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Keratinocyte differentiation can be induced by increasing the concentration of calcium and/or by adding serum to the media. Cells should be initially plated using the standard culture media and conditions and allowed ample time to recover from...
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Lonza recommends the addition of TGF-B3 (PT-4124) to the Chondrogenic Differentiation Media (PT-3003) to promote chondrogenic differentiation of MSCs. Please see the MSC differenation protocol for complete details on differentiating MSCs down a...
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Keratinocyte differentiation can be induced by calcium in the culture media. As such, lowering the calcium concentration in a serum free media will greatly lower the chances keratinocytes terminally differentiating towards corneocytes. While Lonza...
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All three of the keratinocyte media that Lonza offers have similar basal media and supplements and all are serum free, however, slight variations and modifications have made the KGM-Gold® BulletKit® Lonza’s preferred keratinocyte...
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Occasionally, though more frequently as outdoor temperatures begin to drop, media that Lonza recommends storing refrigerated will either completely freeze or partially freeze during shipping. While we do not suggest intentionally freezing and storing...
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Optimal assay working temperature for all components is 18°C-22°C (for MycoAlert® Assay) and 20-22°C (for MycoAlert® PLUS Assay). In case room temperature significantly deviates from this optimal temperature range, it is recommended to use...
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Lonza’s luminescent assays (including MycoAlert® Mycoplasma detection assay, MycoAlert® PLUS Mycoplasma detection assay, MycoAlert® Control set, ToxiLight® Non-Destructive Cytotoxicity Assay, and ViaLight® Plus Cell Proliferation...
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Both assays use the same assay principle, but MycoAlert® PLUS Assay luminesces much brighter than the original MycoAlert® Assay. Because of the higher light output MycoAlert® PLUS Assay may be used on luminometers that were not...
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Lonza’s SingleQuot™ Kits contain various supplements and growth factors (such as fetal bovine serum, antibiotics, or hydrocortisone). While each supplement and growth factor will have its own catalog and lot number, each supplement and growth factor...
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So long as you have the product catalog number and batch/lot number, Lonza Certificates of Analysis (COA) can be downloaded from https://bioscience.lonza.com/lonza_bs/US/en/coa/search . Please enter the product catalog number and batch/lot number...
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