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523 results sorted by
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Mononuclear cells are any blood cells with a rounded nucleus including lymphocytes (T-cells, B-cells, and Natural Killer [NK] Cells), monocytes, and dendritic cells.
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Lonza's CD14+ monocytes are isolated first by obtaining mononuclear cells from peripheral blood via density gradient centrifugation. The CD14+ cells are then isolated form the mononuclear cell population using either positive or negative...
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The recommended media for culturing Lonza's bone marrow stromal cells is MyeloCult™ H5100 from StemCell Technologies, Inc.
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The stromal layer is composed of many cell types including fibroblasts, mesenchymal stem cells, adipocytes, endothelial cells, and macrophages.
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Lonza's bone marrow stromal cells should be thawed according to Lonza's "Procedure for thawing Poietics™ Cells". Upon thaw, cells should be plated at 3,000-4,000 cells per cm² in MyeloCult™ H5100 from StemCell Technologies, Inc. An adherent...
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Internally we use catalog number 162-0113 from Bio-Rad.
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Yes, we have a excellent reference for this question. The clouded leopard (Neofelis nebulosa) is currently in vulnerable status according to the International Union for Conservation of Nature red list (2017). The Zoological Park Organziation of...
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Mesenchymal Cell Growth Supplement (MCGS) is 100% fetal bovine serum prescreened to neither induce nor prevent differentiation of human bone marrow derived stem cells PT-2501
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Their publication in the Journal of Experimental Medicine is an advanced optimization for gene knock out in primary human and mouse T cells. The final protocol achieved 85-98% gene knock out for several tested receptors.The group has tested and...
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Occasionally, though more frequently as outdoor temperatures begin to drop, media that Lonza recommends storing refrigerated will either completely freeze or partially freeze during shipping. While we do not suggest intentionally freezing and storing...
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