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What cell number do I need per well when using the 96-well Shuttle® Device or the 4D-Nucleocuvette® Stripes?

The cell number is very much dependent on the cell type you use. For high throughput Nucleofection®, we usually start with one fifth of the optimal amount recommended for the standard Nucleofection® in the 100µl cuvette. Currently the absolute...
Missing: stable linesGuideline

In your optimized Nucleofection® Protocols for MDA-MB-231 and MDA-MB-468, you say to culture the cells without CO2. Why?

The media recommended for these cells is Leibovitz's L-15 medium which does not contain sodium bicarbonate to act as a buffer for the carbonic acid that would normally form in the presence of CO2. Furthermore, if the cells are cultured in Leibovitz...
Missing: stable linesGuideline

DNA-purity and Nucleofection®: Can low A260:A280 ratios lead to both reduced transfection efficiency and cell viability?

Yes. To check the quality of your DNA we strongly recommend determining the A260:A280 ratio. It should be ideally 1.8 - 2.0 for a good DNA preparation, but least 1.6.
Missing: stable linesGuideline

Can I use the ViaLight™ Plus Assay also, if my cells are cultured in 24well plates, 6well plates or 12 well plates?

To do the VialIght™ Plus Assay on larger volume tissue culture ( eg 6 well , 24 well) add the cell lysis reagent equal to half the volume in the plate ( ie 100 µl culture use 50 µl cell lysis). Wait 10 minutes for the lysis . Then follow...
Missing: stable linesGuideline

How does Lonza perform the cell count on the fresh, unprocessed bone marrow? Does Lonza guarantee a minimum viability for the fresh, unprocessed bone marrow?

Lonza use a Coulter Counter to perform the total cell count on the fresh, unprocessed bone marrow. Lonza does not perform viability testing on this product, but when shipped overnight, viability is typically around 80%.
Missing: stable linesGuideline

Are the Rat Aortic Smooth Muscle Cells R-ASM-580 derived from single donor or does each lot contain a pool from several animals?

The R-ASM-580 per lot contain pooled cells from 4-5 male Sprague Dawley rats
Missing: stable linesGuideline

I transfected neurons using the 4D-Nucleofector® Y unit. During analysis I observed areas with low or no cell density, even in my no pulse control sample.

Cells might have been disturbed or ran dry during pipetting steps. Please perform liquid removal and additions well-by-well. Avoid leaving neurons without any liquid coverage (medium or Nucleofector® Solution). To keep a small liquid film...
Missing: stable linesGuideline

How much sample do you need for accurate results with MycoAlert® and MycoAlert® PLUS Mycoplasma Detection Kits? Do you need cells or just supernatant?

The standard protocol calls for taking 2 ml of culture media, spinning down any cells and removing 100 µl of supernatant. Smaller initial aliquots of media may be taken to start with if necessary.
Missing: stable linesGuideline

Did you know that by combining ToxiLight™ Non-Destructive Cytotoxicity BioAssay with ViaLight™ Plus Cell Proliferation and Cytotoxicity BioAssay you can assess proliferation, cytotoxicity and even cytostasis?

Proliferation shows increased Vialight™ Plus RLUs (Relative Light Units) but NO increase in ToxiLight™ RLUs*Cytotoxicity shows decreased Vialight™ Plus RLUs and increased ToxiLight™ RLUs*Cytostasis (where the cells are alive but unable...
Missing: stable linesGuideline

When performing the PyroCell® Monocyte Activation Test, can I use a different cell culture medium than the IMDM (Iscove’s modified Dulbecco’s medium) recommended by Lonza?

Yes. However, the recommended medium has been validated with the PeliKine Human IL-6 ELISA Rapid Kit included in the PyroCell® MAT System. Please note that experience with alternative cell culture medium is limited. The IMDM 12-722F from Lonza...
Missing: stable linesGuideline
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