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Can I still use Clonetics®/Poietics® Basal Media if it froze/partially froze during shipping?

Occasionally, though more frequently as outdoor temperatures begin to drop, media that Lonza recommends storing refrigerated will either completely freeze or partially freeze during shipping. While we do not suggest intentionally freezing and storing...

When preparing to run a Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor C (rFC), endotoxin detection kit how is the potency of CSE determined?

The RSE/CSE procedure used by Lonza to determine CSE potency is found on the Certificate of Analysis for each matched kit. In general, dilutions of endotoxin from multiple vials of CSE are tested unknowns compared to a series of standards produced...

Why can’t I obtain a Certificate of Analysis (COA) for a MycoAlert®/MycoAlert® PLUS/ToxiLight®/ViaLight® Kit via your Webpage-tool?

Lonza’s luminescent assays (including MycoAlert® Mycoplasma detection assay, MycoAlert® PLUS Mycoplasma detection assay, MycoAlert® Control set, ToxiLight® Non-Destructive Cytotoxicity Assay, and ViaLight® Plus Cell Proliferation...

When performing a Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor C (rFC), endotoxin detection assay how should I handle the LAL reagent?

Lonza LAL reagent should be handled gently. After reconstitution, the contents of the vial will easily go into solution by either gently inverting or swirling the vial. Vortexing or vigorously shaking the reconstituted LAL reagent will cause it to...

Why can’t I obtain a Certificate of Analysis (COA) for a component within a SingleQuot™ Kit?

Lonza’s SingleQuot™ Kits contain various supplements and growth factors (such as fetal bovine serum, antibiotics, or hydrocortisone). While each supplement and growth factor will have its own catalog and lot number, each supplement and growth factor...

When performing a Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor C (rFC), endotoxin detection assay how should I prepare my sample for testing?

There are a few ways in which your sample can be prepared before testing it. Most samples only need to be diluted before they are tested with one of our endotoxin detection kits. In order to determine how far out you should dilute your sample, you...

What are the size of CD34+ Cells?

When resting, they are about 8-10 microns.

Where can I obtain a Certificate of Analysis (COA) for a product I purchased through Lonza?

So long as you have the product catalog number and batch/lot number, Lonza Certificates of Analysis (COA) can be downloaded from https://bioscience.lonza.com/lonza_bs/US/en/coa/search . Please enter the product catalog number and batch/lot number...

When performing a Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor C (rFC), endotoxin detection assay what pH should my sample be? What should I adjust it with?

Our endotoxin detection assays are optimized to work with samples with a pH range of 6-8. If the pH of your sample falls outside of this range you can adjust it with a buffer, or HCl or NaOH. Make sure the buffer you are using is endotoxin free by...

When storing my Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor (rFC), endotoxin detection kit the temperature of my refrigerator/freezer has been out of range. Are my reagents ok to use?

The normal storage temperature for LAL products is 2-8°C. The improper storage of the product for a short period of time should not cause a problem with the performance of the kit as long as the components have not been reconstituted. Lyophilized...

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