Data Type
Cell Information
(513)
Citations
(4772)
FAQ
(306)
Culture Media
(45)
Category
Primary Cells and Media
(160)
Transfection
(106)
Bioassay
(31)
Mycoplasma Detection and Prevention
(16)
Laboratory Instrumentation
(14)
Serum-free and Speciality Media
(11)
Cell Lines and Primary Cancer Cells
(7)
3D Cell Culture
(5)
Classical Media and Reagents
(5)
Endotoxin Detection
(4)
Cell Services
(3)
Bioprocess Containers
(2)
Live Cell Imaging
(1)
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Research Area
Basic Research
(100)
Cancer Research/Cell Biology
(67)
Immunotherapy / Hematology
(54)
Gene Expression
(39)
Stem Cells
(34)
Respiratory Research
(26)
Cardiovascular
(21)
Neurobiology
(18)
Regenerative medicine
(18)
Uncategorized
(18)
Toxicology
(15)
Dermatology/Tissue Engineering
(8)
Drug Discovery
(6)
Molecular Biology
(5)
Endotoxin Testing
(3)
Gastroenterology
(1)
Parasitology
(1)
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306 results sorted by
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When performing the MycoAlert® Mycoplasma Detection Assay, why is it necessary to spin out the
cell
s?
It is highly recommended that the samples are spun down prior to testing. If
cells
are present in the assay they will contribute to the initial reading producing a higher background. This in turn can decrease the difference between the background...
Missing:
stable
lines
Where are Lonza’s Human Aortic Smooth Muscle
Cell
s (AoSMC) isolated from?
While Lonza’s Human Aortic Smooth Muscle
Cells
(AoSMC) are isolated from either the ascending or descending aorta, it is usually the ascending aorta that is used. If the heart comes with the aorta, which happens most of the time, then the AoSMC...
Missing:
stable
lines
What testing methods are currently used to validate differentiation of Lonza's mesenchymal stem
cell
s?
Lonza currently validates trilineage differentiation of human mesenchymal stem
cells
using the following quantitative assays:Adipogenic Differentiation: lipid vacuole binding with AdipoRed (catalog no. PT-7009)Osteogenic Differentiation: inorganic...
Missing:
stable
lines
Are there any publications comparing Lonza's hepatocytes to hepatocytes derived from pluripotent stem
cell
s?
There is a citation from King's College London comparing Lonza's primary hepatocytes to stem-
cell
derived hepatocytes. Here is the link: https://sciencematters.io/articles/202004000002 Here are few key points from the article: CYP1A2...
Missing:
stable
lines
What is the age of the rats and mice the Clonetics™ embryonic neuronal
cell
s are isolated from?
Both are of embryonic origin: the rats are E18 or E19 and the mice are E14 or E15.
Missing:
stable
lines
What are Lonza's human Mesenchymal Stem
Cell
s (PT-2501) cryopreserved in?
The cryopreservation medium is: 85% MSCBM 10% DMSO 5% Human Serum Albumin (w/v)
Missing:
stable
lines
Where are Lonza’s Normal Human Osteoblasts (NHOst)
cell
s isolated from?
Lonza’s Normal Human Osteoblasts (NHOst) are isolated by explant, and the tissue is from the spongy section of the bone head of the ribs, femur, or tibia.
Missing:
stable
lines
I want to stimulate human primary T-
cell
s with anti-CD3 and anti-CD28. Do you have a detailed protocol for this?
Yes, the detailed protocol is part of our 4D-Nucleofector® Protocol for stimulated Human T
Cells
.For optimal stimulation of the transfected
cells
we recommend coating 96-wells plates for stimulation [Nunc Immuno™ Plate C96 Maxi Sorp™, Cat. No. 430...
Missing:
stable
lines
Can I use a medium other than the one you offer to culture Clonetics®
Cell
s?
We have an optimized medium for each Clonetics®
Cell
type. The
cells
are grown and tested in their specific medium system. Lonza guarantees the performance of Clonetics®
cells
only if appropriate Clonetics® media and reagents are used...
Missing:
stable
lines
What
cell
numbers can I transfect when using the 4D-Nucleofector® System?
The 4D-Nucleofector® System offers great flexibility in terms of
cell
numbers.Using the 4D-Nucleofector® X Unit it is possible to perform Nucleofection® in 100 µl and 20 µl Format.The 100 µl Nucleocuvette® Format is suited for high
cell
...
Missing:
stable
lines
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