all cells transfected in solution SF:
Granta 519: DN-113: 85% TF
Z-138: CM-138: 86% TF
Jeko-1: DJ-105: 82% TF
Transfection of GRANTA-519, Z138, and JEKO-1 cells was carried out using the Amaxa 4D-Nucleofector (Lonza, Cologne, Germany). Briefly, 2.5 million cells were resuspended in SF solution (Lonza, Cologne, Germany) and mixed with 500 nM pool of siRNA (Sigma-Aldrich, St. Louis, MO). In each reaction, a scrambled sequence (SCR) and a GFP-producing plasmid were used as controls. Sequences of the siRNA pools, and the transfection programs used can be found in Supplemental Tables S3 and S4, respectively. Transient knock-down and overexpression of SOX11 was performed as described in Gustavsson et al.