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306 results sorted by
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Does phenol red in the
cell
culture medium interfere with the ToxiLight™ Non-Destructive Cytotoxicity BioAssay?
The assay has been developed in the presence of phenol red so all the example data has been conducted in complete media. Phenol red does quench the light signal so if you compared assays in phenol red free media they would have higher RLUs. The...
Missing:
stable
lines
Can Lonza’s HSMM or SkMC
cell
s be maintained/subcultured/cryopreserved as myotubes after they have been differentiated?
The differentiation of Lonza’s Human Skeletal Muscle Myoblasts (HSMM) to myotubes is a terminal differentiation and, therefore, once differentiated, these
cells
will not de-differentiate to myoblasts. Differentiated HSMM myotubes can be maintained...
Missing:
stable
lines
Should any type of extra
cell
ular matrix be used when growing undifferentiated hMSC's (human Mesenchymal Stem
Cell
s)?
That will depend on the type of medium used. If you are using our MSCGM™ BulletKit™ PT-3001, we do not recommend using a matrix with the
cells
as it might induce differentiation and make the
cells
difficult to detach from the culture vessel. If you...
Missing:
stable
lines
In Air-Liquid-Interface (ALI) Culture, won't the apical surface of B-ALI™
Cell
s dry out?
No, the
cells
secrete fluid at the apical side but also moisture is drawn through the
cell
layer from the basal layer.
Missing:
stable
lines
How can I remove the
cell
s from the 100 uL Nucleocuvette® Vessel after Nucleofection®? Is there any alternative?
We recommend using the plastic single use pipettes provided in the kits. These pipettes prevent any damage to the
cells
and ensure a good
cell
recovery.
Missing:
stable
lines
Which Nucleofector® Protocol should I use for genome editing and transfection of CRISPR/Cas9 into my
cell
s?
Please use always the ready-to-use protocol for the specific
cell
type. The optimized programs are
cell
type specific and do work for any kind of substrates. You can transfect small and large DNA vectors, mRNA, siRNA, gRNA, peptides and proteins...
Missing:
stable
lines
Do I need to use a gelatin, fibronectin, collagen, or Matrigel™ coating when culturing my Clonetics™
Cell
s?
Most Clonetics™
Cells
do not require an extracellular matrix when grown on tissue culture treated plastic – flasks, dishes, well plates (with the exception of hepatocytes, and the rat/mouse neuronal and dorsal root ganglion neurons, rat cardiac...
Missing:
stable
lines
Does it matter if the PBS used for monocyte enrichment contains calcium and magnesium, if the
cell
s should be used later in Nucleofection®?
The PBS should be calcium and magnesium free to prevent clumping of
cells
. We routinely use PBS without calcium and magnesium for example Lonza Cat. No. BEBP17-516Q
Missing:
stable
lines
Why do you recommend 7AAD instead of PI staining for
cell
determination in mouse macrophages?
The results obtained using 7AAD staining are much clearer than those obtained using propidium iodide (PI). Using flow cytrometry stained and unstained, transfected and non-transfected populations can be separated much more easily.
Missing:
stable
lines
Do I need to shake the plate after adding the
Cell
Lysis Reagent during the ViaLight™ Assay?
No, this is not recommended.Shaking the plate during this step can - cause frothing which will deflect the light signal away from the detection unit, - reducing the number of RLUs observed - and producing an artificially low result.
Missing:
stable
lines
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