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How is the B-ALI™ Media System performance tested?

The media system is performance tested with our pre-screened NHBE cells and that system is guaranteed to form ciliated, mucous-producing cells after 3 weeks of culture.

Do Lonza's NK cells proliferate in culture?

Publications indicate that IL-2 and IL-21 can stimulate NK cell proliferation in addition to using a human serum supplemented medium.

How to maximize cell viability in a Nucleofection® experiment?

Pay careful attention to centrifugation speeds prior to Nucleofection®; be sure not to centrifuge the cells at higher than 90xg. Any trypsinization should use the minimal amount of reagent and time necessary in order to minimize stress to the cells,...

In Air-Liquid-Interface (ALI) Culture, won't the apical surface of B-ALI™ Cells dry out?

No, the cells secrete fluid at the apical side but also moisture is drawn through the cell layer from the basal layer.

What passage are your B-ALI™ (Bronchial Air Liquid Interface) cells when plating on transwells?

The cryopreserved epithelial cells, that are screened and guaranteed for differentiation in B-ALI™ (Bronchial Air Liquid Interface) are in P1, the cells plated in transwells are P2. We do not recommend expansion of the cells before...

Are Lonza's Natural Killer (NK) cells adherent in culture?

Resting Natural Killer cells grow in suspension, however, when IL-2 is added to the culture, they will adhere.

How many culture/trans-wells does your B-ALI™ Media Kit kit provide?

1 Ampoule of Normal Human Bronchial Epithelial Cells (NHBE) + 1 B-ALI™ Media Kit will make one 24-well plate for at least 1 month of culture & cells will display beating cilia within 25 days.

What growth factor is pertinent in chondrogenic differentiation of mesenchymal stem cells?

TGF-B3 has worked best in our hands so that is what we recommend.

Are there any substitutions that can be used in place of the HSA in the freezing media for hMSC's (Human Mesenchymal Stem Cells)?

Cryopreservation may compromise cell quality and performance. Lonza CANNOT guarantee performance of Clonetics™ & Poietics™ Cells that have been cryopreserved outside of Lonza. To avoid loss of cells and forfeiture of your warranty, we recommend...

Should any type of extracellular matrix be used when growing undifferentiated hMSC's (human Mesenchymal Stem Cells)?

That will depend on the type of medium used. If you are using our MSCGM™ BulletKit™ PT-3001, we do not recommend using a matrix with the cells as it might induce differentiation and make the cells difficult to detach from the culture vessel. If you...

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