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When performing the Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene recombinant Factor C (rFC), endotoxin detection kit how do I know my pipettes are working adequately?

Check your pipetting accuracy. This is important in every phase of LAL testing. Make sure you are adding the correct amounts of material. Good quality graduated pipettes are already calibrated by the manufacturer, but it is up to the user to use them...

When performing the Lonza Bacterial Endotoxin Test (BET), LAL gel clot endotoxin detection kit how do I know if I have a positive result for gel clot?

A positive result is a firm gel which maintains its integrity through inversion to 180°. Resist the temptation to look at any of the test tubes before the hour is up. When reading your test results after the one-hour incubation gently withdraw each...

How should I prepare and handle my product samples for Bacterial Endotoxin Testing (BET) when using Lonza endotoxin detection assays? How do I adjust the pH?

As with handling of the lysate test reagents, the key word is cleanliness. Use aseptic and apyrogenic technique. Check the product for inhibition. Some products, especially members of the small volume parenteral family, may contain substances at...

Are Lonza Bacterial Endotoxin Detection (BET) reagents FDA approved?

All Lonza LAL based products, as well as the facility they are produced in, are FDA licensed.

Are the horseshoe crabs killed or injured during the harvest process?

Lonza takes great care to not harm the crabs during the harvesting of their blood. During harvesting blood is collected by puncturing the septum on the underside of the animal with a small needle. The animal will only give as much blood as it can...

Does Lonza have products for oven validation depyrogenation studies?

Lonza offers Endotoxin Challenge Vials with part code 00192568 and High Potency Endotoxin with part code 00193763 for oven validation.

When preforming Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene recombinant Factor C (rFC), endotoxin detection kit to establish the potency of Endotoxin Challenge Vials (ECV) can I use a syringe to reconstitute the ECV and other assay reagents?

We do not recommend that a needle and syringe be used to reconstitute ECV or other LAL reagents. The plungers of plastic syringes are generally coated with a lubricant and tend to interfere with the assay. We recommend the tops be removed and diluent...

Where can I obtain a Certificate of Analysis (COA) for a product I purchased through Lonza?

So long as you have the product catalog number and batch/lot number, Lonza Certificates of Analysis (COA) can be downloaded from https://bioscience.lonza.com/lonza_bs/US/en/coa/search . Please enter the product catalog number and batch/lot number...

Which Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene Recombinant Factor C (rFC), endotoxin detection kit should I use?

There are a few different things you should consider before deciding which endotoxin detection kit to order. First of all, how many samples will you be testing, and how frequently? If you are going to be testing one or two samples, one time only,...

What equipment do I need to run my Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene Recombinant Factor C (rFC), endotoxin detection assay?

Each endotoxin detection assay requires the use of different equipment. If running one of our PYROGENT™ Gel-Clot assays, a non-circulating waterbath or a dry heat block able to heat at 37°C is required. If running one of our kinetic assays,...
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