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What is the difference between the FBM Fibroblast Growth Basal Medium CC-3131 and the SCBM Stromal Cell Growth Basal Medium CC-3204? Can the FBM Basal Medium and the SCBM Basal Medium be used interchangeable? Is there a phenol red free version of the FBM Basal Medium?

The FBM™ Basal Medium and the SCBM™ Basal Medium are essentially the same except that the FBM™ Basal Medium contains phenol red and the SCBM™ Basal Medium does not. The FBM™ Basal Medium and the SCBM™ Basal Medium can always be used interchangeably...

Which Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene Recombinant Factor C (rFC), endotoxin detection kit should I use?

There are a few different things you should consider before deciding which endotoxin detection kit to order. First of all, how many samples will you be testing, and how frequently? If you are going to be testing one or two samples, one time only,...

What equipment do I need to run my Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene Recombinant Factor C (rFC), endotoxin detection assay?

Each endotoxin detection assay requires the use of different equipment. If running one of our PYROGENT™ Gel-Clot assays, a non-circulating waterbath or a dry heat block able to heat at 37°C is required. If running one of our kinetic assays,...

Can I use Nucleofection® Technology for transfecting Plasmodium falciparum?

Yes, but it depends on the platform. We would strongly recommend using the 4D-Nucleofector® or 96-well Shuttle™ System. The successful use of the 96-well Shuttle® System for transfection of P. falciparum has been published by Caro F, Miller...

Can I get a Material Data Safety Sheet (MSDS) for my Lonza endotoxin detection Bacterial Endotoxin Testing (BET) kit, LAL or PyroGene Recombinant Factor C (rFC), and/or my accessory products?

All of our endotoxin detection products have been evaluated and deemed non-hazardous in accordance with 29 CFR 1910.1200, the Hazard Communication Standard, based on the percentage quantities of its constituents. Therefore, none of our endotoxin...

When running a Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene Recombinant Factor C (rFC), endotoxin detection assay why should I use glass accesory products instead of plastic?

Endotoxin adheres to plastic surfaces more strongly than to glass surfaces. Therefore, we recommend that you use only borosilicate glass dilution tubes when preparing your Control Standard Endotoxin (CSE) dilutions. In some cases, certain plastics...

When running a Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene Recombinant Factor C (rFC), endotoxin detection kit why is it important to vortex my control standard endotoxin (CSE) dilutions?

Endotoxin will adhere to glass surfaces, but this can be counteracted with proper vortexing to ensure that the solution you aliquot into your reaction tubes or microplate has the proper EU/ml concentration. As our package inserts state, the CSE vial...

Is the Human Leukocyte Antigen (HLA) type information available for any of Lonza's cell products?

Lonza does obtain the Human Leukocyte Antigen (HLA) type information for many (but not all) of the Clonetics® and Poietics® Cell Types we are offering. If you require cells of a specific HLA type donor, please contact Lonza's Scientific Support Team....

Where are Lonzas aortic adventitial fibroblasts (AoAF) isolated from?

Lonza’s aortic adventitial fibroblasts (AoAF) are isolated from the outer connective tissue layer of the aorta. The aortia is divided into three layers. The tunica intima is the inner endothelial layer, the tunica media is the layers of the smooth...

How are Lonza's CD34+ cells isolated?

Lonza's CD34+ cells are isolated first by obtaining mononuclear cells from bone marrow or cord blood via density gradient centrifugation. The CD34+ cells are then isolated form the mononuclear cell population using positive immunomagnetic selection.
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