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Which reporter gene should I choose for my reporter gene experiment?
In general, all reporter genes can be used.For some applications (e.g. reporter gene experiments in various primary
cells
, experiments with late analysis time point due to starving and /or stimulation) a reporter gene with a longer half life than...
Missing:
stable
lines
Where are Lonza’s aortic adventitial fibroblasts (AoAF) isolated from?
...muscle
cells
, and the tunica adventitia is the outermost layer where the aortic adventitial fibroblasts (AoAF) are isolated from. Aortic adventitial fibroblasts (AoAF) are isolated from either ascending or descending aorta....
Missing:
stable
lines
Why are Lonza’s adult melanocytes cultured with Endothelin-3 (ET-3) while the neonatal melanocytes are not cultured with this reagent?
Adult melanocytes typically have a very slow doubling time, especially in comparison to neonatal melanocytes. Endothelin-3 (ET-3) is added to adult melanocytes only to improve the doubling time and, in the process, help to maintain the
cells
...
Missing:
stable
lines
What does the message "weak" mean on the display of the Nucleofector® IIN, IIS or 2b after Nucleofection®?
...expected. So it is worthwhile to analyze the sample, keeping in mind that the transfection efficiency might be a bit lower. We don't recommend applying the pulse to the same cuvette again, as this will most likely result in high mortality of your
cells
....
Missing:
stable
lines
How to prepare a BulletKit™ Medium?
...media can be stored at 4°C for up to 30 days. Exceptions include the ALI differentiation media, mouse and rat neuronal media, and preadipocyte media. Please see the specific
cell
instructions for more information....
Missing:
stable
lines
What is the definition of a chemically-defined medium?
...can contain recombinant proteins. Due to current developments in the
cell
and gene therapy industry, there has been an increased focus on contaminants that may cause unfavorable reactions with patient material. ...
Missing:
stable
lines
How can I run a standard curve for ToxiLight™ Non-Destructive Cytotoxicity BioAssay?
...a sample of their untreated
cells
at 56°C for 1h.Measuring this and then comparing it with the RLUs produced from their samples will enable them to determine percent cytoxicity if that is what they require....
Missing:
stable
lines
What is the effect of pH on the Luciferase-based BioAssays (ViaLight™ Plus, MycoAlert®, ToxiLight®) Lonza is offering?
...but obviously if the pH is very low it will not be able to do this adequately.ViaLight™ Plus
Cell
Proliferation and Cytotoxicity BioAssay should be the least affected by this as it works by acidifying the sample and then has a very strong buffering system...
Missing:
stable
lines
Why does Lonza suggest multiple concentrations of Hydrocortisone-21-hemisuccinate and ß-Glycerophosphate when differentiating Normal Human Osteoblasts (NHOst)?
...of hydrocortisone-21-hemisuccinate and/or ß-glycerophosphate. While high concentrations of these reagents may cause greater differentiation in some lots, the same concentrations may cause
cells
to slough and die in other lots. As such, Lonza suggests trying varying concentrations...
Missing:
stable
lines
Is there any problem using the Vialight™ Plus Kit due to the presence of immobilized antibodies on the bottom of the microplate?
...antibody to be tested and detect the ATP by treating the wells as if they contained
cells
following the protocol contained in the kit. If you use the ViaLight HS kit add 100ul of NRR to each well and wait 5 min before adding 20ul of AMR. The difference...
Missing:
stable
lines
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