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How is the B-ALI™ Media System performance tested?

The media system is performance tested with our pre-screened NHBE cells and that system is guaranteed to form ciliated, mucous-producing cells after 3 weeks of culture.
Missing: stable linesGuideline

What is the amount of DNA needed per well when using the 96-well Shuttle® Device?

The amount depends on the cell type and on the DNA construct. As a rule of thumb 400 ng of DNA per well should be applied. However, for some cells or constructs even 100 ng may be sufficient.
Missing: stable linesGuideline

What is the best way to centrifuge Human Hepatocytes prior to Nucleofection™?

In order to avoid cell compaction in the pellet and difficulties in cell resuspension, we recommend using round bottom 2 ml vials or 50 ml BD Falcon™ tubes for all centrifugation steps prior to Nucleofection™.
Missing: stable linesGuideline

What is the advantage of the Nucleofector® Technology over common electroporation (systems)?

High cell viability and high transfection efficiency with the Nucleofection® Technology are the most prominent features. Nucleofection®, i.e., the transfer of DNA into the nucleus and not only the cytosol, is essential when working...
Missing: stable linesGuideline

Can serum be used with Lonza's SAEC’s?

No. Serum will induce squamous differentiation of primary airway epithelial cells. Our SAGM™ Small Airway Epithelial Cell Growth Medium BulletKit CC-3118 does not contain FBS or any other serum.
Missing: stable linesGuideline

How many culture/trans-wells does your B-ALI™ Media Kit kit provide?

1 Ampoule of Normal Human Bronchial Epithelial Cells (NHBE) + 1 B-ALI™ Media Kit will make one 24-well plate for at least 1 month of culture & cells will display beating cilia within 25 days.
Missing: stable linesGuideline

I transfected neurons using the 4D-Nucleofector® Y-Unit. After Nucleofection®, there were precipitates in my culture. What can I do?

Most likely there was too much Nucleofector® Solution left before medium addition. Please try to aspirate solution after Nucleofection® as complete as possible without disturbing cell adherence, but add medium quickly to avoid cells...
Missing: stable linesGuideline

Can I use the settings recommended for the Nucleofector® I/II or 2b System with my 4D-Nucleofector® System?

No, the 4D-Nucleofector® is optimized for conductive polymer electrodes, while the Nucleofector® I/II or 2b System is working with electrodes made of aluminium.We recommend doing a quick cell line optimization using our Cell Line...
Missing: stable linesGuideline

What media can the CD14+ Monocytes be cultured in to maintain the monocyte phenotype?

CD14+ Monocytes can be cultured in IMDM + 10% FBS to help maintain the monocyte phenotype. The cells will slowly become adherent but it can take a week to get good adherence. M-CSF (10 ng/ml) will induce a little proliferation and increase adherence...
Missing: stable linesGuideline

Why does Lonza recommend two different concentrations of the B-Glycerophosphate in the Normal Human Osteoblasts (NHOst) differentiation protocol?

Lonza R&D has found some NHOst cell strains to be more sensitive to B-Glycerophosphate than others. While it is necessary to differentiate, at too high of concentrations the cells will lift off dish, curl up and die. As such, Lonza recommends...
Missing: stable linesGuideline
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