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Where are Lonza’s PrSMC cells isolated from?

Lonza’s Prostate Smooth Muscle Cells (PrSMC) are isolated from the stroma and the vasculature of the prostate. Unfortunately, there is no way to distinguish between smooth muscle cells from the stroma and smooth muscle cells from the vasculature and...

Do you also sell the boxes or cases for the Nucleofector® Core, X, Y and LV Units?

Yes, see this list of part codes for the different types of transport boxes: Part code: AAK-1001Items: 4D-Nucleofector® Demo Suitcase (2 units)Suited for: Core + X or Core + Y or X + YPart...

Are Lonza’s melanocytes classified/categorized by the donor’s skin tone?

Lonza’s human melanocytes, both adult and neonatal, are currently only classified by the donor’s race. Lonza designates race as Caucasian (C), Black/African American (B), Asian (A), Hispanic (H), Other (O) (which can include Indian, Native American,...

Why are Lonza’s adult melanocytes cultured with Endothelin-3 (ET-3) while the neonatal melanocytes are not cultured with this reagent?

Adult melanocytes typically have a very slow doubling time, especially in comparison to neonatal melanocytes. Endothelin-3 (ET-3) is added to adult melanocytes only to improve the doubling time and, in the process, help to maintain the cells as...

Are Lonza’s human mammary epithelial cells tested for estrogen receptor response?

Unfortunately, Lonza has not thoroughly investigated the estrogen receptor response/response to estrogen of the mammary epithelial cells being isolated in the HMEC product.

What is the difference between a Tissue Acquisition Number (TAN) and a lot/batch number?

Each time Lonza receives a tissue sample, the entire tissue sample is assigned a unique Tissue Acquisition Number (TAN).However, each collection/production of final, prepared product will be assigned a unique lot/batch number.For many productions,...

Can I plate cells at a higher initial concentration than the Clonetics™ and Poietics™ plating protocol recommends? What is the minimum dilution of DMSO most cells can tolerate?

Lonza's recommended plating density for primary cells is established for two main reasons: To ensure residual DMSO is sufficiently diluted while cells are initially attaching and To allow a maximum number of population doublings per passage. With...

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