obtains our Ventricular Normal Human Cardiac Fibroblast (NHCF-V) from left ventricle.

Which program should I use for co-transfection of RNP and CRISPR/Cas9 complexes ? The paper "Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells from Aikiko Seki and Sascha Rutz showed different programs as you have in the Lonza optimized Nucleofector Protocols?

Their publication in the Journal of Experimental Medicine is an advanced optimization for gene knock out in primary human and mouse T cells. The final protocol achieved 85-98% gene knock out for several tested receptors.The group has tested and...

Which Nucleofector® Protocol should I use for genome editing and transfection of CRISPR/Cas9 into my cells?

Please use always the ready-to-use protocol for the specific cell type. The optimized programs are cell type specific and do work for any kind of substrates. You can transfect small and large DNA vectors, mRNA, siRNA, gRNA, peptides and proteins with...

What is the difference between a Tissue Acquisition Number (TAN) and a lot/batch number?

Each time Lonza receives a tissue sample, the entire tissue sample is assigned a unique Tissue Acquisition Number (TAN).However, each collection/production of final, prepared product will be assigned a unique lot/batch number.For many productions,...

Where are Lonza’s Atrial Normal Human Cardiac Fibroblast cells isolated from?

Lonza obtains our Atrial Normal Human Cardiac Fibroblast (NHCF-A) from right atrium.

Can I plate cells at a higher initial concentration than the Clonetics™ and Poietics™ plating protocol recommends? What is the minimum dilution of DMSO most cells can tolerate?

Lonza's recommended plating density for primary cells is established for two main reasons: To ensure residual DMSO is sufficiently diluted while cells are initially attaching and To allow a maximum number of population doublings per passage. With...

Do you have a Nucleofection® Protocol for bacterial transformation for the Nucleofection® I, IIN, IIS or 2b Systems?

Yes, we do have guidelines for bacteria transformation on the Nucleofector® Systems. However, not all models of the Nucleofector® System series are capable of bacteria transformation and have bacteria pulses implemented in the program list,...

Will Lonza’s HSMM cells de-differentiate after they have been differentiated to form myotubes?

The differentiation of Lonza’s Human Skeletal Muscle Myoblasts (HSMM) to myotubes is a terminal differentiation and, therefore, once differentiated, these cells will not de-differentiate to myoblasts. Additionally, differentiation is not...

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