The freeze medium for pMAT cells contain DMSO as a cryoprotectant that is toxic to thawed cells. To maintain full functionality of the pMAT cells, fast thawing and immediate dilution with complete medium is mandatory.

Briefly, use a water bath with a controlled temperature of 37°C and closely watch the thawing process. When you observe a remaining small ice crystal, transfer the vial contents into a 50 mL tube and immediately add the specified volume of complete medium drop-wise while swirling gently. Prolonged thawing and too fast addition of the medium impairs cell viability.

The dilution is sufficient. Washing of the cells may lead to stress and eventueally, cell loss and is not recommended.

Please also refer to the “Instructions for Routine Use of the PyroCell® MAT Kit” for detailed thawing instructions.