Renal proximal tubule cells (RPTEC), human

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RPTEC cells recover essential non-waste blood products from the passing filtrate and returns them to the circulation, produce vitamins or vitamin precursors, sense infectious and inflammatory components and release cytokines

Cell Type:
Kidney Epith.
Tissue Origin:
kidney
Species:
human
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Research Area:
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent

Recommended Media

Renal Epithelial Cell Growth Medium

REGM BulletKit is formulated to maintain and expand most renal epithelial cells.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Epithelial, basic U-017 2e6 70% 90% Plasmid (general) 5 µg 100 µl I/II/2b

Citations (39)

Improvement of Protein Expression Profile in Three-Dimensional Renal Proximal Tubular Epithelial Cell Spheroids Selected Based on OAT1 Gene Expression: A Potential In Vitro Tool for Evaluating Human Renal Proximal Tubular Toxicity and Drug Disposition
Categories:
Primary Cells and Media, 3D Cell Culture , Bioassay  
Authors:
Naoki Ishiguro , Etsushi Takahashi , Hiroshi Arakawa , Asami Saito , Fumihiko Kitagawa , Masayuki Kondo , Gaku Morinaga , Masahito Takatani , Ryo Takahashi , Takashi Kudo , Shin-Ichi Mae , Moeno Kadoguchi , Daichi Higuchi , Yuya Nakazono , Ikumi Tamai , Kenji Osafune , Yoichi Jimbo  
In:
Drug Metabolism and Disposition (2023) 51(9): 1177-1187 
Crabtree effect in kidney proximal tubule cells via late-stage glycolytic intermediates
Categories:
Primary Cells and Media, Bioassay  
Authors:
Manjula Darshi , Jana Tumova , Afaf Saliba , Jiwan Kim , Judy Baek , Subramaniam Pennathur , Kumar Sharma  
In:
iScience (2023) 26(4) : 1-14 
Effect of Replicative Senescence on the Expression and Function of Transporters in Human Proximal Renal Tubular Epithelial Cells
Categories:
Primary Cells and Media 
Authors:
Akimasa Sanagawa, Yuji Hotta , Rara Sezaki , Natsumi Tomita , Tomoya Kataoka , Yoko Furukawa-Hibi , Kazunori Kimura 
In:
Biol Pharm Bull (2022) 45(11): 1636-1643 
Technology Transfer of the Microphysiological Systems: A Case Study of the Human Proximal Tubule Tissue Chip
Categories:
Primary Cells and Media, 3D Cell Culture , Bioassay  
Authors:
Courtney Sakolish , Elijah J Weber , Edward J Kelly , Jonathan Himmelfarb , Roula Mouneimne , Fabian A Grimm , John S House , Terry Wade , Arum Han , Weihsueh A Chiu , Ivan Rusyn  
In:
Scientific Reports (2018) 8(1): 14882-14896 
Engineered extracellular matrices with controlled mechanics modulate renal proximal tubular cell epithelialization
Categories:
Primary Cells and Media, 3D Cell Culture , Bioassay  
Authors:
Jeffrey A Beamish , Evan Chen , Andrew J Putnam  
In:
PLoS ONE (2017) 12 (7): 1-24 
Thrombospondin-1 activation of signal-regulatory protein-a stimulates reactive oxygen species production and promotes renal ischemia reperfusion injury.
Categories:
Primary Cells and Media 
Authors:
Yao M, Rogers NM, Csányi G, Rodriguez AI, Ross MA, St Croix C, Knupp H, Novelli EM, Thomson AW, Pagano PJ, Isenberg JS 
In:
J Am Soc Nephrol (2014) 25(6): 1171-1186 
Downregulation of the tumor suppressor HSPB7, involved in the p53 pathway, in renal cell carcinoma by hypermethylation.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Lin J1, Deng Z1, Tanikawa C1, Shuin T2, Miki T3, Matsuda K1, Nakamura Y1 
In:
Int J Oncol. (2014) 44(5): 1490-8 
Mesenchymal stem cells modulate albumin-induced renal tubular inflammation and fibrosis
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Wu HJ1, Yiu WH1, Li RX1, Wong DW1, Leung JC1, Chan LY1, Zhang Y2, Lian Q3, Lin M1, Tse HF2, Lai KN1, Tang SC1 
In:
PLoS ONE (2014) 9(3): e90883 
Adrenomedullin-RAMP2 system suppresses ER stress-induced tubule cell death and is involved in kidney protection.
Categories:
Primary Cells and Media 
Authors:
Uetake R1, Sakurai T1, Kamiyoshi A1, Ichikawa-Shindo Y1, Kawate H1, Iesato Y1, Yoshizawa T1, Koyama T1, Yang L1, Toriyama Y1, Yamauchi A1, Igarashi K1, Tanaka M1, Kuwabara T2, Mori K3, Yanagita M4, Mukoyama M2, Shindo T1. 
In:
PLoS ONE (2014) 9(2): e87667 
Tissue kallikrein mediates pro-inflammatory pathways and activation of protease-activated receptor-4 in proximal tubular epithelial cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yiu WH1, Wong DW1, Chan LY1, Leung JC1, Chan KW2, Lan HY3, Lai KN1, Tang SC1. 
In:
PLoS ONE (2014) 9(2): e88894 
A Bioartificial Renal Tubule Device Embedding Human Renal Stem/Progenitor Cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sciancalepore AG1, Sallustio F2, Girardo S3, Gioia Passione L4, Camposeo A5, Mele E1, Di Lorenzo M3, Costantino V6, Schena FP7, Pisignano D4. 
In:
PLoS ONE (2014) 9(1): e87496 
Three Nonsynonymous Single Nucleotide Polymorphisms in the RhitH Gene Cause Reduction of the Repression Activity That Leads to Upregulation of M-LPH, a Participant in Mitochondrial Function
Categories:
 
Authors:
Iida R1, Ueki M2, Fujihara J3, Takeshita H3, Kimura-Kataoka K3, Yasuda T4. 
In:
Other (2013) 2(6): 440-7 
BMS-986001, an HIV nucleoside reverse transcriptase inhibitor, does not degrade mitochondrial DNA in long-term primary cultures of cells isolated from human kidney, muscle, and adipose tissue
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Wang F1, Flint OP 
In:
Antimicrob Agents Chemother (2013) 57(12): 6205-12 
Role of microRNA 1207-5P and its host gene, the long non-coding RNA Pvt1, as mediators of extracellular matrix accumulation in the kidney: implications for diabetic nephropathy.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Alvarez ML1, Khosroheidari M, Eddy E, Kiefer J. 
In:
PLoS ONE (2013) 8(10): e77468 
Evaluation of the effect of cobicistat on the in vitro renal transport and cytotoxicity potential of tenofovir
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Stray KM1, Bam RA, Birkus G, Hao J, Lepist EI, Yant SR, Ray AS, Cihlar T 
In:
Antimicrob Agents Chemother (2013) 57(10): 4982-9 
Erythropoietin is a JAK2 and ERK1/2 effector that can promote renal tumor cell proliferation under hypoxic conditions.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Miyake M1, Goodison S, Lawton A, Zhang G, Gomes-Giacoia E, Rosser CJ 
In:
J Hematol Oncol (2013) 3(6): 65 
miR-1915 and miR-1225-5p regulate the expression of CD133, PAX2 and TLR2 in adult renal progenitor cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sallustio F1, Serino G, Costantino V, Curci C, Cox SN, De Palma G, Schena FP. 
In:
PLoS ONE (2013) 8(7): e68296 
Isolation and genetic characterization of human coronavirus NL63 in primary human renal proximal tubular epithelial cells obtained from a commercial supplier, and confirmation of its replication in two different types of human primary kidney cells.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Lednicky JA1, Waltzek TB, McGeehan E, Loeb JC, Hamilton SB, Luetke MC 
In:
Virol J (2013) 10(2013): 10.1186 
Hypoxia Induces Mesenchymal Gene Expression in Renal Tubular Epithelial Cells: An in vitro Model of Kidney Transplant Fibrosis.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Zell S, Schmitt R, Witting S, Kreipe HH, Hussein K, Becker JU 
In:
Other (2013) 3(1): 50-8 
miR-150 promotes renal fibrosis in lupus nephritis by downregulating SOCS1.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Zhou H1, Hasni SA, Perez P, Tandon M, Jang SI, Zheng C, Kopp JB, Austin H 3rd, Balow JE, Alevizos I, Illei GG 
In:
J Am Soc Nephrol (2013) 24(7): 1073-87 
Negative regulatory responses to metabolically triggered inflammation impair renal epithelial immunity in diabetes mellitus
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chen NK1, Chong TW, Loh HL, Lim KH, Gan VH, Wang M, Kon OL 
In:
Other (2013) 91(5): 587-98 
miRNA regulation of BK polyomavirus replication during early infection.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Broekema NM1, Imperiale MJ. 
In:
Proc Natl Acad Sci USA (2013) 110(20): 8200-5 
Gene mutation in microRNA target sites of CFTR gene: a novel pathogenetic mechanism in cystic fibrosis?
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Amato F1, Seia M, Giordano S, Elce A, Zarrilli F, Castaldo G, Tomaiuolo R 
In:
PLoS ONE (2013) 8(3): 60448 
A unifying mechanism for cancer cell death through ion channel activation by HAMLET.
Categories:
Primary Cells and Media 
Authors:
Storm P1, Klausen TK, Trulsson M, Ho C S J, Dosnon M, Westergren T, Chao Y, Rydström A, Yang H, Pedersen SF, Svanborg C 
In:
PLoS ONE (2013) 8(3): e58578 
Hepatocyte growth factor preferentially activates the anti-inflammatory arm of NF-?B signaling to induce A20 and protect renal proximal tubular epithelial cells from inflammation
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
da Silva CG1, Maccariello ER, Wilson SW, Putheti P, Daniel S, Damrauer SM, Peterson CR, Siracuse JJ, Kaczmarek E, Ferran C 
In:
J Cell Physiol (2012) 227(4): 1382-90 
Silencing of renal DNaseI in murine lupus nephritis imposes exposure of large chromatin fragments and activation of Toll like receptors and the Clec4e
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Thiyagarajan D1, Fismen S, Seredkina N, Jacobsen S, Elung-Jensen T, Kamper AL, Fenton CG, Rekvig OP, Mortensen ES. 
In:
PLoS ONE (2012) 7(3): e34080 
Interleukin-15 plays a central role in human kidney physiology and cancer through the ?c signaling pathway
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Giron-Michel J1, Azzi S, Khawam K, Mortier E, Caignard A, Devocelle A, Ferrini S, Croce M, François H, Lecru L, Charpentier B, Chouaib S, Azzarone B, Eid P 
In:
PLoS ONE (2012) 7(2): e31624 
Downregulation of SAV1 plays a role in pathogenesis of high-grade clear cell renal cell carcinoma
Categories:
Primary Cells and Media 
Authors:
Matsuura K1, Nakada C, Mashio M, Narimatsu T, Yoshimoto T, Tanigawa M, Tsukamoto Y, Hijiya N, Takeuchi I, Nomura T, Sato F, Mimata H, Seto M, Moriyama M 
In:
BMC Cancer (2011) 11:523: 10.1186 
Cyclopropavir inhibits the normal function of the human cytomegalovirus UL97 kinase
Categories:
Primary Cells and Media 
Authors:
James SH1, Hartline CB, Harden EA, Driebe EM, Schupp JM, Engelthaler DM, Keim PS, Bowlin TL, Kern ER, Prichard MN 
In:
Antimicrob Agents Chemother (2011) 55(10): 4682-91 
PGC-1a promotes recovery after acute kidney injury during systemic inflammation in mice.
Categories:
Primary Cells and Media 
Authors:
Tran M1, Tam D, Bardia A, Bhasin M, Rowe GC, Kher A, Zsengeller ZK, Akhavan-Sharif MR, Khankin EV, Saintgeniez M, David S, Burstein D, Karumanchi SA, Stillman IE, Arany Z, Parikh SM. 
In:
J Clin Invest (2011) 121(10): 4003-14 
Intercellular communication by exchange of cytoplasmic material via tunneling nano-tube like structures in primary human renal epithelial cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Domhan S1, Ma L, Tai A, Anaya Z, Beheshti A, Zeier M, Hlatky L, Abdollahi A 
In:
PLoS ONE (2011) 6: e21283 
Activities of different classes of acyclic nucleoside phosphonates against BK virus in primary human renal cells.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Topalis D1, Lebeau I, Krecmerová M, Andrei G, Snoeck R 
In:
Antimicrob Agents Chemother (2011) 55(5): 1961-7 
Shiga toxin subtypes display dramatic differences in potency
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Fuller CA1, Pellino CA, Flagler MJ, Strasser JE, Weiss AA 
In:
Infect Immun (2011) 79(3): 1329-37 
Glucocorticoid enhances hypoxia- and/or transforming growth factor-ß-induced plasminogen activator inhibitor-1 production in human proximal renal tubular cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kimura H1, Li X, Torii K, Okada T, Kamiyama K, Mikami D, Kasuno K, Takahashi N, Yoshida H 
In:
Other (2011) 15(1): 34-40 
1-O-hexadecyloxypropyl cidofovir (CMX001) effectively inhibits polyomavirus BK replication in primary human renal tubular epithelial cells
Categories:
Primary Cells and Media 
Authors:
Rinaldo CH1, Gosert R, Bernhoff E, Finstad S, Hirsch HH 
In:
Antimicrob Agents Chemother (2010) 54(11): 4714-22 
Fibronectin 1 mRNA expression correlates with advanced disease in renal cancer
Categories:
Primary Cells and Media 
Authors:
Waalkes S1, Atschekzei F, Kramer MW, Hennenlotter J, Vetter G, Becker JU, Stenzl A, Merseburger AS, Schrader AJ, Kuczyk MA, Serth J 
In:
BMC Cancer (2010) 10(503): 1471 
Leflunomide inhibition of BK virus replication in renal tubular epithelial cells
Categories:
Primary Cells and Media 
Authors:
Bernhoff E1, Tylden GD, Kjerpeseth LJ, Gutteberg TJ, Hirsch HH, Rinaldo CH 
In:
J Virol (2010) 84(4): 2150-6 
A single nucleotide polymorphism within the acetyl-coenzyme A carboxylase beta gene is associated with proteinuria in patients with type 2 diabetes
Categories:
Primary Cells and Media 
Authors:
Maeda S1, Kobayashi MA, Araki S, Babazono T, Freedman BI, Bostrom MA, Cooke JN, Toyoda M, Umezono T, Tarnow L, Hansen T, Gaede P, Jorsal A, Ng DP, Ikeda M, Yanagimoto T, Tsunoda T, Unoki H, Kawai K, Imanishi M, Suzuki D, Shin HD, Park KS, Kashiwagi A, Iwamoto Y, Kaku K, Kawamori R, Parving HH, Bowden DW, Pedersen O, Nakamura Y 
In:
PLoS Genet (2010) 6(2): e1000842 
A novel in vitro system, the integrated discrete multiple organ cell culture (IdMOC) system, for the evaluation of human drug toxicity: comparative cytotoxicity of tamoxifen towards normal human cells from five major organs and MCF-7 adenocarcinoma breast cancer cells.
Categories:
Primary Cells and Media 
Authors:
Li AP1, Bode C, Sakai Y 
In:
Chem Biol (2004) 150(1): 129-36 
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