Data Type
Cell Information
(513)
Citations
(4775)
FAQ
(306)
Culture Media
(45)
Category
Primary Cells and Media
(160)
Transfection
(106)
Bioassay
(31)
Mycoplasma Detection and Prevention
(16)
Laboratory Instrumentation
(14)
Serum-free and Speciality Media
(11)
Cell Lines and Primary Cancer Cells
(7)
3D Cell Culture
(5)
Classical Media and Reagents
(5)
Endotoxin Detection
(4)
Cell Services
(3)
Bioprocess Containers
(2)
Live Cell Imaging
(1)
+ Show All
Research Area
Basic Research
(100)
Cancer Research/Cell Biology
(67)
Immunotherapy / Hematology
(54)
Gene Expression
(39)
Stem Cells
(34)
Respiratory Research
(26)
Cardiovascular
(21)
Neurobiology
(18)
Regenerative medicine
(18)
Uncategorized
(18)
Toxicology
(15)
Dermatology/Tissue Engineering
(8)
Drug Discovery
(6)
Molecular Biology
(5)
Endotoxin Testing
(3)
Gastroenterology
(1)
Parasitology
(1)
+ Show All
306 results sorted by
relevance
alphabetical
newest first
oldest first
What does the message "weak" mean on the display of the Nucleofector® IIN, IIS or 2b after Nucleofection®?
...expected. So it is worthwhile to analyze the sample, keeping in mind that the transfection efficiency might be a bit lower. We don't recommend applying the pulse to the same cuvette again, as this will most likely result in high mortality of your
cells
....
Missing:
stable
lines
How to prepare a BulletKit™ Medium?
...media can be stored at 4°C for up to 30 days. Exceptions include the ALI differentiation media, mouse and rat neuronal media, and preadipocyte media. Please see the specific
cell
instructions for more information....
Missing:
stable
lines
What is the definition of a chemically-defined medium?
...can contain recombinant proteins. Due to current developments in the
cell
and gene therapy industry, there has been an increased focus on contaminants that may cause unfavorable reactions with patient material. ...
Missing:
stable
lines
How can I run a standard curve for ToxiLight™ Non-Destructive Cytotoxicity BioAssay?
...a sample of their untreated
cells
at 56°C for 1h.Measuring this and then comparing it with the RLUs produced from their samples will enable them to determine percent cytoxicity if that is what they require....
Missing:
stable
lines
What is the effect of pH on the Luciferase-based BioAssays (ViaLight™ Plus, MycoAlert®, ToxiLight®) Lonza is offering?
...but obviously if the pH is very low it will not be able to do this adequately.ViaLight™ Plus
Cell
Proliferation and Cytotoxicity BioAssay should be the least affected by this as it works by acidifying the sample and then has a very strong buffering system...
Missing:
stable
lines
Why does Lonza suggest multiple concentrations of Hydrocortisone-21-hemisuccinate and ß-Glycerophosphate when differentiating Normal Human Osteoblasts (NHOst)?
...of hydrocortisone-21-hemisuccinate and/or ß-glycerophosphate. While high concentrations of these reagents may cause greater differentiation in some lots, the same concentrations may cause
cells
to slough and die in other lots. ???????As such, Lonza suggests trying varying...
Missing:
stable
lines
Is there any problem using the Vialight™ Plus Kit due to the presence of immobilized antibodies on the bottom of the microplate?
...antibody to be tested and detect the ATP by treating the wells as if they contained
cells
following the protocol contained in the kit. If you use the ViaLight HS kit add 100ul of NRR to each well and wait 5 min before adding 20ul of AMR. The difference...
Missing:
stable
lines
In my Nucleofection™ Experiment I see lower expression with my IRES construct in comparison with you pmax-GFP control. Do you have any information about the different expression profiles?
...the Nucleofector™ Technology" The data generated by our Research department show the different expression profiles of 3 vectors in HL-60 and HUVEC
cells
. You can see that our pmaxGFP cloned into three different vector sites show a different expression level....
Missing:
stable
lines
What is the difference between the EBM™ and EBM™-2 Basal Media? Can the EBM™-2 Basal Media be substituted with the EBM™ phenol red free Basal Media?
...The two basal media can be used interchangeably so long as the appropriate SingleQuot™ Kit supplements are added depending on the specific
cell
type being cultured. Additionally, both the EBM™ and EBM™-2 Basal Media can be substituted with the EBM™ Phenol...
Missing:
stable
lines
Are there positive controls for the MycoAlert® Assay?
...are designed for use with the MycoAlert® Mycoplasma Detection Kit and the MycoAlert® PLUS Mycoplasma Detection Kit for the detection of mycoplasma contaminations in
cell
cultures. The MycoAlert® Assay Control Set may be used to ensure that the operator and all...
Missing:
stable
lines
PREVIOUS
PAGE
23
PAGE
24
PAGE
25
PAGE
26
PAGE
27
PAGE
28
PAGE
29
PAGE
30
PAGE
31
NEXT