Alternative supplements may impact the reactivity of the pMAT cells towards endotoxin and Non-endotoxin Pyrogens (NEPs). If an alternative supplement is used, a new qualification of the MAT test is required. The optimized MAT cell culture supplement provided with the PyroCell® MAT Kit has been quality tested in MAT assays to ensure lot-to-lot consistency. Test results are available in a lot-specific certificate of analysis (CoA).