Search Results

What is the difference between each of the types of HUVECs that Lonza offers?

When choosing between the various types of HUVECs that Lonza offers, the choice can be made by answering the following questions:What media do you intend to culture the cells in?Do you require pooled or single donor cells?Do you require additional...

What is the difference between Lonza’s SAGM™ medium and the Growth Media portion of Lonza’s S-ALI BulletKit™ Medium?

Lonza’s SAGM™ medium and the Growth Media portion of Lonza’s S-ALI BulletKit™ Medium are essentially the same and can be used interchangeably (for example, cells could be cultured in Lonza’s SAGM™ medium just prior to differentiation using the...

What type of heparin is used with fresh bone marrow?

Lonza uses sodium heparin as the anticoagulant at a final concentration of 100 units/mL.

Is Lonza's fresh bone marrow filtered?

The fresh bone marrow is filtered only if visible clots are present. If the technical assistance notices clots in the marrow, they pour the marrow over a filter which removes the clot from the aspirate. No other manipulation of the product takes...

How does Lonza perform the cell count on the fresh, unprocessed bone marrow? Does Lonza guarantee a minimum viability for the fresh, unprocessed bone marrow?

Lonza use a Coulter Counter to perform the total cell count on the fresh, unprocessed bone marrow. Lonza does not perform viability testing on this product, but when shipped overnight, viability is typically around 80%.

What is the purpose of the 5-bromo-2'-deoxyuridine (BrdU) when culturing rat cardiac myocytes?

5-bromo-2'-deoxyuridine (BrdU) is very efficient at inhibiting the proliferation of fibroblasts which are natively present at the time of isolation. Without the addition of the BrdU, the fibroblasts would overtake the culture.

Can an alternate to the 5-bromo-2'-deoxyuridine (BrdU) be used to control the growth of fibroblasts when culturing rat cardiac myocytes?

At this time, we can only recommend the use of BrdU for controlling the growth of fibroblasts.When developing this product, we tried using cytosine beta-arabino furanoside (CAF) to inhibit fibroblast growth. While CAF successfully inhibited the...

What are some of the things I can customize on a precast agarose gel or molecular biology buffer?

Most aspects of a precast agarose gel can be customized, including: - well number, well spacing, well size/volume, - tier/row number, - gel size, - agarose type, agarose concentration, - stain, buffer, custom chemical additives, - packaging, labels,...

When performing a Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor C (rFC), endotoxin detection assay should I use matched reagents, lysate/CSE?

You need to use matched reagents in order to comply with FDA requirements for endotoxin testing. Each Lonza LAL lot is tested for functionality using United States Reference Standard EC-7. We then match this LAL lot to a lot of our Control...

How long does it take for the fresh bone marrow sample to arrive within the United states from the moment of harvest?

Barring any delays at airport customs, and assuming there are no weather related delays, the sample can be delivered anywhere in the continental United States within 26 hours of collection (same day delivery may be available for local deliveries,...

Data Type

Category

Research Area