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Do your ProCHO™ Media contain hypoxanthine and thymidine?

All of our ProCHO™ Media, doe not contain Thymidine and Hypoxanthine. So if the customer has a CHO Clone which is dhfr-negativ, he will need to add Thymidine and Hypoxanthine or otherwise the cells will not grow. If the customer has a CHO clone...
Missing: stable linesGuideline

What is the best incubation time for transient gene expression in Nucleofection® Experiments?

The optimal incubation time ranges from 2 to 6 days depending on various factors like the stability of the protein in the medium, potential toxicity of the protein, cell density and stability of the plasmid in the nucleus. To check the timepoint...
Missing: stable linesGuideline

Can I use larger cuvettes for my Nucleofection® Reaction? Can I use the cuvettes more than once?

No. The electrical parameters provided by the Nucleofector® System are optimized for the cuvettes contained in the Nucleofector® Kits. The cuvettes are single-use only. Using the cuvettes more than once will result in higher cell death and lower...
Missing: stable linesGuideline

Do bacteria lead to false positive MycoAlert® Results?

...is characterized by rapid appearance of turbidity in cell culture media. One of the dangers of mycoplasma infection is that they do not produce turbidity, hence the need for a test such as the MycoAlert® Assay....
Missing: stable linesGuideline

What is a Clonetics™ or Poietics™ Basal Medium?

The Clonetics™ and Poietics™ Basal Media are primarily composed of trace metals, amino acids, vitamins, salts, and water. The basal medium contains no growth factors or antibiotics and is not sufficient to support cell attachment and growth...
Missing: stable linesGuideline

Which reporter gene should I choose for my reporter gene experiment?

In general, all reporter genes can be used.For some applications (e.g. reporter gene experiments in various primary cells, experiments with late analysis time point due to starving and /or stimulation) a reporter gene with a longer half life than...
Missing: stable linesGuideline

Why are Lonza’s adult melanocytes cultured with Endothelin-3 (ET-3) while the neonatal melanocytes are not cultured with this reagent?

Adult melanocytes typically have a very slow doubling time, especially in comparison to neonatal melanocytes. Endothelin-3 (ET-3) is added to adult melanocytes only to improve the doubling time and, in the process, help to maintain the cells...
Missing: stable linesGuideline

Where are Lonza’s aortic adventitial fibroblasts (AoAF) isolated from?

...muscle cells, and the tunica adventitia is the outermost layer where the aortic adventitial fibroblasts (AoAF) are isolated from. Aortic adventitial fibroblasts (AoAF) are isolated from either ascending or descending aorta....
Missing: stable linesGuideline

What does the message "weak" mean on the display of the Nucleofector® IIN, IIS or 2b after Nucleofection®?

...expected. So it is worthwhile to analyze the sample, keeping in mind that the transfection efficiency might be a bit lower. We don't recommend applying the pulse to the same cuvette again, as this will most likely result in high mortality of your cells....
Missing: stable linesGuideline

How to prepare a BulletKit™ Medium?

...media can be stored at 4°C for up to 30 days. Exceptions include the ALI differentiation media, mouse and rat neuronal media, and preadipocyte media. Please see the specific cell instructions for more information....
Missing: stable linesGuideline
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