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Why does Lonza suggest multiple concentrations of Hydrocortisone-21-hemisuccinate and ß-Glycerophosphate when differentiating Normal Human Osteoblasts (NHOst)?
...of hydrocortisone-21-hemisuccinate and/or ß-glycerophosphate. While high concentrations of these reagents may cause greater differentiation in some lots, the same concentrations may cause
cells
to slough and die in other lots. ???????As such, Lonza suggests trying varying...
Missing:
stable
lines
Is there any problem using the Vialight™ Plus Kit due to the presence of immobilized antibodies on the bottom of the microplate?
...antibody to be tested and detect the ATP by treating the wells as if they contained
cells
following the protocol contained in the kit. If you use the ViaLight HS kit add 100ul of NRR to each well and wait 5 min before adding 20ul of AMR. The difference...
Missing:
stable
lines
In my Nucleofection™ Experiment I see lower expression with my IRES construct in comparison with you pmax-GFP control. Do you have any information about the different expression profiles?
...the Nucleofector™ Technology" The data generated by our Research department show the different expression profiles of 3 vectors in HL-60 and HUVEC
cells
. You can see that our pmaxGFP cloned into three different vector sites show a different expression level....
Missing:
stable
lines
What is the difference between the EBM™ and EBM™-2 Basal Media? Can the EBM™-2 Basal Media be substituted with the EBM™ phenol red free Basal Media?
...The two basal media can be used interchangeably so long as the appropriate SingleQuot™ Kit supplements are added depending on the specific
cell
type being cultured. Additionally, both the EBM™ and EBM™-2 Basal Media can be substituted with the EBM™ Phenol...
Missing:
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lines
Are there positive controls for the MycoAlert® Assay?
...are designed for use with the MycoAlert® Mycoplasma Detection Kit and the MycoAlert® PLUS Mycoplasma Detection Kit for the detection of mycoplasma contaminations in
cell
cultures. The MycoAlert® Assay Control Set may be used to ensure that the operator and all...
Missing:
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I would like to use the dipping electrode array for less than 24 wells. How can I keep non-required dipping electrodes clean?
We would recommend to prepare the culture plate as such that
cells
are only plated into those wells that should be transfected and leave further wells empty.After withdrawing the dipping electrode array from the plate you can wipe off residual...
Missing:
stable
lines
What are the best time points for analysis of my siRNA experiments?
As the stability and half-life of various mRNAs and their protein products varies, it is important to empirically determine the best time points for assessing target knockdown. For example, it has been documented that in mammalian
cells
, mRNA...
Missing:
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What are the requirements for direct Nucleofection® of mRNA for protein expression?
The mRNA should be capped and polyadenylated. The conditions of Nucleofection® will be the same as for DNA with the particular
cell
type, i.e. follow the same protocol and use the same program, except one will likely need to add a much higher...
Missing:
stable
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Why am I not able to detect my fluorescent labeled siRNA (e.g. FITC) after 24 or 48 hours post-Nucleofection?
...analysis. Some labels are also subject to photo bleaching, pH changes, and may exhibit
cell
type specific effects as well. To minimize FITC-bleaching it may help to wrap the culture plates in aluminum foil immediately after Nucleofection™. So...
Missing:
stable
lines
Why can’t I obtain a Certificate of Analysis (COA) for a MycoAlert®/MycoAlert® PLUS/ToxiLight®/ViaLight® Kit via your Webpage-tool?
Lonza’s luminescent assays (including MycoAlert® Mycoplasma detection assay, MycoAlert® PLUS Mycoplasma detection assay, MycoAlert® Control set, ToxiLight® Non-Destructive Cytotoxicity Assay, and ViaLight® Plus
Cell
Proliferation...
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