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Are ciliated cells present in the SAEC’s?

There are usually ciliated cells present in the SAEC isolations, which shows that the tissue is good and healthy and that everything went well in the isolation process. However, the cilia do not persist during submers culture and are...

Do your ProCHO™ Media contain hypoxanthine and thymidine?

All of our ProCHO™ Media, doe not contain Thymidine and Hypoxanthine. So if the customer has a CHO Clone which is dhfr-negativ, he will need to add Thymidine and Hypoxanthine or otherwise the cells will not grow. If the customer has a CHO clone...

What are Silensomes™ HLM?

Silensomes™ HLM are pooled human liver microsomes (HLM) chemically and irreversibly inactivated for one specific cytochrome P450 (CYP450) activity (1A2, 2A6, 2B6, 2C8, 2C9, 2C19, 2D6, 2E1, 3A4) using a mechanism based inhibitor (MBI). Silensomes™ HLM...

Could you extract cDNA from the lysed cells after detection using the ViaLight™ Plus BioAssay?

The lysis of the cells with the Cell Lysis Reagent included in the ViaLight™ Plus Kit should not affect the DNA. Whilst we haven't conducted this experiment ourselves we cannot see any real reason why it should not be possible to...

What can Silensomes™ HLM be used for?

Silensomes™ HLM can be used in cytochrome P450 screening and phenotyping assays, to identify enzymes responsible for the metabolism of drug candidates. Silensomes™ HLM can be used to predict directly the in-vitro fm for a particular CYP450 enzyme (fm...

What is CYP450 phenotyping?

In vitro identification and measurement of the contribution of the major cytochrome P450 enzymes (CYP450s) involved in the metabolism of a new drug candidate is called “CYP450 phenotyping”. Information from these assays is used to predict the impact...

Is there any problem using the Vialight™ Plus Kit due to the presence of immobilized antibodies on the bottom of the microplate?

There should be no problem with the use of the ViaLight™ Plus Kit with immobilized antibodies. You can if you wish confirm this with your own experiment by conducting a control experiment as follows:Coat triplicate wells of a 96 well plate with each...

Can I take the ToxiLight™ Non-Destructive Cytotoxicity BioAssay Kit to look at the kinetics of cell death?

Yes, by taking a sample of the culture media (usually 20µl) into a fresh luminometer plate this can be assayed with the Adenylate Kinase Detection Reagent supplied in the Toxilight kit to look for increased levels of AK over the control. The rest of...

Can I use the ToxiLight™ Non-Destructive Cytotoxicity BioAssay Kit to assess the effect of transfection procedures on the health of my cells?

Yes. Just by taking a sample of the culture medium (usually 20µl) into a fresh luminometer plate and assaying it using the ToxiLight™ Non-Destructive Cytotoxicity BioAssay Kit you can see if increased levels of Adenylate...

Did you know that by combining ToxiLight™ Non-Destructive Cytotoxicity BioAssay with ViaLight™ Plus Cell Proliferation and Cytotoxicity BioAssay you can assess proliferation, cytotoxicity and even cytostasis?

Proliferation shows increased Vialight™ Plus RLUs (Relative Light Units) but NO increase in ToxiLight™ RLUs*Cytotoxicity shows decreased Vialight™ Plus RLUs and increased ToxiLight™ RLUs*Cytostasis (where the cells are alive but unable to...

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