Q:

When performing a Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor C (rFC), endotoxin detection assay what pH should my sample be? What should I adjust it with?

A:
Our endotoxin detection assays are optimized to work with samples with a pH range of 6-8. If the pH of your sample falls outside of this range you can adjust it with a buffer, or HCl or NaOH. Make sure the buffer you are using is endotoxin free by running it as a sample in your assay. A good choice is Lonza's 50 mM Tris Buffer which is tested to contain less than 0.005 EU/ml.
Related URLs:
Categories:
Endotoxin Detection
Research Areas:
Endotoxin Testing