A mantle cell lymphoma (MCL) cell line established from peripheral blood mononuclear cells of a patient with a large cell variant of MCL showing leukaemic conversion. [Jeon et al. Br J Haematol. 1998 Sep;102(5):1323-6]
RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640 when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cell cultures, including fresh human lymphocytes in the 72-hour phytohemagglutinin (PHA) stimulation assay.There are a variety of formulations:12-702 - with L-glutamineBE12-702/U1 - with UltraGlutamineIBE15-702D - [powder] with L-glutamine12-167 - without L-glutamine12-115 - with L-glutamine and 25 mM HEPES bufferBE12-115/U1 - with UltraGlutamineI and 25 mM HEPES buffer04-525 - with L-glutamine and 165 nM MOPS (used for some mycological assays)09-774 - with L-glutamine, 25 mM HEPES buffer 100 units/ml penicillin, 50 ug/ml streptomycin12-918 - without L-glutamine or phenol redBE12-752 - with L-glutamine, without D-glucoseStorage = 2ºC to 8ºC (versions without L-glutamine and those with UltraGlutamine can be stored at 15ºC to 30ºC)
Kuci V, Nordström L, Conrotto P, Ek S.
Chiron D, Dousset C, Brosseau C, Touzeau C, Maïga S, Moreau P, Pellat-Deceunynck C, Le Gouill S, Amiot M.
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