Data Type

Category

+ Show All

Research Area

+ Show All
313 results sorted by

Can I use the Nucleofector® Technology for RNAi applications? How do I start?

Yes. The Nucleofector® Technology can be easily applied for any RNAi substrate (siRNA, shRNA, miRNA). You can use the same conditions described in the cell type specific Optimized Protocol for DNA vectors (cDNA, shRNA or miRNA expressing plasmids...
Missing: stable linesGuideline

What is the Cocoon® Technology? 

The Cocoon® Platform utilizes a single use, disposable cassette to functionally close and automate cell manufacturing. Primarily developed to automated all steps in CAR-T cell manufacturing, the Cocoon® Platform is being used for numerous...
Missing: stable linesGuideline

Can customers buy PBMCs from COVID-19 infected donors? 

At this time, we cannot offer any cells from active COVID-19 infected donors. We are not completely ruling this out as a possibility for future custom offerings, but at this time we cannot say if/when we may be able to offer this. What we can offer...
Missing: stable linesGuideline

What are the difference between the KGM®, KGM®-2, KGM-Gold® BulletKit®?

...to be up to 20% faster and allows for additional population doublings when compared to cells cultured in the KGM™ Medium.In the second optimization of this medium, Lonza further modified the basal media and slightly modified the concentration of several...
Missing: stable linesGuideline

How is the B-ALI™ Media System performance tested?

The media system is performance tested with our pre-screened NHBE cells and that system is guaranteed to form ciliated, mucous-producing cells after 3 weeks of culture.
Missing: stable linesGuideline

What is the amount of DNA needed per well when using the 96-well Shuttle® Device?

The amount depends on the cell type and on the DNA construct. As a rule of thumb 400 ng of DNA per well should be applied. However, for some cells or constructs even 100 ng may be sufficient.
Missing: stable linesGuideline

What is the advantage of the Nucleofector® Technology over common electroporation (systems)?

High cell viability and high transfection efficiency with the Nucleofection® Technology are the most prominent features. Nucleofection®, i.e., the transfer of DNA into the nucleus and not only the cytosol, is essential when working...
Missing: stable linesGuideline

What is the best way to centrifuge Human Hepatocytes prior to Nucleofection™?

In order to avoid cell compaction in the pellet and difficulties in cell resuspension, we recommend using round bottom 2 ml vials or 50 ml BD Falcon™ tubes for all centrifugation steps prior to Nucleofection™.
Missing: stable linesGuideline

Can serum be used with Lonza's SAEC’s?

No. Serum will induce squamous differentiation of primary airway epithelial cells. Our SAGM™ Small Airway Epithelial Cell Growth Medium BulletKit CC-3118 does not contain FBS or any other serum.
Missing: stable linesGuideline

How many culture/trans-wells does your B-ALI™ Media Kit kit provide?

1 Ampoule of Normal Human Bronchial Epithelial Cells (NHBE) + 1 B-ALI™ Media Kit will make one 24-well plate for at least 1 month of culture & cells will display beating cilia within 25 days.
Missing: stable linesGuideline
PAGE 26

Privacy | Legal | About Lonza

© Lonza. All rights reserved.