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Do antibiotics effect the MycoAlert® and MycoAlert® PLUS Mycoplasma Detection Kits?

Gentamycin and all commonly used antiboitics do not effect MycoAlert® and MycoAlert® PLUS Mycoplasma Detection Assay. We have tested amphotericin, ampicillin, cephalothin, kanamycin, lincomycin, in addition to gentamycin, penicillin and streptomycin...

What is the effect of pH on the Luciferase-based BioAssays (ViaLight™ Plus, MycoAlert®, ToxiLight®) Lonza is offering?

A low pH can certainly affect the luciferase system used in all of these products. The luciferase will not work if the pH is below 6.5 and performance will rapidly decay below pH 7.2. The reagents in these kits will buffer back to some degree but...

Do you have a protocol for stimulation of mouse B cells?

Yes, if you want to transfect mouse B cells, we recommend to stimulate the cells before. The information is also available in the mouse B cell Nucleofection Protocol. Our R&D has used following combination : RPMI1640 supplemented with...

How can cells be cured of mycoplasma contamination?

Where contamination has occurred, and the sample absolutely cannot be discarded, the MycoZap™ Mycoplasma Elimination Reagent has been optimized to eliminate mycoplasma with minimal toxic effects on the cells.The MycoZap™ Reagent eliminates mycoplasma...

How much sample do you need for accurate results with MycoAlert® and MycoAlert® PLUS Mycoplasma Detection Kits? Do you need cells or just supernatant?

The standard protocol calls for taking 2 ml of culture media, spinning down any cells and removing 100 µl of supernatant. Smaller initial aliquots of media may be taken to start with if necessary.

What media should be used for spheroid formation with hepatocytes?

Lonza's QC uses HCM (CC-3198) supplemented with 20% FBS and 25 mM Hepes. No plating media is necessary when seeding for spheroids.

What is the typical cell distribution for peripheral blood mononuclear cells (PBMC) catalog number CC-2702?

We don't characterize each lot of these cells. Therefore, we don't make any guarantee regarding cell type distribution.Typically, you would expect to see approximately 60-80% T Cells, 10-20% monocytes, and the remaining cells would be...

What are differences between the endothelial cell types isolated from different locations in the body?

The group of Chi et al analayzed various endothelial cells. Tissue-specific expression patterns in different tissue microvascular endothelial cells suggest they are distinct differentiated cell types that play roles in the local physiology of...

Which mobilization regime has been used for the CD34+ cells from mobilized peripheral blood (4Y-101)? Can I also have the cells from donors treated with another mobilization regime?

The mobilized peripheral blood used for 4Y-101 is derived from peripheral blood mobilized with Neupogen® 10 mcg/kg/day x 5 days. The cryopreserved cells can be isolated from day 5 and/or day 6 collections. It is possible to request...

Are the Rat Aortic Smooth Muscle Cells R-ASM-580 derived from single donor or does each lot contain a pool from several animals?

The R-ASM-580 per lot contain pooled cells from 4-5 male Sprague Dawley rats

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