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How should I handle the reconstituted Lonza LAL PYROGENT™ Gel Clot Bacterial Endotoxin Testing (BET) Reagent?

Rehydrated LAL reagent may be stored for as long as 24 hours, but it must be kept at 2-8 °C. Do not pre-incubate lysate in the LAL test tubes before adding samples to them. This is not necessary and may cause sensitivity loss in the LAL reagent. If...

When is the ideal time to study Lonza’s Human Knee Articular Chondrocytes (NHAC-kn) after re-differentiation?

For maximum re-differentiation and therefore maximum collagen production, Lonza’s Human Knee Articular Chondrocytes should be studied within the first passage after re-differentiation. Serial expansion in monolayer culture after re-differentiation...

How does Lonza perform the cell count on the fresh, unprocessed bone marrow? Does Lonza guarantee a minimum viability for the fresh, unprocessed bone marrow?

Lonza use a Coulter Counter to perform the total cell count on the fresh, unprocessed bone marrow. Lonza does not perform viability testing on this product, but when shipped overnight, viability is typically around 80%.

How do I handle my Lonza Control Standard Endotoxin (CSE) for Bacterial Endotoxin Testing (BET)?

Lyophilized E. coli endotoxin should be stored at 2-8°C. Endotoxin solutions are generally very stable upon storage except at more dilute concentrations. Rehydrated E. coli endotoxin should be stored refrigerated only (at 2-8°C and not frozen)....

How long does it take for the fresh bone marrow sample to arrive within the United states from the moment of harvest?

Barring any delays at airport customs, and assuming there are no weather related delays, the sample can be delivered anywhere in the continental United States within 26 hours of collection (same day delivery may be available for local deliveries,...

How would you describe the morphology of Lonza’s Normal Human Osteoblasts (NHOst)?

Lonza’s Normal Human Osteoblasts (NHOst) generally appear fibroblastic. They are cuboidal in lower densities and become spindle shaped as they become confluent.

When performing the Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ recombinant Factor C (rFC), endotoxin detection kit how do I know my pipettes are working adequately?

Check your pipetting accuracy. This is important in every phase of LAL testing. Make sure you are adding the correct amounts of material. Good quality graduated pipettes are already calibrated by the manufacturer, but it is up to the user to use them...

What is the percentage of purity of Lonza’s Normal Human Osteoblasts (NHOst) cells?

It is not easy to put an exact number to the purity of the NHOst populations because the tests used are all functional assays: alkaline phosphatase production and bone mineralization, which unless you have synchronized cultures, not all the cells are...

When performing the Lonza Bacterial Endotoxin Test (BET), LAL gel clot endotoxin detection kit how do I know if I have a positive result for gel clot?

A positive result is a firm gel which maintains its integrity through inversion to 180°. Resist the temptation to look at any of the test tubes before the hour is up. When reading your test results after the one-hour incubation gently withdraw each...

Are Lonza’s Normal Human Osteoblasts (NHOst) contact inhibited?

No, Lonza’s Normal Human Osteoblasts (NHOst) are not contact inhibited and tend to grow on top of each other after becoming confluent.

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