Q:

Do you have any tips for the culture of primary cells or cell line prior to Nucleofection?

A:

Yes. Please see our Techincal Reference Guide which contains many helpful tips.

You can find additional information in the Optimized Nucleofection Protocol for your cell type of interest.

Related Literature:
CD-DS002_CellCultureTips_2019-05_sec.pdf
Categories:
Primary Cells and Media
Transfection
Cell Lines and Primary Cancer Cells
Research Areas:
Basic Research

You may also want to know:

What selection markers can I use for the generation of stable cell lines?
In stable cell line generation, I have a very high transfection efficiency, but most of my cells die during selection. Is this to be expected?
Generation of a stable cell line: A positive control plasmid works, but I cannot obtain stable clones with my construct of interest. What could be the reason?
How much DNA should I use in my Nucleofection® Reaction to obtain stable clones?
How long does it take to obtain stable transfectants?
Is your pmaxGFP control vector supplied with the Nucleofector® Kits suitable for stable expression?
How many stable clones will I get from one transfection?
Why is the Nucleofector® Technology ideal for protein production from stable clones ?
Why is the Nucleofector®Technology ideal for primary cells and difficult-to-transfect cell lines?
Following Nucleofection®, I cannot obtain stable clones from single cells. What could be the reason?
See More

Privacy | Legal | About Lonza

© Lonza. All rights reserved.