Renal proximal tubule cells (RPTEC), human

RPTEC cells recover essential non-waste blood products from the passing filtrate and returns them to the circulation, produce vitamins or vitamin precursors, sense infectious and inflammatory components and release cytokines

Cell Type:
Kidney Epith.
Tissue Origin:
kidney
Species:
human
Research Area:
Dermatology/Tissue Engineering
Cell Characteristics:
Suspension

Recommended Media

REGM BulletKit is formulated to maintain and expand most renal epithelial cells.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Epithelial, basic U-017 2e6 70% 90% Plasmid (general) 5 µg 100 µl I/II/2b

Citations (34)

Categories:
Primary Cells and Media 
Authors:
Yao M, Rogers NM, Csányi G, Rodriguez AI, Ross MA, St Croix C, Knupp H, Novelli EM, Thomson AW, Pagano PJ, Isenberg JS 
In:
J Am Soc Nephrol (2014) 25(6): 1171-1186 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Lin J1, Deng Z1, Tanikawa C1, Shuin T2, Miki T3, Matsuda K1, Nakamura Y1 
In:
Int J Oncol. (2014) 44(5): 1490-8 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Wu HJ1, Yiu WH1, Li RX1, Wong DW1, Leung JC1, Chan LY1, Zhang Y2, Lian Q3, Lin M1, Tse HF2, Lai KN1, Tang SC1 
In:
PLoS ONE (2014) 9(3): e90883 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Uetake R1, Sakurai T1, Kamiyoshi A1, Ichikawa-Shindo Y1, Kawate H1, Iesato Y1, Yoshizawa T1, Koyama T1, Yang L1, Toriyama Y1, Yamauchi A1, Igarashi K1, Tanaka M1, Kuwabara T2, Mori K3, Yanagita M4, Mukoyama M2, Shindo T1. 
In:
PLoS ONE (2014) 9(2): e87667 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yiu WH1, Wong DW1, Chan LY1, Leung JC1, Chan KW2, Lan HY3, Lai KN1, Tang SC1. 
In:
PLoS ONE (2014) 9(2): e88894 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sciancalepore AG1, Sallustio F2, Girardo S3, Gioia Passione L4, Camposeo A5, Mele E1, Di Lorenzo M3, Costantino V6, Schena FP7, Pisignano D4. 
In:
PLoS ONE (2014) 9(1): e87496 
Categories:
 
Authors:
Iida R1, Ueki M2, Fujihara J3, Takeshita H3, Kimura-Kataoka K3, Yasuda T4. 
In:
Other (2013) 2(6): 440-7 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Wang F1, Flint OP 
In:
Antimicrob Agents Chemother (2013) 57(12): 6205-12 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Alvarez ML1, Khosroheidari M, Eddy E, Kiefer J. 
In:
PLoS ONE (2013) 8(10): e77468 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Stray KM1, Bam RA, Birkus G, Hao J, Lepist EI, Yant SR, Ray AS, Cihlar T 
In:
Antimicrob Agents Chemother (2013) 57(10): 4982-9 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Miyake M1, Goodison S, Lawton A, Zhang G, Gomes-Giacoia E, Rosser CJ 
In:
J Hematol Oncol (2013) 3(6): 65 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sallustio F1, Serino G, Costantino V, Curci C, Cox SN, De Palma G, Schena FP. 
In:
PLoS ONE (2013) 8(7): e68296 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Lednicky JA1, Waltzek TB, McGeehan E, Loeb JC, Hamilton SB, Luetke MC 
In:
Virol J (2013) 10(2013): 10.1186 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Zell S, Schmitt R, Witting S, Kreipe HH, Hussein K, Becker JU 
In:
Other (2013) 3(1): 50-8 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Zhou H1, Hasni SA, Perez P, Tandon M, Jang SI, Zheng C, Kopp JB, Austin H 3rd, Balow JE, Alevizos I, Illei GG 
In:
J Am Soc Nephrol (2013) 24(7): 1073-87 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chen NK1, Chong TW, Loh HL, Lim KH, Gan VH, Wang M, Kon OL 
In:
Other (2013) 91(5): 587-98 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Broekema NM1, Imperiale MJ. 
In:
Proc Natl Acad Sci USA (2013) 110(20): 8200-5 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Amato F1, Seia M, Giordano S, Elce A, Zarrilli F, Castaldo G, Tomaiuolo R 
In:
PLoS ONE (2013) 8(3): 60448 
Categories:
Primary Cells and Media 
Authors:
Storm P1, Klausen TK, Trulsson M, Ho C S J, Dosnon M, Westergren T, Chao Y, Rydström A, Yang H, Pedersen SF, Svanborg C 
In:
PLoS ONE (2013) 8(3): e58578 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
da Silva CG1, Maccariello ER, Wilson SW, Putheti P, Daniel S, Damrauer SM, Peterson CR, Siracuse JJ, Kaczmarek E, Ferran C 
In:
J Cell Physiol (2012) 227(4): 1382-90 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Thiyagarajan D1, Fismen S, Seredkina N, Jacobsen S, Elung-Jensen T, Kamper AL, Fenton CG, Rekvig OP, Mortensen ES. 
In:
PLoS ONE (2012) 7(3): e34080 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Giron-Michel J1, Azzi S, Khawam K, Mortier E, Caignard A, Devocelle A, Ferrini S, Croce M, François H, Lecru L, Charpentier B, Chouaib S, Azzarone B, Eid P 
In:
PLoS ONE (2012) 7(2): e31624 
Categories:
Primary Cells and Media 
Authors:
Matsuura K1, Nakada C, Mashio M, Narimatsu T, Yoshimoto T, Tanigawa M, Tsukamoto Y, Hijiya N, Takeuchi I, Nomura T, Sato F, Mimata H, Seto M, Moriyama M 
In:
BMC Cancer (2011) 11:523: 10.1186 
Categories:
Primary Cells and Media 
Authors:
James SH1, Hartline CB, Harden EA, Driebe EM, Schupp JM, Engelthaler DM, Keim PS, Bowlin TL, Kern ER, Prichard MN 
In:
Antimicrob Agents Chemother (2011) 55(10): 4682-91 
Categories:
Primary Cells and Media 
Authors:
Tran M1, Tam D, Bardia A, Bhasin M, Rowe GC, Kher A, Zsengeller ZK, Akhavan-Sharif MR, Khankin EV, Saintgeniez M, David S, Burstein D, Karumanchi SA, Stillman IE, Arany Z, Parikh SM. 
In:
J Clin Invest (2011) 121(10): 4003-14 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Domhan S1, Ma L, Tai A, Anaya Z, Beheshti A, Zeier M, Hlatky L, Abdollahi A 
In:
PLoS ONE (2011) 6: e21283 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Topalis D1, Lebeau I, Krecmerová M, Andrei G, Snoeck R 
In:
Antimicrob Agents Chemother (2011) 55(5): 1961-7 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Fuller CA1, Pellino CA, Flagler MJ, Strasser JE, Weiss AA 
In:
Infect Immun (2011) 79(3): 1329-37 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kimura H1, Li X, Torii K, Okada T, Kamiyama K, Mikami D, Kasuno K, Takahashi N, Yoshida H 
In:
Other (2011) 15(1): 34-40 
Categories:
Primary Cells and Media 
Authors:
Rinaldo CH1, Gosert R, Bernhoff E, Finstad S, Hirsch HH 
In:
Antimicrob Agents Chemother (2010) 54(11): 4714-22 
Categories:
Primary Cells and Media 
Authors:
Waalkes S1, Atschekzei F, Kramer MW, Hennenlotter J, Vetter G, Becker JU, Stenzl A, Merseburger AS, Schrader AJ, Kuczyk MA, Serth J 
In:
BMC Cancer (2010) 10(503): 1471 
Categories:
Primary Cells and Media 
Authors:
Bernhoff E1, Tylden GD, Kjerpeseth LJ, Gutteberg TJ, Hirsch HH, Rinaldo CH 
In:
J Virol (2010) 84(4): 2150-6 
Categories:
Primary Cells and Media 
Authors:
Maeda S1, Kobayashi MA, Araki S, Babazono T, Freedman BI, Bostrom MA, Cooke JN, Toyoda M, Umezono T, Tarnow L, Hansen T, Gaede P, Jorsal A, Ng DP, Ikeda M, Yanagimoto T, Tsunoda T, Unoki H, Kawai K, Imanishi M, Suzuki D, Shin HD, Park KS, Kashiwagi A, Iwamoto Y, Kaku K, Kawamori R, Parving HH, Bowden DW, Pedersen O, Nakamura Y 
In:
PLoS Genet (2010) 6(2): e1000842 
+ Show All