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523 results sorted by
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The bone marrow is collected from the iliac crest.
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Our endotoxin detection assays are optimized to work with samples with a pH range of 6-8. If the pH of your sample falls outside of this range you can adjust it with a buffer, or HCl or NaOH. Make sure the buffer you are using is endotoxin free by...
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When choosing between the various types of HUVECs that Lonza offers, the choice can be made by answering the following questions:What media do you intend to culture the cells in?Do you require pooled or single donor cells?Do you require additional...
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The normal storage temperature for LAL products is 2-8°C. The improper storage of the product for a short period of time should not cause a problem with the performance of the kit as long as the components have not been reconstituted. Lyophilized...
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Yes, insulin can be left out of the SmGM™-2 media for as long as 1-3 passages.Insulin is beneficial to the cells for long term growth (ie. 4 - 5 passages), however smooth muscle cells do survive quite well short term without insulin.Insulin is not a...
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Lonza uses sodium heparin as the anticoagulant at a final concentration of 100 units/mL.
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Lonza LAL and PyroGene™ rFC Endotoxin detection assays are faster, have greater sensitivity, show less variability,, are less expensive, and are an alternative to animal model testing. These assays allow higher throughput and can also be automated on...
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The fresh bone marrow is filtered only if visible clots are present. If the technical assistance notices clots in the marrow, they pour the marrow over a filter which removes the clot from the aspirate. No other manipulation of the product takes...
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Lonza’s Normal Human Osteoblasts (NHOst) are isolated by explant, and the tissue is from the spongy section of the bone head of the ribs, femur, or tibia.
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Incubation is another area where assay performance can be affected. We recommend the use of a dry bath and heat block combination that is capable of maintaining 37 ±1 °C. The temperature should be monitored with a calibrated thermometer and checked...
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