citations

                    Improvement of Protein Expression Profile in Three-Dimensional Renal Proximal Tubular Epithelial Cell Spheroids Selected Based on OAT1 Gene Expression: A Potential In Vitro Tool for Evaluating Human Renal Proximal Tubular Toxicity and Drug Disposition
                

Authors:

Naoki Ishiguro , Etsushi Takahashi , Hiroshi Arakawa , Asami Saito , Fumihiko Kitagawa , Masayuki Kondo , Gaku Morinaga , Masahito Takatani , Ryo Takahashi , Takashi Kudo , Shin-Ichi Mae , Moeno Kadoguchi , Daichi Higuchi , Yuya Nakazono , Ikumi Tamai , Kenji Osafune , Yoichi Jimbo

In:

Source: Drug Metabolism and Disposition
Publication Date: (2023)
Issue: 51(9): 1177-1187

Research Area:

Basic Research

Toxicology

Drug Discovery

Cells used in publication:

Renal proximal tubule cells (RPTEC), human: Species: human; Tissue Origin: kidney

Culture Media:

Renal Epithelial Cell Growth Medium

Experiment

spherical aggregates of RPTECs (1000cells / well ) were seeded mRNA of OAT 1 was checked and coompared with 2D culture. Different anounts of cells and different culture times were tested to estimate transporter expression of OAT1, OAT3, OCT2 and MATE1 etc.

Abstract

The proximal tubule plays an important role in the kidney and is a major site of drug interaction and toxicity. Analysis of kidney toxicity via in vitro assays is challenging, because only a few assays that reflect functions of drug transporters in renal proximal tubular epithelial cells (RPTECs) are available. In this study, we aimed to develop a simple and reproducible method for culturing RPTECs by monitoring organic anion transporter 1 (OAT1) as a selection marker. Culturing RPTECs in spherical cellular aggregates increased OAT1 protein expression, which was low in the conventional two-dimensional (2D) culture, to a level similar to that in human renal cortices. By proteome analysis, it was revealed that the expression of representative two proximal tubule markers was maintained and 3D spheroid culture improved the protein expression of approximately 7% of the 139 transporter proteins detected, and the expression of 2.3% of the 4,800 proteins detected increased by approximately fivefold that in human renal cortices. Furthermore, the expression levels of approximately 4,800 proteins in three-dimensional (3D) RPTEC spheroids (for 12 days) were maintained for over 20 days. Cisplatin and adefovir exhibited transporter-dependent ATP decreases in 3D RPTEC spheroids. These results indicate that the 3D RPTEC spheroids developed by monitoring OAT1 gene expression are a simple and reproducible in vitro experimental system with improved gene and protein expressions compared with 2D RPTECs and were more similar to that in human kidney cortices. Therefore, it can potentially be used for evaluating human renal proximal tubular toxicity and drug disposition. SIGNIFICANCE STATEMENT: This study developed a simple and reproducible spheroidal culture method with acceptable throughput using commercially available RPTECs by monitoring OAT1 gene expression. RPTECs cultured using this new method showed improved mRNA/protein expression profiles to those in 2D RPTECs and were more similar to those of human kidney cortices. This study provides a potential in vitro proximal tubule system for pharmacokinetic and toxicological evaluations during drug development.

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