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What concentration of Trypsin/EDTA do you recommend for subculturing primary cells?

For most cell types, Lonza recommends the Trypsin/EDTA provided as one of the components in our ReagentPack™ Subculturing Reagents Kit (CC-5034).The concentration of this solution is proprietary.
Missing: stable linesGuideline

What medium and which cytokines are recommended for Natural Killer (NK) cell culture?

For the cultivation of Natural Killer (NK) cells we recommend using the X-VIVO 15 medium.In regard to cytokines, this varies depending upon the application, but many cited publications indicate using IL-2 for activation and longer term culture.
Missing: stable linesGuideline

How many mycoplasma species exist? Which are the usual suspects in cell culture?

More than 180 species have been identified of which 20 distinct Mycoplasma and Acholeplasma species from human, bovine and swine have been isolated from cell culture. There are 6 species that account for 95% of all mycoplasma infections (M.orale...
Missing: stable linesGuideline

What is the shelf-life for all the media associated with Human mesenchymal stem cells?

The shelf-life of the MSCGM™ Mesenchymal Stem Cell Growth Medium BulletKit™ (PT-3001) once the SingleQuots Kit is added is 30 days. For the differentiation kits:hMSC differentiation BulletKit™ – osteogenic (PT-3002)hMSC differentiation...
Missing: stable linesGuideline

What additional donor information is available for Lonza’s diseased airway cells?

In addition to the standardly available donor information (age, gender, race), Lonza can provide the following additional information for most lots of our diseased airway cells: - smoking history (including volume and duration), - any medications...
Missing: stable linesGuideline

How old are the donors for Lonza’s dermal cells from neonatal donors?

The neonatal foreskin tissue used to isolate Lonza’s dermal cells from neonatal donors is almost always collected within the first few days after birth, and at most a week or two after birth. At most U.S. hospitals, circumcisions are performed...
Missing: stable linesGuideline

Does the basal media portion of the TheraPEAK™ Mesenchymal Stem Cell Growth Medium (TheraPeak™ MSCGM™) contain proteins?

The basal media portion of the TheraPEAK™ Mesenchymal Stem Cell Growth Medium (MSCGM™), catalog no BEBP12-934Q, contains recombinant and human serum derived proteins.
Missing: stable linesGuideline

How many different primary cell kits are available for the 4D-Nucleofector® System?

In contrast to the Nucleofector® I, II and 2b System which comes with individual kits for each primary cell type, the 4D-Nucleofector® System only requires 5 primary cell kits covering a broad range of primary cells. There are cell...
Missing: stable linesGuideline

Does your Nucleofector® Solutions contain anything that would inhibit attachment of adherent cells?

No. In many cases where decreased attachment is a problem, the cause is inactivated trypsin. Unless the trypsin is inactivated with trypsin inhibitor or media containg BSA or serum, it will continue to degrade the cells and ultimately decrease cell...
Missing: stable linesGuideline

Is the Human Leukocyte Antigen (HLA) type information available for any of Lonza's cell products?

Lonza does obtain the Human Leukocyte Antigen (HLA) type information for many (but not all) of the Clonetics® and Poietics® Cell Types we are offering. If you require cells of a specific HLA type donor, please contact Lonza's Scientific Support Team...
Missing: stable linesGuideline
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