We recommend to store the unopened bottle of cell culture medium (IMDM) as stipulated by the manufacturer. An opened bottle shall be stored cooled (2-8°C).

Before use with MAT assays, the cell culture medium should be equilibrated to room temperature. Sterility of the cell culture medium must be ensured. Contaminated medium may contain floating particles or show turbidity. Do not use medium that shows signs of contamination.
Repeatedly warming / cooling the entire bottle with cell culture medium increases the likelihood of precipitate and may destroy growth promoting proteins contained in the media. To prevent repeated cooling and warming of the cell culture medium, suitable aliquots may be prepared.