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320 results sorted by
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How many mononuclear
cell
s are typically found per milliliter (mL) of whole, unprocessed bone marrow?
Whole, unprocessed bone marrow typically contains 4 million to 10 million mononuclear
cells
per milliliter (mL).
Missing:
stable
lines
Can Lonza's Human Adipose-Derived Stem
Cell
s (hADSC) (PT-5006) be expanded prior to differentiation?
Lonza's human adipose derived stem
cells
(ADSC) are guarantee 5 passages prior to differentiation based on our existing data.
Missing:
stable
lines
Does phenol red in the
cell
culture medium interfere with the ToxiLight™ Non-Destructive Cytotoxicity BioAssay?
The assay has been developed in the presence of phenol red so all the example data has been conducted in complete media. Phenol red does quench the light signal so if you compared assays in phenol red free media they would have higher RLUs. The...
Missing:
stable
lines
What is the best practice for thawing pMAT
cell
s prior to use in the Pyro
Cell
® Monocyte Activation Test (MAT)?
The freeze medium for pMAT
cells
contain DMSO as a cryoprotectant that is toxic to thawed
cells
. To maintain full functionality of the pMAT
cells
, fast thawing and immediate dilution with complete medium is mandatory.Briefly, use a water...
Missing:
stable
lines
Can Lonza’s HSMM or SkMC
cell
s be maintained/subcultured/cryopreserved as myotubes after they have been differentiated?
The differentiation of Lonza’s Human Skeletal Muscle Myoblasts (HSMM) to myotubes is a terminal differentiation and, therefore, once differentiated, these
cells
will not de-differentiate to myoblasts. Differentiated HSMM myotubes can be maintained...
Missing:
stable
lines
Pyro
Cell
® MAT System: How do I store the
cell
culture medium before use in an MAT assay?
We recommend to store the unopened bottle of
cell
culture medium (IMDM) as stipulated by the manufacturer. An opened bottle shall be stored cooled (2-8°C).Before use with MAT assays, the
cell
culture medium should be equilibrated to room...
Missing:
stable
lines
Should any type of extra
cell
ular matrix be used when growing undifferentiated hMSC's (human Mesenchymal Stem
Cell
s)?
That will depend on the type of medium used. If you are using our MSCGM™ BulletKit™ PT-3001, we do not recommend using a matrix with the
cells
as it might induce differentiation and make the
cells
difficult to detach from the culture vessel. If you...
Missing:
stable
lines
In Air-Liquid-Interface (ALI) Culture, won't the apical surface of B-ALI™
Cell
s dry out?
No, the
cells
secrete fluid at the apical side but also moisture is drawn through the
cell
layer from the basal layer.
Missing:
stable
lines
How can I remove the
cell
s from the 100 uL Nucleocuvette® Vessel after Nucleofection®? Is there any alternative?
We recommend using the plastic single use pipettes provided in the kits. These pipettes prevent any damage to the
cells
and ensure a good
cell
recovery.
Missing:
stable
lines
Which Nucleofector® Protocol should I use for genome editing and transfection of CRISPR/Cas9 into my
cell
s?
Please use always the ready-to-use protocol for the specific
cell
type. The optimized programs are
cell
type specific and do work for any kind of substrates. You can transfect small and large DNA vectors, mRNA, siRNA, gRNA, peptides and proteins...
Missing:
stable
lines
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