CytoSMART was used to record single images for creation of time-lapse videos of two different migration assays: Wound healing. PANC-1 cells (2 × 105 cells/well) were seeded on each side of a culture insert for live cell analysis. Inserts were placed in wells of a pre-coated 24-well plate (collagen IV, human cellular fibronectin and human plasma fibronectin for 2 h at 37 °C) and incubated at 37 °C and 5% CO2 to allow cells grow to confluence. Afterwards, inserts were removed with sterile tweezers to create a cell-free area of approximately 500 µm and cells were treated with NT4 peptide at different concentrations in complete medium. Cells were allowed to migrate in an appropriate incubator. CytoSMART Lux 10x System (Lonza) was used to take a picture at time point zero and every 10 min for a total of 22 h. The time lapse stacks of images were also analyzed using ImageJ and the two plug-ins: Manual Tracking, and Chemotaxis and Migration Tool. Individual cells were randomly selected and tracked throughout the 10 h time period. Cancer cell migration in Matrigel. PANC-1 cells (2 × 105 cells/well) were seeded on each side of a culture insert for live cell analysis. Inserts were placed in 24-well plate and incubated at 37 °C and 5% CO2 to allow cells grow to confluence. Inserts were removed with sterile tweezers to create a cell-free area of approximately 500 µm. Wells were covered with Matrigel for 30 min at 37 °C and then NT4 peptide in complete medium was added to each well. Cells were allowed to migrate in an appropriate incubator. CytoSMART Lux 10x System (Lonza) was used to take a picture at time point zero and every 7.5 min for a total of 42 h.