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How many
cell
s do I have to use for each well of the 16-well Nucleocuvette® Strip?
This depends on the
cell
type you are working with. In our Optimized Protocols the
cell
numbers range from 2.5 x 10^4
cells
(mouse DC) up to 1x 10^6
cells
(human T
cells
). Detailed information is provided in our 4D-Nucleofector® Optimized Protocols.
Missing:
stable
lines
Are there any substitutions that can be used in place of the HSA in the freezing media for hMSC's (Human Mesenchymal Stem
Cell
s)?
Cryopreservation may compromise
cell
quality and performance. Lonza CANNOT guarantee performance of Clonetics™ & Poietics™
Cells
that have been cryopreserved outside of Lonza. To avoid loss of
cells
and forfeiture of your warranty, we recommend...
Missing:
stable
lines
When would be the optimal time to perform experiments post osteogenic differentiation for human mesenchymal stem
cell
s?
Once the
cells
have differentiated and started laying down bone matrix, we recommend assaying the
cells
immediately. If
cells
begin to delaminate (peel of the culture plate in layers), this would indicate that the
cells
are embedded in the calcium...
Missing:
stable
lines
Do you recommend a purification method to purify specific blood/immune
cell
populations before Nucleofection®?
We recommend using negative selection (depletion) because your purified
cells
are "untouched", not influenced, and strictly not activated.Positive selection may cause increasing amounts of dead
cells
and could also lead to activation of the
cells
...
Missing:
stable
lines
What passage are your B-ALI™ (Bronchial Air Liquid Interface)
cell
s when plating on transwells?
The cryopreserved epithelial
cells
, that are screened and guaranteed for differentiation in B-ALI™ (Bronchial Air Liquid Interface) are in P1, the
cells
plated in transwells are P2. We do not recommend expansion of the
cells
before...
Missing:
stable
lines
What type of matrix should your Clonetics™ embryonic rat/mouse neural
cell
s be seeded onto?
For optimal results, poly-D-lysine with laminin should be used, especially for the hippocampus
cells
.Poly-D-lysine without the laminin or poly-L-lysine could also be used for the rat and mouse neuronal
cells
.
Missing:
stable
lines
Could you extract cDNA from the lysed
cell
s after detection using the ViaLight™ Plus BioAssay?
The lysis of the
cells
with the
Cell
Lysis Reagent included in the ViaLight™ Plus Kit should not affect the DNA. Whilst we haven't conducted this experiment ourselves we cannot see any real reason why it should not be possible...
Missing:
stable
lines
How long will your Clonetics™ rat DRG's (dorsal root ganglion
cell
s) remain viable in culture?
The
cells
can remain viable in culture for about 28 days.
Missing:
stable
lines
How many mononuclear
cell
s are typically found per milliliter (mL) of whole, unprocessed bone marrow?
Whole, unprocessed bone marrow typically contains 4 million to 10 million mononuclear
cells
per milliliter (mL).
Missing:
stable
lines
Can Lonza's Human Adipose-Derived Stem
Cell
s (hADSC) (PT-5006) be expanded prior to differentiation?
Lonza's human adipose derived stem
cells
(ADSC) are guarantee 5 passages prior to differentiation based on our existing data.
Missing:
stable
lines
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