Data Type
Cell Information
(515)
Citations
(4755)
FAQ
(306)
Culture Media
(45)
Category
Primary Cells and Media
(160)
Transfection
(106)
Bioassay
(31)
Mycoplasma Detection and Prevention
(16)
Laboratory Instrumentation
(14)
Serum-free and Speciality Media
(11)
Cell Lines and Primary Cancer Cells
(7)
3D Cell Culture
(5)
Classical Media and Reagents
(5)
Endotoxin Detection
(4)
Cell Services
(3)
Bioprocess Containers
(2)
Live Cell Imaging
(1)
+ Show All
Research Area
Basic Research
(100)
Cancer Research/Cell Biology
(67)
Immunotherapy / Hematology
(54)
Gene Expression
(39)
Stem Cells
(34)
Respiratory Research
(26)
Cardiovascular
(21)
Neurobiology
(18)
Regenerative medicine
(18)
Uncategorized
(18)
Toxicology
(15)
Dermatology/Tissue Engineering
(8)
Drug Discovery
(6)
Molecular Biology
(5)
Endotoxin Testing
(3)
Gastroenterology
(1)
Parasitology
(1)
+ Show All
306 results sorted by
relevance
alphabetical
newest first
oldest first
I would like to use the dipping electrode array for less than 24 wells. How can I keep non-required dipping electrodes clean?
We would recommend to prepare the culture plate as such that
cells
are only plated into those wells that should be transfected and leave further wells empty.After withdrawing the dipping electrode array from the plate you can wipe off residual...
Missing:
stable
lines
What are the best time points for analysis of my siRNA experiments?
As the stability and half-life of various mRNAs and their protein products varies, it is important to empirically determine the best time points for assessing target knockdown. For example, it has been documented that in mammalian
cells
, mRNA...
Missing:
stable
lines
What are the requirements for direct Nucleofection® of mRNA for protein expression?
The mRNA should be capped and polyadenylated. The conditions of Nucleofection® will be the same as for DNA with the particular
cell
type, i.e. follow the same protocol and use the same program, except one will likely need to add a much higher...
Missing:
stable
lines
Why am I not able to detect my fluorescent labeled siRNA (e.g. FITC) after 24 or 48 hours post-Nucleofection?
...analysis. Some labels are also subject to photo bleaching, pH changes, and may exhibit
cell
type specific effects as well. To minimize FITC-bleaching it may help to wrap the culture plates in aluminum foil immediately after Nucleofection™. So...
Missing:
stable
lines
Why can’t I obtain a Certificate of Analysis (COA) for a MycoAlert®/MycoAlert® PLUS/ToxiLight®/ViaLight® Kit via your Webpage-tool?
Lonza’s luminescent assays (including MycoAlert® Mycoplasma detection assay, MycoAlert® PLUS Mycoplasma detection assay, MycoAlert® Control set, ToxiLight® Non-Destructive Cytotoxicity Assay, and ViaLight® Plus
Cell
Proliferation...
Missing:
stable
lines
Do you have a protocol for the Nucleofection® of Plasmodium berghei using the Nucleofector® Systems
...strains with even better viability. They used program FF-167 in combination with P5 Primary
Cell
Solution. As the 96-well Shuttle™System also uses the conductive polymer electrodes, these conditions are transferable to the 4D-Nucleofector™ X Unit...
Missing:
stable
lines
PREVIOUS
PAGE
23
PAGE
24
PAGE
25
PAGE
26
PAGE
27
PAGE
28
PAGE
29
PAGE
30
PAGE
31
NEXT