The novel gene fad104, containing a fibronectin type III domain, has a significant role in adipogenesis

Tominaga K, Kondo C, Johmura Y, Nishizuka M, Imagawa M
Source: FEBS Lett
Publication Date: (2004)
Issue: 577(1-2): 49-54
Research Area:
Cancer Research/Cell Biology
Cells used in publication:
3T3-L1 pre-ad
Species: mouse
Tissue Origin: embryo
Nucleofectorâ„¢ I/II/2b
A novel gene named fad104 (factor for adipocyte differentiation-104), whose expression level quickly increased in the early stage of adipogenesis, was isolated and characterized. The deduced amino acid sequence of fad104 revealed the possible presence of a fibronectin type III domain and transmembrane domain. The expression of fad104 was detected in adipocyte differentiable 3T3-L1 cells but not observed in the non-adipogenic cell line NIH-3T3. Moreover, the ability of 3T3-L1 cells to differentiate declined with the knockdown of fad104 by RNA interference, strongly indicating that fad104 functions as a positive regulator of adipogenesis.