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I transfected neurons using the 4D-Nucleofector® Y unit. During analysis I observed areas with low or no cell density, even in my no pulse control sample.

Cells might have been disturbed or ran dry during pipetting steps. Please perform liquid removal and additions well-by-well. Avoid leaving neurons without any liquid coverage (medium or Nucleofector® Solution). To keep a small liquid film on the...

What is a BulletKit® Media?

A BulletKit® Media contains a bottle of basal medium and a SingleQuots® Kit. It is possible to purchase each of these components separately. Once the basal medium is supplemented with the SingleQuots® Kit, it becomes complete growth medium.

What are SingleQuots Kits?

A SingleQuots™ Supplements and Growth Factor Kit contains several vials that may include growth factors, FBS, and antibiotics in pre-measured aliquots - designed to be added to a bottle of basal medium. The concentrations of these factors are...

How often do mycoplasma contaminations occur in cell cultures?

A conservative estimate states that between 15-35% of all continuous cell cultures are contaminated with mycoplasma (Drexler HG, Uphof CC; 2002), some estimates are even higher (up to 80 % in some countries (Koshimizu K, Kotani H; 1981).

Do I need to add antibiotics to your Clonetics Media? What is GA-1000?

For most Clonetics™ Media, gentamicin-amphotericin-B mixture is provided at a final concentration of 30 µg/ml gentamicin and 15 ng/ml amphotericin-B. This antibiotic mixture is provided in a red-capped vial labeled GA-1000.

Can I use SDS as a detergent for a positive control if I do cytotoxicity assay with ToxiLight?

No. SDS has a negative influence on Adenylate Kinase (AK) activity. SDS is a cationic detergent, it is a very powerful protein denaturing agent, you will be aware of its use in SDS-PAGE for those reasons. ToxiLight™ works by measuring the activity of...

I would like to use the dipping electrode array for less than 24 wells. How can I keep non-required dipping electrodes clean?

We would recommend to prepare the culture plate as such that cells are only plated into those wells that should be transfected and leave further wells empty.After withdrawing the dipping electrode array from the plate you can wipe off residual liquid...

Which luminometer is suited for performing the MycoAlert® Assay?

For performing the MycoAlert® Assay in theory every luminometer is suitable as long as a specific sensitivity is given. Please find attached our list with luminometers we know to be suited to run the MycoAlert® and MycoAlert® Plus Mycoplasma...

Can I use a medium other than the one you offer to culture Clonetics® Cells?

We have an optimized medium for each Clonetics® Cell type. The cells are grown and tested in their specific medium system. Lonza guarantees the performance of Clonetics® cells only if appropriate Clonetics® media and reagents are used...

What kit sizes are available for the 4D-Nucleofector® X Unit?

We offer two different kit sizes for the 100 µl Nucleocuvette® Format (12 and 24 reactions) and one size for the 20 µl 16-well Nucleocuvette® Strip Format (32 reactions). 
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