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How old are the donors for Lonza’s dermal cells from neonatal donors?

The neonatal foreskin tissue used to isolate Lonza’s dermal cells from neonatal donors is almost always collected within the first few days after birth, and at most a week or two after birth. At most U.S. hospitals, circumcisions are performed before...

Why are Lonza’s adult melanocytes cultured with Endothelin-3 (ET-3) while the neonatal melanocytes are not cultured with this reagent?

Adult melanocytes typically have a very slow doubling time, especially in comparison to neonatal melanocytes. Endothelin-3 (ET-3) is added to adult melanocytes only to improve the doubling time and, in the process, help to maintain the cells as...

Are Lonza’s human mammary epithelial cells tested for estrogen receptor response?

Unfortunately, Lonza has not thoroughly investigated the estrogen receptor response/response to estrogen of the mammary epithelial cells being isolated in the HMEC product.

384-well Nucleofector® System: Can I apply 384 different programs to a 384-well Nucleocuvette® Plate?

Yes, you can. Each well is pulsed individually.

How do I promote keratinocyte terminal differentiation towards corneocytes?

Keratinocyte differentiation can be induced by increasing the concentration of calcium and/or by adding serum to the media. Cells should be initially plated using the standard culture media and conditions and allowed ample time to recover from...

How do I prevent keratinocyte terminal differentiation towards corneocytes? Can Lonza’s keratinocytes be cultured without calcium?

Keratinocyte differentiation can be induced by calcium in the culture media. As such, lowering the calcium concentration in a serum free media will greatly lower the chances keratinocytes terminally differentiating towards corneocytes. While Lonza...

Do the FlashGel™ Visualization Glasses protect against UV light?

Yes. They block UV exposure up to 400nm and “blue light” between 400 and 510nm. They meet OSHA requirements and ANSI Z87.1.

Where are Lonza’s NHOst CC-2538 cells isolated from?

Lonza obtains our Normal Human Osteoblasts (NHOst) from the spongy section of the bone head of ribs, femur, radius, fibula or tibia.If you would like to know, from which specific bone your lot of NHOst has been isolated, just contact our Scientific...

Where are Lonza’s NHAC-kn cells isolated from?

Lonza obtains our Normal Human Articular Chondrocytes (NHAC-kn) from the weight bearing cartilage of the knee (hyaline cartilage)

What nitrocellulose do you recommend for coating plates when culturing Lonza's rat cardiac myocytes?

Internally we use catalog number 162-0113 from Bio-Rad.

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