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Which Nucleofector® Protocol should I use for genome editing and transfection of CRISPR/Cas9 into my cells?

Please use always the ready-to-use protocol for the specific cell type. The optimized programs are cell type specific and do work for any kind of substrates. You can transfect small and large DNA vectors, mRNA, siRNA, gRNA, peptides and proteins...
Missing: stable linesGuideline

Do I need to use a gelatin, fibronectin, collagen, or Matrigel™ coating when culturing my Clonetics™ Cells?

Most Clonetics™ Cells do not require an extracellular matrix when grown on tissue culture treated plastic – flasks, dishes, well plates (with the exception of hepatocytes, and the rat/mouse neuronal and dorsal root ganglion neurons, rat cardiac...
Missing: stable linesGuideline

Does phenol red in the cell culture medium interfere with the ToxiLight™ Non-Destructive Cytotoxicity BioAssay?

The assay has been developed in the presence of phenol red so all the example data has been conducted in complete media. Phenol red does quench the light signal so if you compared assays in phenol red free media they would have higher RLUs. The...
Missing: stable linesGuideline

Does it matter if the PBS used for monocyte enrichment contains calcium and magnesium, if the cells should be used later in Nucleofection®?

The PBS should be calcium and magnesium free to prevent clumping of cells. We routinely use PBS without calcium and magnesium for example Lonza Cat. No. BEBP17-516Q
Missing: stable linesGuideline

What protocol should I use for Nucleofection® of patient derived blood samples, e.g. leukemia or lymphoma cells?

Unfortunately, we do not have a ready-to-use protocol for Chronic lymphocytic leukemia (CLL) or other blood cell derived cancer cells. The cells often have a chromosomal aberration and show the properties of a cell line. Therefore we suggest...
Missing: stable linesGuideline

Are the human mesenchymal stem cells (PT-2501) more prone to become differentiated down one lineage over the other?

...differentiation if allowed to reach confluence. As such, to maintain cells as undifferentiated cells, it is critical to subculture cells when they are subconfluent....
Missing: stable linesGuideline

Where are Lonza’s Normal Human Neural Progenitor (NHNP) and Normal Human Astrocytes (NHA) cells isolated from?

Lonza’s Normal Human Neural Progenitor (NHNP) and Normal Human Astrocytes (NHA) cells are primarily isolated from the cerebral cortex (or forebrain from cerebral hemisphere) from fragmented fetal brain tissue (16-20 weeks old).
Missing: stable linesGuideline

Why do you recommend 7AAD instead of PI staining for cell determination in mouse macrophages?

The results obtained using 7AAD staining are much clearer than those obtained using propidium iodide (PI).  Using flow cytrometry stained and unstained, transfected and non-transfected populations can be separated much more easily.
Missing: stable linesGuideline

Do I need to shake the plate after adding the Cell Lysis Reagent during the ViaLight™ Assay?

No, this is not recommended.Shaking the plate during this step can - cause frothing which will deflect the light signal away from the detection unit, - reducing the number of RLUs observed - and producing an artificially low result.
Missing: stable linesGuideline

Can I take the ToxiLight™ Non-Destructive Cytotoxicity BioAssay Kit to look at the kinetics of cell death?

Yes, by taking a sample of the culture media (usually 20µl) into a fresh luminometer plate this can be assayed with the Adenylate Kinase Detection Reagent supplied in the Toxilight kit to look for increased levels of AK over the control. The rest of...
Missing: stable linesGuideline
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