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What equipment do I need to run my Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor C (rFC), endotoxin detection assay?

Each endotoxin detection assay requires the use of different equipment. If running one of our PYROGENT™ Gel-Clot assays, a non-circulating waterbath or a dry heat block able to heat at 37°C is required. If running one of our kinetic assays,...

How many mononuclear cells are typically found per milliliter (mL) of whole, unprocessed bone marrow?

Whole, unprocessed bone marrow typically contains 4 million to 10 million mononuclear cells per milliliter (mL).

What are the suggested culturing conditions for CD34+ cells?

Lonza's CD34+ cells should be cultured according to Lonza's "Technical information and Instructions for Culturing Human CD34+ Cells"Upon thaw, we typically suggest that CD34+ cells be established in culture at a density of 20,000 to 200,000...

Is the Human Leukocyte Antigen (HLA) type information available for any of Lonza's cell products?

Lonza does obtain the Human Leukocyte Antigen (HLA) type information for many (but not all) of the Clonetics® and Poietics® Cell Types we are offering. If you require cells of a specific HLA type donor, please contact Lonza's Scientific Support Team....

Where are Lonza’s dermal cells isolated from?

Lonza is offering different dermal cell types, which are either isolated from adult or from neonatal tissue.Lonza’s adult dermal cells (including keratinocytes, microvascular endothelial cells, fibroblasts, and melanocytes) are usually isolated from...

How are Lonza's CD34+ cells isolated?

Lonza's CD34+ cells are isolated first by obtaining mononuclear cells from bone marrow or cord blood via density gradient centrifugation. The CD34+ cells are then isolated form the mononuclear cell population using positive immunomagnetic selection.

What is the purity of Lonza's CD34+ cells?

Cell purity (as determined via FACS analysis for CD34 expression) is guaranteed to be 90% or greater for all Lonza CD34+ cell products. For some Lonza CD34+ cell products, a "budget" version of the product is available. The "budget" versions of the...

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