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How should I prepare and handle my product samples for Bacterial Endotoxin Testing (BET) when using Lonza endotoxin detection assays? How do I adjust the pH?

As with handling of the lysate test reagents, the key word is cleanliness. Use aseptic and apyrogenic technique. Check the product for inhibition. Some products, especially members of the small volume parenteral family, may contain substances at...

Are Lonza Bacterial Endotoxin Detection (BET) reagents FDA approved?

All Lonza LAL based products, as well as the facility they are produced in, are FDA licensed.

Where are Lonza’s PrEC cells isolated from?

Lonza obtains our Lonza’s Prostate Epithelial Cells (PrEC) from the glandular epithelium (parenchymal portion) of the prostate which spans the entire prostate and not from one specific region.

Are the horseshoe crabs killed or injured during the harvest process?

Lonza takes great care to not harm the crabs during the harvesting of their blood. During harvesting blood is collected by puncturing the septum on the underside of the animal with a small needle. The animal will only give as much blood as it can...

Where are Lonza’s PrSMC cells isolated from?

Lonza’s Prostate Smooth Muscle Cells (PrSMC) are isolated from the stroma and the vasculature of the prostate. Unfortunately, there is no way to distinguish between smooth muscle cells from the stroma and smooth muscle cells from the vasculature and...

Does Lonza have products for oven validation depyrogenation studies?

Lonza offers Endotoxin Challenge Vials with part code 00192568 and High Potency Endotoxin with part code 00193763 for oven validation.

What percentage of most microvascular lots are lymphatic derived cells (LEC)? What percentage of most microvascular lots are blood vessel derived cells (BEC)?

Each lot of our Human Microvasculare Endothelial Cells - dermal (CC-2543 and CC-2516) and our Human Microvasculare Endothelial Cells - Lung (CC-2527) is tested for the percentage of LEC (double-positive for CD31+ and Podoplanin) and BEC (positive for...

When preforming Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ recombinant Factor C (rFC), endotoxin detection kit to establish the potency of Endotoxin Challenge Vials (ECV) can I use a syringe to reconstitute the ECV and other assay reagents?

We do not recommend that a needle and syringe be used to reconstitute ECV or other LAL reagents. The plungers of plastic syringes are generally coated with a lubricant and tend to interfere with the assay. We recommend the tops be removed and diluent...

Where are Lonza’s lung microvascular endothelial cells (HMVEC-L) isolated from?

Lonza’s lung microvascular endothelial cells (HMVEC-L) are isolated from the tissue directly beneath the outer lining of the lung. All vessels isolated are smaller than can be seen with the naked eye.

Are Lonza’s melanocytes classified/categorized by the donor’s skin tone?

Lonza’s human melanocytes, both adult and neonatal, are currently only classified by the donor’s race. Lonza designates race as Caucasian (C), Black/African American (B), Asian (A), Hispanic (H), Other (O) (which can include Indian, Native American,...

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