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Where are Lonza’s HRE Human Renal Epithelial cells CC-2556 isolated from?

Lonza’s Human Renal Epithelial (HRE) cells are an expansion of HNK (human neonatal kidney) cells. The kidneys are homogenized and the mixed population of cells are cultured in Lonza REGM™ Renal Epithelial Cell Growth Medium for one passage prior to...

Where are Lonza's human Mesenchymal Stem Cells isolated from?

Lonza's human Mesenchymal Stem Cells (hMSCs) PT-2501 are isolated from bone marrow withdrawn from bilateral punctures of the posterior iliac crests of normal volunteers between the ages of 18 and 45 years old. Please see the linked document for more...

Where are Lonza’s RPTEC cells CC-2553 isolated from? Is Lonza certain that these cells are truly RPTEC?

Lonza’s Renal Proximal Tubule Epithelial Cells (RPTEC) are isolated from the cortex of the kidney. A characteristic of kidney cortex epithelial isolation results in a population consisting of approximately 50%- 70% RPTEC. Lonza uses a proprietary...

Do you have a Nucleofection® Protocol for transfection of yeast (Saccharomyces cerevisiae)?

Unfortunately we do not have any records about Nucleofection® of yeast. It is also impossible to search for current publications, because you get only false positive hits. A lot of yeast genes have been nucleofected and many reportergene systems have...

With regards to mesenchymal stem cells, what are "Dexter cultures"?

Dexter culture refers to the method of isolating stem cells proposed by Dexter et al in their 1977 paper: Dexter TM, Allen TD, Lajtha LG. Conditions controlling the proliferation of haemopoietic stem cells in vitro. JCell Physiol 1977; 91: 335–344....

What is the general anatomy of the human prostate? From which portion of the human prostate are Lonza's prostate cells are derived?

The human prostate can generally be described as being glandular epithelium (parenchymal) surrounded by a fibromuscular stroma.The parenchymal portion of the prostate, or of any organ in the body, is epithelial like in nature and contains the “worker...

384-well Nucleofector® System: Can I apply 384 different programs to a 384-well Nucleocuvette® Plate?

Yes, you can. Each well is pulsed individually.

Can Lonza’s PrEC cells be co-cultured with Lonza’s PrSC cells?

While Lonza has never attempted to co-culture Prostate Epithelial Cells (PrEC) and Prostate Stromal Cells (PrSC) together in-house, the recommendation of Lonza Research & Development would be to plate and culture the PrEC and PrSC cells together...

Where are Lonza’s PrSC cells isolated from? What cells will be in the PrSC population?

Lonza’s Prostate Stromal Cells (PrSC) are isolated from the stroma of the prostate. Lonza’s Prostate Stromal Cells (PrSC) are a mixed population of cells, however, the majority of the cells in this population are fibroblastic in origin. It is not...

Where are Lonza’s PrEC cells isolated from?

Lonza obtains our Lonza’s Prostate Epithelial Cells (PrEC) from the glandular epithelium (parenchymal portion) of the prostate which spans the entire prostate and not from one specific region.
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