Nucleofector I/II/2b

We have excellent data for the Nucleofection™ of Plasmodium berghei from the group Chris J. Janse and Andrew P. Waters from the University of Leiden. Their protocol describes a transfection efficiency of 10-3 – 10-4. With this protocol you can get more than 100 fold higher transfection efficiency compared to conventional electroporation, shorten the time period of selection and reduce the number of animals used for this research.

The complete detailed protocol is published online at Nature Protocols. The best combination for successful Nucleofection™ is the program U-033
(U-33 on Nucleofector™ I Device) in combination with the Basic Parasite Nucleofector™ Kit 2 (Lonza, cat. no. VMI-1021).This full text online version also contains 2 movies about the Nucleofection™ of parasites: High-efficiency transfection and drug selection of genetically transformed blood stages of the rodent malaria parasite Plasmodium berghei Authors: Janse CJ, Ramesar J and Waters AP In: Nat Protocols (2006) 1(1): 346-356

4D-Nucleofector:

Please note that it is not possible to simply translate the Nucleofector™ II/2b Programs to 4D-Nucleofector™ Programs. 
The difference between the metal electrodes used in the original system and the conductive polymer used in the new system require unique solutions and electrical parameters which must be determined empirically. However, customers using the 96-well Shuttle™ System have achieved results comparable to the published protocol and for some strains with even better viability. They used program FF-167 in combination with P5 Primary Cell Solution. As the 96-well Shuttle™
System also uses the conductive polymer electrodes, these conditions are transferable to the 4D-Nucleofector™ X Unit